There were 76 genes including several potassium channel related genes (e.g., KCNS1, KCNS2, KCNA2) that showed significant spatial correlations with PC1 (Table S12). For the GO enrichment analysis, GCEA revealed that transcriptional correlations of PC1 were significantly (FDR-p<0.05) enriched to 403 out of 3646 categories of biological processes (Table S13), 85 out of 575 categories of molecular functions (Table S14), and 70 out of 422 categories of cellular components (Table S15). The top 30 enriched categories are shown in Figures S4–S6 respectively. Of note, the GO categories related to the synaptic ion channels, especially the potassium channels, were shown at the top of the enriched categories across three types of ontologies. For the enrichment analysis of developmental stages of the cortex, GCEA indicated that the PC1-related associations were over-represented at the developmental stages of late childhood, adolescence and young adulthood (Table S16).
