Assembly of functionally integrated human forebrain spheroids.

paper Cited Public

Authors: Birey, Fikri; Andersen, Jimena; Makinson, Christopher D; Islam, Saiful; Wei, Wu; Huber, Nina; Fan, H Christina; Metzler, Kimberly R Cordes; Panagiotakos, Georgia; Thom, Nicholas; O'Rourke, Nancy A; Steinmetz, Lars M; Bernstein, Jonathan A; Hallmayer, Joachim; Huguenard, John R; Paşca, Sergiu P
Year: 2017
Journal: Nature
PMID: 28445465
DOI: 10.1038/nature22330
PMCID: PMC5805137

Extended Data Figure 1

Immunostaining of hSS in cryosections showing PV neuronsTwo anti–PV antibodies have been used to validate specificity; co-localization with the neuronal marker DCX (day 209).

Extended Data Figure 10

Characterization of Dlxi1/2b::eGFP+ cells after migration(a) Representative images of 3D–reconstructed Dlxi1/2b::eGFP+ cell morphologies before and after migration from hSS into hCS. (b) Quantification of dendritic branching of Dlxi1/2b::eGFP+ cells in hSS (n= 58 cells) and in hCS (n= 55 cells) of fused hSS-hCS (two-way ANOVA; interaction F(2, 129)= 11.29, P< 0.001; Bonferroni post-hoc *P< 0.05, ***P< 0.001). (c) Representative examples of action potentials (slice recordings) in Dlxi1/2b::eGFP+ cells in unfused hSS, in hSS of fused hSS-hCS and in hCS after migration in fused hSS-hCS. (d) AT showing expression of the GABAergic synapse marker GPHN (green) colocalized with SYN1 (red) in hCS of fused hSS-hCS but not in unfused hCS; the glutamatergic marker PSD95 (cyan) colocalized with SYN1 is found in both fused and unfused hCS (equal volumes 1.2 μm deep). (e) AT of a Dlxi1/2b::eGFP+ synapse illustrating the colocalization with SYN1 (red), GPHN (cyan), and VGAT (white); 5 consecutive 70 nm sections (3 × 3 μm). (f) Representative examples of whole-cell voltage clamp recordings of IPSCs and EPSCs from Dlxi1/2b::eGFP+ cells in unfused hSS, in fused hCS-hSS, or after migration in hCS (g) Representative examples of whole-cell voltage clamp recordings of IPSCs and EPSCs in cells recorded from unfused hCS cells and fused hCS cells. (h) Electrical stimulation and patch clamp recording in fused hSS-hCS showing evoked EPSCs and IPSCs before (black) and after exposure to 10 μM gabazine (red). (i) Average peri-stimulus synaptic events (IPSCs and EPSCs) in Dlxi1/2::eGFP+ cells recorded in the hCS side of fused hSS-hCS before and after electrical stimulation (paired t-test, *P< 0.05). Data are mean ± s.e.m.

Figure 1

Characterization of hSS derived from hPSC(a) Generation of hCS and hSS. (b) Fold changes (relative to gene expression in hPSC; normalized to GAPDH) of NKX2–1 (n= 6 hPSC lines; Mann-Whitney test, P= 0.002), FOXG1 (n= 5 hPSC lines; t-test, P= 0.35) and EMX1 (n= 4 hPSC lines; Mann-Whitney test, P= 0.02) in hCS and hSS at day 25. (c, d) Immunostaining of hSS for NKX2–1, (e, f) GABA, GAD67 and MAP2, and (g, h) SST, CR, CB, PV. (i, j) Single cell profiling of hCS and hSS. (k) AT volume in hSS for MAP2, GFAP, SYN1 and VGAT. (l) Patch clamping in sliced hSS and a representative trace of whole-cell current-clamp recording. (m, n) Spontaneous IPSCs before (black) and during (blue) application of gabazine in an hSS slice (paired t-test, **P= 0.004).

Figure 2

Cell migration in fused hSS-hCS(a) Assembly of hCS and hSS. (b) Morphology before and after assembly. (c) Time-lapse of migration from hSS into hCS. (d) Assembly of hCS (AAV-hSYN1::mCherry) and hSS (Lenti-Dlxi1/2b::eGFP). (e) iDISCO-cleared hSS-hCS. (f, g) Saltatory migration of Dlxi1/2b::eGFP+ cells in fused hSS-hCS and (h) nucleokinesis. (I, j) Saltatory migration of Dlxi1/2b::eGFP+ cells in human fetal forebrain and (k) nucleokinesis.

