Our result demonstrated that functional connectivity during resting-state condition of gender effects in the PFC region could be successfully measure by fOT method with high temporal resolution. Time-series analysis indicates the gender effects of functional connectivity and cortical lateralization and spectrum analysis indicates the significant differences in resting-state in PFC between male and female groups. The inferior PFC is significantly different between male and female groups with both time-series and spectrum analysis that can provide useful information for the future resting-state functional connectivity study with fOT-based measurement. Although there are still some limitations and unresolved issues [50], fOT provide high potential to be the ideal choice for resting-state functional studies in the fields of developmental and clinical neuroscience that can apply to several gender-related neurodegenerative diseases diagnosis.
