Chunk #33 — Results — Organoids derived from schizophrenia iPSCs—dispersion of proliferating cells in the cortex and blockade of cortical neuronal development
To quantitatively assess changes involving the Ki67+ NPCs, we analyzed images of the organoids of three controls and three patients. Computer-based cell identification and counting showed a significant increase in the density of Ki67+ cells/area in the analyzed ROIs (Fig. 2b). For each ROI, Minimum Spanning Tree graphs were generated (Fig. 2, c1, c2), which showed significant increases in the distances between cells, i.e., increased NPC dispersion, in the schizophrenia organoids (Fig. 2, c3). Two-Way ANOVA demonstrated a highly significant relation between disease and cell migration.
