Using the bioinformatics software RepeatMasker and IntaRNA, we analyzed the putative SBS between LINC00665 and YY2, and luciferase gene reporter assays were used to precisely identify the SBS to clarify the interaction between LINC00665 and the YY2 mRNA 3′ UTR (Figure 5A). An RNA-IP assay was used to further investigate the effect of the combination of LINC00665, YY2, and STAU1, showing that the relative enrichment of both LINC00665 and YY2 was significantly increased in the anti-STAU1 group compared to those in the anti-IgG group (Figure 5B). Moreover, compared with the LINC00665+-NC group, overexpression of LINC00665 could crucially reduce the half-life of YY2 (Figure 5E), whereas STAU1 or UPF1 inhibition in glioma cells could substantially extend the half-life of YY2 mRNA (Figure 5D; Figures S3D–S3F).
