In flies, alcohol sedation and/or the effects of alcohol on postural control can be assessed by exposing flies to alcohol vapor (which progressively increases their internal alcohol as occurs in humans when drinking alcohol) and then monitoring the ability of flies to move or remain standing over time (e.g., Bhandari et al., 2009; Lasek et al., 2011a; Maples and Rothenfluh, 2011; Rothenfluh et al., 2006; Sandhu et al., 2015; Schumann et al., 2011; Wen et al., 2005). Additionally, recovery from alcohol sedation is also a useful behavioral end point in flies (e.g., Cowmeadow et al., 2005; Ogueta et al., 2010). The locomotor stimulating effects of alcohol in flies can be assessed by exposing flies to alcohol vapor in conjunction with computer-based data analysis of video recordings of individual fly movement (Wolf et al., 2002). Furthermore, flies will preferentially consume food containing alcohol, allowing measurement of both volume and frequency of alcohol consumption (Devineni and Heberlein, 2009; Ja et al., 2007; Pohl et al., 2012; Shohat-Ophir et al., 2012; Xu et al., 2012), and this preference for alcohol can be modified
