To visualize this, we calculated Z scores measuring the spatio-temporal specificity of gene expression for each SCZ-associated gene, across all 32 time-points (Figure 4). Genes clustered into four groups (Supplementary Figure 9), with distinct spatio-temporal expression signatures. The largest cluster (Cluster A, Figure 4a; 29 genes) spanned early to late-mid pre-natal development (4–24 weeks post conception (pcw)), either across the whole brain (22 genes) or in regions 1–3 only (7 genes). Twelve genes were expressed in late pre-natal development (Figure 4d; 25–38 pcw); 10 genes were expressed in regions 1–3, post-natally and in the late pre-natal period (Figure 4c), and 15 genes were expressed throughout development (Figure 4b), either specifically in region four (nine genes) or throughout the brain (six genes).