Figure 3

Modeling of interneuron migration in hSS-hCS derived from Timothy syndrome(a) TS mutation in Cav1.2. (b) Calcium imaging in dissociated hSS (Ctrl: n= 38 cells from 2 subjects; TS: n= 68 cells from 2 subjects). (c) Migration of Dlxi1/2b::eGFP+ cells in TS and control hSS-hCS. (d, e) Number of saltations (Ctrl: n= 48 cells from 3 hiPSC lines derived from 3 subjects; TS: n= 51 cells from 3 hiPSC lines derived from 3 subjects; TS-Ctrl hybrid: n= 24 cells from 5 hiPSC line combinations from 2 TS and 2 Ctrl subjects), and saltation length (Ctrl: n= 21 cells from 3 hiPSC lines derived from 3 subjects; TS: n= 29 cells from 3 hiPSC derived from 3 subjects; TS-Ctrl hybrid: n= 12 cells from 3 hiPSC line combinations from 2 TS and 3 Ctrl subjects); one-way ANOVA with Dunnett’s multiple comparison test (***, P< 0.001). (f) Migration of TS and control Dlxi1/2b::eGFP+ cells in fused hSS-hCS (two-way ANOVA, interaction F(24, 408)= 17.71, P< 0.0001). (g) Saltation length following exposure to nimodipine (paired t-test; Ctrl: n= 13 cells from 3 hiPSC lines derived from 3 subjects, ***P< 0.001; TS: n= 12 cells from 2 hiPSC lines derived from 2 subjects, ***P< 0.001).

Figure 4

Functional integration of interneurons in fused hSS-hCS(a) Isolation of Dlxi1/2b::eGFP+ cells for transcriptional analysis. (b) t-SNE visualization of single cell gene expression at day 121 (4 weeks after hSS-hCS assembly). (c) Distribution across clusters (χ2-test, χ2= 43.39, P< 0.0001). (d) Expression of ERBB4, NXPH1, IGF1, TCF4, FOS, RAD1. (e) Morphology of Dlxi1/2b::eGFP+ cells before and after migration into hCS (f) Action potential generation in Dlxi1/2b::eGFP+ cells (one-way ANOVA, F(2, 30)= 1.25; ***P< 0.001; Bonferroni post-hoc, **P< 0.01; ***P< 0.001). (g) GABAergic synapse (by AT) on the pallial side of hCS-hSS with SYN1, GPHN and VGAT. (h) EPSCs and IPSCs in Dlxi1/2b::eGFP+ cells after migration. (i) Synaptic responses in Dlxi1/2b::eGFP+ cells (two-way ANOVA, interaction F(2, 61)= 18.46, P< 0.0001; Bonferroni post-hoc for EPSCs, ***P< 0.0001, **P< 0.001). (j) Synaptic responses in excitatory cells (two-way ANOVA, cortical neurons in hCS before and after assembly F(1, 26)= 5.6, P< 0.05; Bonferroni post-hoc for IPSC, *P< 0.05).

Extended Data Figure 2

Single cell gene expression of hCS and hSS at day 105 of differentiation (n= 11,838 cells; BD Resolve system)(a) Distribution of expression of the neuronal marker STMN2, (b) the progenitor marker VIM and of (c) a set of genes associated with the M cell cycle phase (AURKB, TPX2, UBE2C, HMMR, TOP2A, CCNB1, NUSAP1, NUF2, CDC6, HIST1H4C, BIRC5, CKS2). (d) Boxplots for genes enriched in each t-SNE cluster shown in Fig. 1j. (e–l) Top 25 genes in each of the 8 clusters shown in Fig. 1j (proportion of molecules per cells). (m) Scatter plot showing the number of genes detected versus the number of useful reads.

Extended Data Figure 3

Characterization of hSS differentiation conditions(a) Schematic illustrating the differentiation conditions for deriving hCS or hSS: IS, ISA and and ISRA. (b) Representative traces of intracellular calcium measurements (Fluo-4) demonstrating spontaneous activity in hSS at ~day 50 of differentiation. (c) Average calcium spike frequency in 3 distinct hSS differentiation conditions: IS (n= 114 cells), ISA (n= 327 cells), ISRA (n= 136 cells); cells derived from 3 hiPSC lines; one-way ANOVA, F(3, 719)= 5.86, ***P< 0.001. (d) Gene expression (qPCR, normalized to GAPDH) showing down-regulation of OCT4 and the lack of mesoderm (BRACH) and endoderm (SOX17) markers following differentiation of hiPSC into hCS and hSS conditions. (e) Gene expression (qPCR, fold change versus hiPSC and normalized to GAPDH) showing upregulation of forebrain markers (SIX3, FOXG1) but not midbrain (LMX1B), hypothalamus (RAX1) or spinal cord (HOXB4) markers. (f) Expression of ventral forebrain genes in hSS and hCS (qPCR, normalized to GAPDH) at day 25. (g) Average percentage of the proportion of NKX2–1 by immunostaining in dissociated hCS or hSS at day 25. (h) Expression of ventral forebrain genes in hSS (qPCR, normalized to GAPDH) at day 60. (i) Expression of glutamatergic, GABAergic, dopaminergic and cholinergic neurotransmitter identify genes in hSS (qPCR, normalized to GAPDH) at day 60. (j) Average percentage of the proportion of MAP2/Hoechst and GAD67/MAP2 by immunostaining in dissociated hSS at day 60. (k, l) Expression of interneuron subtypes genes and markers associated with interneuron migration in hSS (qPCR, normalized to GAPDH) at day 60. Number of lines hiPSC used indicated on each column. Data are mean ± s.e.m.

Extended Data Figure 4

Electrophysiological recordings of hCS and hSS(a) Representative EPSC traces of recordings from hCS neurons (sliced preparation) before (black) and during (green) exposure to the glutamate receptor blocker kynurenic acid (1 mM) (Mann-Whitney U-test, **P= 0.007). (b) Overlap of averaged EPSCs (red) recorded in hCS neurons (n= 6 cells) and averaged IPSCs (black) recorded in hSS (n= 5 cells). Data are mean ± s.d.

