Based on the strong observed association with rs1229984 and rs2066702 we examined whether other variants affecting ADH1B expression (eQTLs) were also associated with AD using GTEx V7 results (https://www.gtexportal.org/)20. Three variants, rs11939328 (EU p = 0.78, AA p = 0.98, Trans p = 0.78), rs10516440 (EU p = 3.97E-6, AA p = 1.97E-3, Trans p = 4.72E-8), and rs7664780 (EU p = 0.87, AA p = 0.083, Trans p = 0.405), were selected after LD-informed clumping and the exclusion of variants in LD (r2>0.1) with the GWS coding alleles rs1229984 and rs2066702. Of these, only rs10516440 (AD conditional analyses: EU p = 1.34E-3, AA p = 0.013, Trans p = 7.44E-5) was a significant multi-tissue eQTL in random effects analysis for ADH1B (SFE = 319.4, SHet = 27.6, p = 1.4E-76), ADH1A (SFE = 139.4, SHet = 6.6, p = 6.72E-33), and ADH1C (SFE = 167.3, SHet = 8.9, p = 1.9E-39). Rs10516440 is a LD proxy (r2 > 0.9) of rs6827898 (Table 2) in populations of European and African descent. These variants are both located in an intergenic region
