Chunk #32 — Results — Organoids derived from schizophrenia iPSCs—dispersion of proliferating cells in the cortex and blockade of cortical neuronal development
The impaired development of the schizophrenia cortical neurons was accompanied by a visible dispersion of the proliferating NPCs (Fig. 2a, b). To quantitatively asses these changes, we analyzed images of the organoids form three controls and three patients (Fig. 3). Computer-based cell identification and automated counting showed a significant increase in the density of Ki67+ cells/area of the schizophrenia ROIs (Fig. 3b). For each ROI (example in Fig. 3, c1), we generated Minimum Spanning Tree graphs (Fig. 3, c2) (Supplementary Methods). This analysis revealed significant increases in the distances between cells, i.e., NPC dispersion, in the schizophrenia organoids. Two-Way ANOVA demonstrated a highly significant relation between disease and cell migration (Fig. 3, c3).