Extended Data Figure 5

Migration of Dlxi1/2::eGFP+ cells in fused hSS-hCS(a, b) Representative immunostaining in cryosections of hSS showing co-expression of Dlxi1/2::eGFP and GAD67 and GABA. (c) Quantification by immunostaining of the proportion of Dlxi1/2::eGFP+ cells that co-express GAD67 in hSS derived using the ISA or ISRA condition (n= 3 hiPSC lines; t-test, P= 0.35). (d) Proportion of Dlxi1/2::eGFP and GAD67 positive cells in hSS derived using the ISA or ISRA condition that co-express SST (t-test, P= 0.48), CR (t-test, *P= 0.04) or CB (t-test, P= 0.43); n = 3 hiPSC lines. (e) Representative images of hSS-hCS at day 60 showing migration of Dlxi1/2b::eGFP+ cells (from fluorescently labeled hSS) in fused hSS-hCS but not in hSS-hSS over time. (f) The number of Dlxi1/2b::eGFP+ (hSS-derived) or hSYN1::mCherry cells (hCS-derived) that moved in hSS-hCS or hSS-hSS was quantified in snapshots of live, intact spheroids at different time points (from day 3 to 25). The values shown are absolute number of cells that migrated to the other side (approximately the same area and thickness was imaged in each session); one-way ANOVA for cells at 17 days after assembly; F(2, 32)= 8.24, P= 0.001. (g) Representative images of fused hSS-hCS at day 91 showing migration of Dlxi1/2b::eGFP+ cells (from fluorescently labeled hSS) into fused hSS-hCS. (h) Representative image of an hSS that was plated on a glass coverslip and cultured for ~7 days. (i) Percentage of Dlxi1/2::eGFP inside the coverslip-plated hSS, in the rim (0–200 μm) or beyond this region (> 200 μm). (j) Quantification of the number of saltations of Dlxi1/2b::eGFP+ cells (n= 32 cells) inside the one-week coverslip-plated hSS, in the rim and outside this region (one-way ANOVA, interaction F (2, 30)= 22.12, P< 0.001; Bonferroni post-hoc ***P< 0.0001). (k) Diagram showing the angle of movement of migrating Dlxi1/2b::eGFP+ cells at 8–9 days after assembly of hSS-hCS. The angle was calculated between the leading process of Dlxi1/2b::eGFP+ cells that have moved into hCS and the fusion interface (n= 92 cells from 5 hiPSC lines). (l) Histogram showing the distribution of the distance of migrated Dlxi1/2b::eGFP+ cells relative to the edge of hCS in hSS-hCS at 30–50 days after assembly. The distance was measured in fixed 18 μm cryosections (n= 73 cells from 2 hiPSC lines). (m, n, o) Representative examples of Dlxi1/2b::eGFP+ cells migrated in the hCS that moved within a VZ-like region. The VZ-like region contains GFAP-expressing cells, is surrounded by TBR1+ cells and the migrated cells show GABA expression. Supplementary Video 2 shows movement of Dlxi1/2b::eGFP+ cells that is reminiscent of the ventricular-directed migration described in rodents.

Extended Data Figure 6

Single cell gene expression of Dlxi1/2b::eGFP+ cells in hSS and hCS (Smart-seq2)(a) Scheme showing the isolation by dissociation and fluorescence-activated cell sorting (FACS) of Dlxi1/2b::eGFP+ cells from hSS or hCS for single cell transcriptional analysis. (b) Violin plots showing expression in Dlxi1/2b::eGFP+ cells of selected genes associated with cortical, striatal and olfactory interneurons in hSS (light green, n= 123 cells) or hCS (dark green; n= 106 cells) at 2 weeks after assembly of hSS-hCS. (c) Violin plots showing expression in Dlxi1/2b::eGFP+ cells (at 4 weeks after assembly of hSS-hCS) in clusters A, B, and C (likelihood ratio test; GAD1, CELF4: P> 0.05; PBX3: P< e−7 for A versus B & C; NNAT: P< e−16 for C versus A & B, P< e−16 for B versus A & C; MALAT1: P< e−9 for C versus A & B; SOX11: P< e−16 for B versus A & C, P< e−9 for A versus B & C; GRIP2: P< e−8 for B versus A & C). (d) Scatter plot showing the number of genes detected (≥ 10 reads cutoff) versus the number of reads (n= 410 cells from combined single cell RNA-seq experiments after 2 weeks or 4 weeks of assembly in hSS-hCS). (e) Graph illustrating biologically variable transcripts (red circles) and non-variable transcripts (black circles) along with the technical noise from the ERCC spike in RNAs (blue dots). Green line shows the technical noise fit.

