Methods, antibodies, and experimental controls are detailed in the eAppendix in the Supplement. Briefly, protein extraction and Western blotting were carried out using standard techniques. We included not only protein extracts from adult and fetal human brain but also protein extracts from transfected and nontransfected HEK293 cells9 and competent bacteria expressing recombinant ZNF804A. The main anti-ZNF804A antibody used was a polyclonal antibody mapping to an internal epitope of human ZNF804A (D-14; Santa Cruz Biotechnology).
