We prioritize genes for laboratory testing on the basis of evidence for SNV function (including coding variants, eQTLs and Hi-C interactions), biological support for relevance to BP (from literature review) and transgenic phenotype. We perform genotyping and Quantitative Reverse-Transcription Polymerase Chain Reaction (q RT-PCR) for the selected sentinel variants of interest using human vascular smooth muscle cells and endothelial cells and test for expression levels (Supplementary Note; Supplementary Table 31). All three SNVs were tested using an additive model.