Extended Data Figure 7

Immunocytochemistry and pharmacology in fused hSS-hCS(a, b, c, d) Representative images of immunostainings for SST, GAD67, GABA, CR, CB in Dlxi1/2b::eGFP+ cells after migration in fused hSS-hCS. (e) Scheme illustrating the pharmacological manipulation of Dlxi1/2b::eGFP+ cells that are migrating in hSS-hCS. (f, g, h, i) Quantification of Dlxi1/2b::eGFP+ cell migration before and after exposure to 100 nM of the CXCR4 antagonist AMD3100 (n= 8 cells from 2 hiPSC lines; paired t-tests, *P= 0.03 for number of saltations, **P= 0.006 for saltation length, **P= 0.006 for speed when mobile, *P= 0.02 for path directness). (j) Plot illustrating the trajectory of Dlxi1/2b::eGFP+ cells in fused hSS-hCS before and after exposure to AMD3100. Data are mean ± s.e.m.

Extended Data Figure 8

Migration of Dlx2i1/2b::eGFP cells in mouse and human forebrain brain slices versus hSS-hCS(a, b, c) Representative images of human fetal cortex at GW20 showing immunostaining with antibodies against GFAP, BCL11B (CTIP2) and GABA. (d) Representative image showing cell labeling with the Dlx2i1/2b::eGFP reporter in human forebrain at GW18 (6 days after lentivirus infection) (e, f) Representative immunostainings in cryosections of human tissue at GW18 showing co-localization of Dlx2i1/2b::eGFP with NKX2-1 and GABA. (g) Representative images showing cell labeling with the Dlx2i1/2b::eGFP reporter in hSS-hCS (9 days after assembly), in human forebrain (GW18) and in mouse slice cultures (E18). (h, i) Comparison of Dlx2i1/2b::eGFP+ cell size and quantification of the ratio of soma diameter to the length of the leading process in fused hSS-hCS (n= 25 cells from 4 hiPSC lines), human forebrain at GW18 (n= 19 cells; black) and GW20 (n= 15 cells; gray), hSS-derived cells cultured on E14 mouse forebrain slices (n= 14 cells), and E18 mouse forebrain slices (n= 30 cells from 2 litters) (one-way ANOVA, interaction F(3, 97)= 11.61, P= 0.001, Bonferroni post-hoc ***P< 0.001, **P< 0.05). (j, k, l) Comparison of the number of saltations (n= 56 cells from 2 hiPSC lines; one-way ANOVA, interaction F(2, 103)= 29.27, P= 0.001, Bonferroni post-hoc ***P< 0.001), saltation length (n= 44 cells from 3 hiPSC lines; one-way ANOVA, interaction F(2, 91)= 3.0, P= 0.50), speed when mobile (n= 38 cells from 3 hiPSC lines; one-way ANOVA, interaction F(2, 83)= 11.38, P= 0.001, Bonferroni post-hoc ***P< 0.001) for Dlx2i1/2b::eGFP+ in fused hSS-hCS, human fetal forebrain (GW18: n= 19 cells; GW20: n= 15 cells), and E18 mouse forebrain slices (n= 14 cells for saltation length and speed, n= 16 cells for number of saltations from 2 litters). Data are mean ± s.e.m.

Extended Data Figure 9

Derivation of TS hSS, migration and electroporation(a) Sequencing of PCR-amplified DNA showing the p.G406R mutation in exon 8a of CACNA1C in TS (subject: 8303). (b) Representative images of hiPSC colonies expressing pluripotency markers (OCT4, SSEA4) in one TS subject (c) Level of gene expression (RT-qPCR, normalized to GAPDH) for NKX2-1 showing no defects in ventral forebrain induction in TS (two-way ANOVA; interaction F(2,15)= 0.20, P= 0.81; TS versus Ctrl F(1,15)= 0.16, P= 0.68). (d–g) Representative immunostainings in cryosections of TS hSS (day 60) showing expression of NKX2-1, GABA, MAP2, GAD67, SST and CR. (h) Calcium imaging (Fura-2) in dissociated hCS derived from TS subjects and controls (Ctrl: n= 81 cells from 2 subjects; TS: n= 147 cells from 2 subjects). Quantification of residual intracellular calcium ([Ca2+]i) following 67 mM KCl depolarization of Ctrl and TS cells in hCS cells. Residual [Ca2+]i was calculated by dividing the plateau calcium (C–A) level by the peak calcium elevation (B−A); (t-test, ***P< 0.001). (i) Quantification of [Ca2+]i following depolarization of Ctrl and TS cells in hSS (t-test, ***P< 0.001). (j) Representative image of fused TS hSS-hCS showing Dlxi1/2b::eGFP expression and migration. (k, l) Quantification of the number of saltations and saltation length of Dlx2i1/2b::eGFP cells in fused hSS-hCS across multiple Ctrl and TS lines (related to Fig. 3d, e). (m) Quantification of the speed when mobile of Dlxi1/2b::eGFP cells in fused hSS-hCS (Ctrl: n= 21 cells from 3 hiPSC lines derived from 3 subjects; TS: n= 29 cells from 3 hiPSC lines derived from 3 subjects; TS-Ctrl hybrid: n= 12 cells from 3 hiPSC line shown combinations; one-way ANOVA with Dunnett’s multiple comparison test; ***P< 0.001). (n) Electroporation of cDNA encoding the TS– and WT– CaV1.2 channels into slices of mouse E14 ganglionic eminences (GE). (o) Representative example of time-lapse live imaging depicting the saltatory migration of GFP+ cells in slices electroporated with CAG::GFP and either the WT–or the TS– CACNA1C. (p, q) Quantification of the number of saltations (t-test; **P< 0.01) and saltation length (t-test; ***P< 0.001) of GFP+ cells in electroporated mouse forebrain slices (WT: n= 33 cells; TS: n= 23 cells; from 3 litters). (r) Scheme illustrating pharmacological manipulation of LTCC during live imaging of fused hSS-hCS. (s) Quantification of speed when mobile following exposure to the LTCC blocker nimodipine (5 μM) (paired t-test; Ctrl: n= 13 cells from 3 hiPSC lines derived from 3 subjects, ***P< 0.001; TS: n= 12 cells from 2 hiPSC lines derived from 2 subjects, **P< 0.005). (t) Quantification of saltation length following exposure to roscovitine (15 μM) (paired t-test; Ctrl: n= 7 cells from 2 hiPSC lines derived from 2 subject, **P< 0.005; TS: n= 12 cells from 2 hiPSC lines derived from 2 subjects; ***P< 0.001). (u) Quantification of speed when mobile following exposure to roscovitine (15 μM) (paired t-tests; Ctrl: n= 9 cells from 2 hiPSC lines derived from 2 subjects, ***P< 0.001; TS: n= 12 cells from 2 hiPSC lines derived from 2 subjects; P= 0.05). Data are mean ± s.e.m.

