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Chunk #13 — Methods — Phenotyping for sucrose preferences, sweet food liking and sucrose analgesia

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Sweet preferences and analgesia during childhood: effects of family history of alcoholism and depression.
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standardize skin temperature. At 1.5 minutes subjects placed in their mouths, without swallowing, 15–20 ml of either a sucrose solution or water, in randomized order. Thirty seconds later, they transferred their hand to the cold-water bath (10.00 ± 0.01°C), continuing to bathe the taste receptors on the tongue with the solution. At fixed intervals (every 10 seconds) throughout the testing, subjects were encouraged and asked to keep their hand in the water bath as long as they possibly could. Each trial lasted a maximum of 4 minutes or until the subject withdrew the hand from the cold-water bath, whichever came first.