following injection of LPS (Pruett, Fan et al. 2005). Finally, ethanol administered at 6g/kg but not 3g/kg by oral gavage in mice significantly increased serum concentrations of positive acute phase proteins amyloid A and P that arise early in the inflammatory response and recruit immune cells to the inflammatory site, indicating that ethanol modulates acute phase response in a dose-dependent manner (Pruett and Pruett 2006). This phenomenon was not observed in a TLR4 mutant mouse, indicating that the acute phase response is mediated by TLR4 (Pruett and Pruett 2006).