#	Section	Preview
0	INTRODUCTION	The formation of the human cerebral cortex involves the assembly of circuits composed of…
1	INTRODUCTION	Here, we leverage a 3D differentiation approach using hPSCs to specify neural spheroids resembling…
2	GENERATION OF SUBDOMAIN-SPECIFIC FOREBRAIN SPHEROIDS	We have previously described the generation of floating, 3D neural cultures from hPSCs resembling…
3	GENERATION OF SUBDOMAIN-SPECIFIC FOREBRAIN SPHEROIDS	day 25 (Fig. 1c) but was distributed more broadly at later stages (Fig. 1d). At day 60, we observed…
4	GENERATION OF SUBDOMAIN-SPECIFIC FOREBRAIN SPHEROIDS	To comprehensively characterize hSS and hCS, we performed single cell transcriptional profiling at…
5	GENERATION OF SUBDOMAIN-SPECIFIC FOREBRAIN SPHEROIDS	In contrast, hSS included a cluster of ventral neural progenitors, a group of GABAergic cells…
6	GENERATION OF SUBDOMAIN-SPECIFIC FOREBRAIN SPHEROIDS	We next explored the functional properties of hSS. We found that 7 days of exposure to the…
7	ASSEMBLY OF FOREBRAIN SPHEROIDS	To develop a model for the migration of interneurons into the cerebral cortex, we placed hCS and hSS…
8	ASSEMBLY OF FOREBRAIN SPHEROIDS	into hCS (Fig. 2c; Supplementary Video 1). This movement was specific to fused hSS-hCS and…
9	ASSEMBLY OF FOREBRAIN SPHEROIDS	optical clearing (Fig. 2e). Interestingly, we also observed processes of Dlxi1/2b::eGFP+ cells that…
10	ASSEMBLY OF FOREBRAIN SPHEROIDS	We next used confocal imaging to capture the movement of Dlxi1/2b::eGFP-labelled cells in fused…
11	ASSEMBLY OF FOREBRAIN SPHEROIDS	We tested pharmacological manipulation of interneuron migration in fused hSS-hCS (Extended Data Fig.…
12	USING ASSEMBLED SPHEROIDS TO MODEL TIMOTHY SYNDROME	We next investigated whether assembled hSS-hCS could be used to model migration defects. Previous…
13	USING ASSEMBLED SPHEROIDS TO MODEL TIMOTHY SYNDROME	neurons compared to control cells (Fig. 3b; Extended Data Fig. 9h, i), similar to what we have…
14	USING ASSEMBLED SPHEROIDS TO MODEL TIMOTHY SYNDROME	observed a defect in mouse TS-CaV1.2 electroporated neurons displaying more frequent but shorter…
15	INTEGRATION OF INTERNEURONS IN ASSEMBLED SPHEROIDS	To investigate the hSS-derived neurons that migrated into hCS, we examined their single cell…
16	INTEGRATION OF INTERNEURONS IN ASSEMBLED SPHEROIDS	Dlxi1/2b::eGFP+ cells in hSS and in fused hSS-hCS. We found that hSS-derived cells that moved into…
17	INTEGRATION OF INTERNEURONS IN ASSEMBLED SPHEROIDS	Fig. 10e). To investigate the presence of functional synapses in migrated Dlxi1/2b::eGFP+ neurons,…
18	INTEGRATION OF INTERNEURONS IN ASSEMBLED SPHEROIDS	into hCS (Extended Data Fig. 10h, i). We found that electrical simulation evoked EPSCs (eEPSCs)…
19	DISCUSSION	We demonstrate the generation of a human 3D microphysiological system that includes…

