GO annotation of the SH DV genes revealed a significant enrichment in genes associated with cell-to-cell signaling (FDR < 2 × 10−4; Supplementary Table S6) and neuron projection (FDR < 7 × 10−3). The signaling genes (N = 33) in addition to the common DV genes noted above included Chat, Chrna7, Grik2, Grin2b Htr1b, Htr2a, Oprd1, Sv2c, and 10 protocadherins. The SH DV genes were enriched (p < 2 × 10−21) in a single network module, magenta; 187 or the 231 members of the magenta module were significantly DV between the High and Low selected lines. The magenta module was enriched in genes associated with cell-to-cell signaling (p < 3 × 10−11) and neuron part (p < 4 × 10−5; Supplementary Table S6). The magenta module signaling genes overlapped with those noted above, e.g., Chrna7 and Grin2b, and included 12 protocadherins. Focusing on the DV genes within the magenta module, average intramodular connectivity increased from 0.34 to 0.73 (Low vs. High; p < 1 × 10−63). Genes showing large changes (non-hub to hub status; Low vs. High) included Pde4d, Adra1a, Pcdhga8&6, and Ncam2.
