Chunk #20 — Results — The relative molecular mass (Mr) of MOPR in G/G mice was lower than that in A/A mice, which is due to differences in N-glycosylation of MOPR
Immunoblotting of the MOPR was performed on membranes of the thalamus and striatum of female mice. In the thalami of A/A or G/G mice, anti-mu C labeled several bands, one of which was absent in the MOPR(-/-) mice, indicating that this diffuse protein band represents the MOPR. Notably, the Mr range of the MOPR in A/A mice was higher than that in G/G mice: 56–67 kDa (median, 62 kDa) in the A/A mice (Fig. 2, left panel, lane 1 vs lane 3) and 51-62 kDa (median, 55 kDa) in G/G mice (Fig. 2, left panel, lane 2 vs lane 3). Again using the MOPR(-/-) mouse tissue as the negative control, we found that in the striata of A/A mice, the MOPR band labeled by anti-mu C was more diffuse [27], with a Mr range of 58–82 kDa (median, 72 kDa) (Fig. 2, right panel, lane 1 vs lane 3). Similarly, the striatal MOPR band in G/G mice had a lower Mr range, 53-76 kDa (median, 66 kDa) (Fig. 2, right panel, lane 2 vs lane 3). The same experiments were