Name	Type
3D cultures	drug
AAV-DJ1 local	drug
accutase	drug
aCSF	drug
adaptor local	drug
agarose	drug
Agilent 2100 Bioanalyzer	drug
AlexaFluo Dyes local	drug
Allopregnanolone local	drug
AMD3100 local	drug
anti–CTIP2 local	drug
anti–GFAP local	drug
anti–OCT4 local	drug
anti–SSEA4 local	drug
anti–TBR1 local	drug
Applied Biosystems	drug
Aquamount local	drug
artificial cerebrospinal fluid	drug
astroglia	anatomy
autism spectrum disorder	phenotype
autism spectrum disorders	phenotype
Axoscop II microscope local	drug
B-27 supplement	drug
Basal Medium Eagle local	drug
Bdnf	gene
BD Resolve single cell whole transcriptome amplification workflow local	drug
brain circuits	anatomy
brain tissue	anatomy
BSA	drug
CaCl2	drug
CACNA1C	gene
CAG-Cav1.2 plasmid local	drug
CAG::GFP plasmid local	drug
CALB1	gene
CALB2	gene
calcium	drug
calcium chloride	drug
Cav1.2-electroporated cells local	cohort
cDNA	drug
CELF4	gene
cell culture membrane inserts local	drug
cell identification local	phenotype
cesium chloride local	drug
Chat	gene
chicken anti–GFP antibody local	drug
choroid plexus	anatomy
Cl− reversal local	phenotype
control	cohort
control cells local	cohort
control hiPSC cohort local	cohort
control subjects	cohort
cortex	anatomy
cortical neurons	anatomy
Costar membrane inserts local	drug
CTIP2	gene
culture medium	drug
CXCR4	gene
DCX	gene
dendritic branching complexity local	phenotype
D-(+)-Glucose local	drug
dibenzyl ether local	drug
dichloromethane local	drug
dispase	drug
Dlg4	gene
Dlx1	gene
DLX1/2B local	gene
DLX1/2B::eGFP+ cells local	phenotype
Dlx2	gene
Dlx5	gene
DLX6	gene
Dlxi1/2b local	drug
Dlxi1/2b local	gene
Dlxi1/2b::eGFP local	drug
Dlxi1/2b::eGFP+ cells local	cohort
Dlxi1/2b::eGFP+ cells local	phenotype
Dlxi1/2b::eGFP lentivirus local	drug
Dlxi1/2b lentivirus local	drug
DMEM/F12	drug
dNTP mix local	drug
dorsal forebrain	anatomy
dorsal progenitors local	phenotype
dorsomorphin	drug
double maximum action potential generation rate local	phenotype
E13–14 mouse slices local	anatomy
E14–E18 mouse embryos local	cohort
E17–E18 slices local	anatomy
EBSS local	drug
EDTA	drug
eEPSCs local	phenotype
EGF	drug
eGFP	drug
EGTA	drug
embryonic forebrain local	anatomy
embryonic stem cells local	drug
Emx1	gene
epilepsy	phenotype
EPSCs	phenotype
ERBB4	gene
ERCC local	drug
ethanol consumption	phenotype
excitatory postsynaptic current (EPSC) local	phenotype
FBS	drug
FEZF2 local	gene
FGF2	drug
Fluo-4AM	drug
forebrain	anatomy
forebrain spheroids local	anatomy
Fos	gene
FOXG1	gene
frontal cortex	anatomy
full sample	cohort
Fura-2AM local	drug
fused hSS-hCS local	cohort
GABA	phenotype
GABAA receptor	drug
GABAergic cells local	anatomy
GABAergic interneuron subdomain local	anatomy
gabazine local	drug
Gabazine local	drug
GAD1	gene
GAD2	gene
ganglionic eminence local	anatomy
GeneTex GTX13970 local	drug
GFAP	gene
GFP	drug
GLAST1 local	gene
glucose	drug
glutamatergic neurons	phenotype
Glutamax	drug
glutamine	drug
glycine	drug
Gphn	gene
GRIP2 local	gene
GSX2 local	gene
GTP	drug
GW18 local	cohort
GW20 local	cohort
Hanks local	drug
HBSS	drug
hCS local	anatomy
hCS	cohort
hCS-derived cells local	cohort
heparin	drug
HEPES	drug
HEPES buffer	drug
HES6	gene
high-KCl Tyrode’s solution local	drug
High Output sequencing kits local	drug
High-Sensitivity DNA chip local	drug
High Sensitivity DNA Chip	drug
Hoechst 33258	drug
HOPX	gene
horse serum	drug
hPSC local	drug
hSS local	anatomy
hSS local	cohort
hSS-derived interneurons local	phenotype
hSS-hCS local	anatomy
hSS-IS local	cohort
hSS-ISA local	cohort
hSS-ISRA local	cohort
human brain	anatomy
human cells local	cohort
human cortical spheroids local	drug
human fetal Dlxi1/2b::eGFP–infected cells local	cohort
human forebrain tissue local	anatomy
human subpallium spheroids local	drug
hydrogen peroxide	drug
IGF1	gene
Illumina	drug
Illumina HiSeq 2500 instrument local	drug
ImageJ	drug
increased branching complexity local	phenotype
induced pluripotent stem cells	drug
inhibitory postsynaptic current (IPSC) local	phenotype
INSM1 local	gene
