There were 85 probe sets at the 1-hr time point, 169 probe sets at the 6-hr time point, and 113 probe sets at the 24-hr that were different (p < 0.01) between the water and ethanol groups. These probe-sets included named genes and ESTs. However, with a FDR = 0.25 adjustment, there were no significant differences at any of the time points. In addition, there was no effect of time within the water or ethanol groups. Therefore, an overall ethanol effect was determined by combining the data for the 3 time points into a single water group and a single ethanol group.
