Herein we provide an overview of the most recent, open-access version of the DrugBank knowledgebase. DrugBank 6.0 incorporates numerous enhancements and improvements over the previous version, including significantly more FDA-approved drugs (a 72% increase), many more investigational drugs (a 38% increase), a massive increase in catalogued drug–drug interactions (nearly 300%) and the number of tracked drug–food interactions (a 200% increase). In addition to this notable expansion in database size (Table 1), we have added thousands of new, colorful, richly annotated pathways depicting drug mechanisms and drug metabolism. These have been complemented by thousands of newly (and more accurately) predicted spectral data for 1D 1H and 13C NMR, LC–MS, GC–MS and related chromatographic data. DrugBank's layout has also been modified to incorporate new data fields and new kinds of data searches. Additionally, the DrugBank curation team has worked to improve data quality, by developing and refining data entry SOPs, improving training and expanding the number of curators. These updates and enhancements will be described in more detail under the following four headings: (i) Frontend Layout, Backend and Curation Protocols; (ii) Data