intermediate progenitors local	phenotype
interneuron	phenotype
interneuron migration	phenotype
interneuron motility local	phenotype
interneurons local	drug
intracellular calcium	phenotype
iPSCs	cohort
IS local	cohort
IWP-2 local	drug
KCl	drug
K-gluconate	drug
Knockout Serum local	drug
kynurenic acid local	drug
laminin	drug
Leica VT1200 vibratome local	drug
lentivirus	drug
Lenti-X concentrator local	drug
less efficient migration local	phenotype
LHX2	gene
Life Technologies	drug
Lipofectamine 2000	drug
low Cl− internal solution local	drug
LR White Resin local	drug
LTCC local	drug
L-type calcium channels local	drug
magnesium ATP local	drug
magnesium chloride	drug
MALAT1 local	gene
MAP2	gene
mCherry	drug
medial ganglionic eminence	anatomy
methanol	drug
MgCl2	drug
MgSO4	drug
mice	cohort
migration	phenotype
migration defect local	phenotype
migration speed local	phenotype
mitochondrial gene local	gene
mouse Dlxi1/2b::eGFP+ cells local	cohort
mouse ganglionic eminences local	anatomy
mouse interneurons local	phenotype
mouse slices local	anatomy
Mycoplasma local	drug
N-2 local	drug
NaH2PO4	drug
NaHCO3	drug
Nernst equation local	drug
Neurobasal-A local	drug
neurodevelopmental disorder	phenotype
neurons	phenotype
neuropsychiatric disorders	phenotype
Nextera XT library prep kit local	drug
NextSeq 500 local	drug
nimodipine	drug
NKX2-1	gene
NNAT	gene
non-essential amino acids	drug
normal goat serum	drug
NT3	drug
NXPH1	gene
OCT	drug
Oct4	gene
olfactory interneurons local	phenotype
Olig2	gene
oligodendrocyte progenitors local	anatomy
oligodendrocyte progenitors local	phenotype
Oligo-dT30VN local	drug
outer radial glia-like cells local	phenotype
pallium	anatomy
papain enzyme solution local	drug
paraformaldehyde	drug
path directness local	phenotype
patients	cohort
Pax6	gene
PBX3 local	gene
pCAGIG plasmid local	drug
PCR	drug
penicillin	drug
personalized human microphysiological systems local	drug
PFA	drug
p.G406R local	variant
pG406R local	variant
phosphate-buffered saline	drug
physiological recordings local	phenotype
poly-l-ornithine	drug
poly-ornithine	drug
poly-ornithine/laminin local	drug
primary human fetal brain tissue local	anatomy
PTwH local	drug
Pvalb	gene
Qiagen	drug
Qiagen elution buffer local	drug
QuantStudio RT-PCR software local	drug
Qubit dsDNA HS Assay kit local	drug
Qubit High Sensitivity DNA Assay local	drug
RASD1 local	gene
Reln	gene
retinoic acid	drug
RNase-Free DNase set	drug
RNase inhibitor	drug
RNeasy Mini kit	drug
Roche local	drug
roscovitine	drug
SAG	drug
saltation frequency local	phenotype
saltation length local	phenotype
saltation speed local	phenotype
SB-431542 local	drug
SCG2 local	gene
secondary antibody	drug
sequencing libraries local	drug
sIPSCs	phenotype
SLC13A4 local	gene
Slc17a6	gene
Slc17a7	gene
SLC32A1	gene
slicing local	phenotype
sodium phosphocreatine local	drug
Sox10	gene
SOX11	gene
SP8	gene
specific neuronal cell types local	anatomy
speed when mobile local	phenotype
spontaneous calcium activity local	phenotype
SSEA4 local	gene
Sst	gene
Stanford Gene Vector and Virus Core local	cohort
STMN2	gene
streptomycin	drug
striatal neurons local	phenotype
subdomains of the developing human brain local	anatomy
subpallium	anatomy
sucrose	drug
SuperScript III First-Strand Synthesis SuperMix local	drug
SYBR green	drug
SYN1	gene
Tbr1	gene
TBR2	gene
TCF7L2	gene
tetrahydrofuran local	drug
Th	gene
Timothy syndrome	phenotype
Tissue-Tek OCT Compound 4583 local	drug
Tourette syndrome	phenotype
Triton-X	drug
Triton X-100	drug
TruSeq Rapid kit local	drug
Trypsin inhibitor	drug
TS	cohort
TS-Cav1.2 local	variant
TS-CaV1.2 local	variant
TS gain-of-function mutation local	variant
TS hiPSC cohort local	cohort
TS patients local	cohort
TTR local	gene
Tween-20	drug
ventral forebrain local	anatomy
Ventral forebrain local	anatomy
ventral neural progenitors local	phenotype
ventricular zone	anatomy
VGAT	gene
vGLUT1	gene
ViiA7 machine local	drug
VZ-like region local	anatomy
WT-Cav1.2 local	variant
Y-27632 local	drug
β-mercaptoethanol	drug

