There were 1,392, 1,445, and 879 genes showing significant (adjusted p-value < 0.05) DW between the High and Low selected lines in the CeA, SH, and PL, respectively (Supplementary Table S7). The overlap in DW is illustrated in Figure 3D. Seventy-two genes were common to all three regions and this grouping was significantly (FDR < 5 × 10−3) enriched in genes associated with the post-synaptic component. Genes with this GO annotation included Chrna7, Als2, Pppir9a, Strn, Kcna4, Kif1a, and Slc1a2 (Supplementary Table S8). The overlap in genes between the PL and SH (N = 123) showed a significant (FDR < 2 × 10−2) enrichment in genes with the synaptic membrane annotation; these genes included Arrb1, Itgb1, Cpd, Akap5, Rim1, Shank3, Ptprz1, Gm3, Ank2, Gm1, and Cntmap2. There was no annotation enrichment in the overlapping genes between the PL and CeA. The overlapping genes between the CeA and the SH (N = 309) showed a significant (FDR < 2 × 10−3) enrichment in genes associated with the neuronal component (Supplementary Table S8); genes in this category (N = 60) included Calm1, Gad2, Nlg1, Oprd1, Pten, Rab10, and Sv2a.
