To understand if the changes we observed for BD DG-like neurons appeared only during a narrow time window of cell maturation, we patch clamped the neurons from 10 to 45 days postdifferentiation, keeping the number of cells similar in each recording period between the three groups (control n = 190 cells from four lines, LR n = 155 cells from three lines and NR n = 112 cells from three lines). The number of evoked action potentials followed the BD general phenotype and was significantly increased for LR (37.5 ± 3.9) and NR (39.2 ± 5.4) DG-like neurons vs 20.6 ± 1.7 action potentials in control neurons (P<0.0001 for both LR vs control and NR vs control; Figure 3a). The sodium currents were similar for LR and control neurons but were ~ 45% lower for NR neurons (P<0.0001; Figure 3b). The fast potassium currents were ~ 8% increased in LR compared with control neurons (0 = 0.014, two-way analysis of variance) and ~ 5% increased in LR neurons compared with NR neurons (P = 0.0085) (Figure 3c). The slow potassium
