To identify proteins whose genetically regulated expression is associated with TUD, we performed PWAS analyses by integrating TUD GWAS summary statistics and precomputed pQTLs from discovery (Banner)98,99 and validation (ROSMAP)100,101 datasets using the FUSION pipeline (http://gusevlab.org/projects/fusion/).45 Next, TWAS was performed using gene and splicing expression profiles measured in the adult DLPFC and gene expression profiles from the frontal cortex. Human brain transcriptome data, used as expression reference panels, were obtained from the CMC100 and GTEx frontal cortex v7.45,95 All tests were Bonferroni corrected for multiple testing (α = 0.05/N genes tested).
