As mentioned before, the functional connectivity has been exhibited to be a potential method for the assessment of the organizational mechanisms of human brain in the fMRI field. To date, fOT method has been widely applied for resting-state functional brain mapping and connectivity studies. Previous studies of resting-state fOT have indicated the feasibility to assess resting-state functional connectivity by using hemodynamic response of brain [2, 20–24]. Although the resting-state in fOT fields with group-level analysis reveals the functional connectivity with inherent characteristic of the different brain systems such as the cognitive, sensorimotor, visual, auditory and language systems. However, the gender-related effects of the results in the group-level analysis with fOT measurement are unclear. The gender differences of structural and functional properties in human brain have reported in lots of previous studies of fMRI [25]. Many clinical studies of fMRI, EEG and fOT indicated the existed gender difference of brain functional connectivity in resting-state [26–28]. Nevertheless, there were few studies of gender-related effects in resting-state functional connectivity via fOT monitoring. Thus, we hope to further investigate the gender-related effects of PFC
