IPA analysis of upstream regulators provides further evidence for a strong pro-inflammatory response: NFκB and 11 cytokines genes were activated and Il1rn (an antagonist of the IL1 receptor) was decreased (Supplementary Table 4). This analysis also provided additional evidence for increased signaling by CREB and cAMP, and evidence for increased calcium signaling. Growth factors Vegfa, TGFβ1, Bmp4, Kitlg, and Grp were all activated. Stearate biosynthesis and multiple cholesterol pathways, particularly those downstream of Srebf1 and Srebf2, all had decreased expression (Supplementary Table 4).
