Differences in cytoskeletal-related genes, proteins, and phosphorylated proteins, and an enrichment in BP and MF related to the cytoskeleton was also particularly prominent across analyses between the lines. For instance, Tuba1c, a globular protein which polymerizes to form a necessary component of microtubules, displayed the greatest differential log2 FC in gene expression in the RNA-sequencing analysis. Similarly, Mapt, Map2, Map4, Map6, Map1a, and Map1b among others emerged from the analyses as other microtubule associated proteins that were differentially expressed genes, proteins, and/or phosphorylated proteins between lines. Microtubule-associated protein 1B (Map1b) contains numerous phosphorylation sites that impact microtubule stability and dynamics (Yang et al. 2012) and is involved in both presynaptic and postsynaptic regulation particularly at glutamatergic synapses (Palenzuela et al. 2017; Eriksson et al. 2010; Bodaleo et al. 2016). In vitro work also suggests Map1b may facilitate the transport of the Nav1.6 voltage-gated sodium channel to the neuronal membrane which could impact action potential firing rates (O’Brien et al. 2012). As the Map1b gene and protein was significantly upregulated and contained 16 differentially expressed phosphopeptides in the DS of HAP