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In this knowledge base

Title	Year	PMID
Genetics of Alcohol Use Disorder: A Role for Induced Pluripotent Stem Cells?	2018	29897633
Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration.	2017	28757360

External

Title	Authors	Journal	Year	Link
Aberrant calcium signaling and neuronal activity in the L271H CACNA1D (Cav1.3) iPSC model of neurodevelopmental disease.	Tisch M et al.	—	2026	→
Advances and applications of brain organoids in central nervous system disorders: Bridging the gap from laboratory to clinic.	Fang C et al.	—	2026	→
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Advances in the pathophysiological study of brain development: application of cerebral organoid combined with Spatial omics technology.	Wang J et al.	—	2026	→
Advances in three-dimensional modeling of ischemic injury.	Halkoluoto A et al.	—	2026	→
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Anesthetic-induced neurodevelopmental changes with region-specific responses to propofol in forebrain organoids.	She HQ et al.	—	2026	→
A novel iPSC model of Bryant-Li-Bhoj neurodevelopmental/neurodegenerative syndrome demonstrates the role of histone H3.3 in chromatin dynamics, neuronal differentiation, and maturation.	Sangree AK et al.	—	2026	→
Delayed forebrain excitatory and inhibitory neurogenesis in STRADA-related megalencephaly via mTOR hyperactivity.	Pan T et al.	—	2026	→
De novo H3.3K27M-altered diffuse midline glioma in human brainstem organoids to dissect GD2 CAR T cell function.	Bessler N et al.	—	2026	→
Dual developmental effects of ARX poly-alanine mutations on human cortical excitatory and inhibitory neurons.	Nieto-Estevez V et al.	—	2026	→
Generating Inhibitory Neuron Diversity through Morphogenic Patterning: From in vivo Studies to New in vitro Models.	Deutsch Guerrero T et al.	—	2026	→
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In vitro models of cell competition: current approaches and future directions.	Baglamis S et al.	—	2026	→
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Advancing ex vivo functional whole-organ prostate gland model for regeneration and drug screening.	Subbiahanadar Chelladurai K et al.	—	2025	→
An Efficient Organoid Cutting Method for Long-Term Culture and High-Throughput Analyses.	Chartrain NA et al.	—	2025	→
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Application of brain organoids in neurodevelopmental disorders.	Zhao C et al.	—	2025	→
Beyond consciousness: Ethical, legal, and social issues in human brain organoid research and application.	Kataoka M et al.	—	2025	→
Beyond Structure: Next-Generation Electrophysiological Platforms for Functional Brain Organoids.	Cha JH et al.	—	2025	→
Bioelectronic Interfaces and Sensors for Neural Organoids.	Wang Q et al.	—	2025	→
Bioengineering innovations for neural organoids with enhanced fidelity and function.	Sun Y et al.	—	2025	→
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Brain tissues, assemble! Inside the push to build better brain models.	Dolgin E	—	2025	→
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CRISPR-based genetically modified scaffold-free biomaterials for tissue engineering and regenerative medicine.	Chen Y et al.	—	2025	→
CRISPR screens in human neural organoids and assembloids.	Meng X et al.	—	2025	→
CXCR1 Depletion in Ly6C<sup>+</sup> cDC2 Alleviates Acute Lung Injury via Modulation of Th17/Treg Balance.	Li S et al.	—	2025	→
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Ex vivo functional whole organ in biomedical research: a review.	Subbiahanadar Chelladurai K et al.	—	2025	→
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Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1.	Yu D et al.	—	2025	→
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Modeling forebrain regional development and connectivity by human brain organoids.	Choe MS et al.	—	2025	→
Modelling human brain development and disease with organoids.	Birtele M et al.	—	2025	→
Modelling the human brain<i>in vitro</i>: biofabrication approaches for neural tissue engineering.	Borraccini A et al.	—	2025	→
Multi-organ model assessment of neurotoxicity following exposure of liver spheroids to drugs.	Wu W et al.	—	2025	→
Multiple sclerosis and infection: history, EBV, and the search for mechanism.	SoRelle ED et al.	—	2025	→
Multiplexing cortical brain organoids for the longitudinal dissection of developmental traits at single-cell resolution.	Caporale N et al.	—	2025	→
Muscle Organoid and Assembloid Systems.	Soydan HE et al.	—	2025	→
Neocortical neurogenesis: a proneural gene perspective.	Vasan L et al.	—	2025	→
Neurosteroid withdrawal disrupts GABAergic system development in human cortical organoids: implications for preterm birth.	Lacaille H et al.	—	2025	→
Next-Gen Stroke Models: The Promise of Assembloids and Organ-on-a-Chip Systems.	Lombardozzi G et al.	—	2025	→
Organoids in Genetic Disorders: from Disease Modeling to Translational Applications.	Zhu Y et al.	—	2025	→
Organoids - the future of pre-clinical development of AAV gene therapy for CNS disorders.	Kaiser VM et al.	—	2025	→
Peering into the mind: unraveling schizophrenia's secrets using models.	Nani JV et al.	—	2025	→
Personalized Human Astrocyte-Derived Region-Specific Forebrain Organoids Recapitulate Endogenous Pathological Features of Focal Cortical Dysplasia.	Xu J et al.	—	2025	→
Phenotypic complexities of rare heterozygous neurexin-1 deletions.	Fernando MB et al.	—	2025	→
Proof of concept for brain organoid-on-a-chip to create multiple domains in forebrain organoids.	Tsai YC et al.	—	2025	→
Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity.	Nityanandam A et al.	—	2025	→
Quantitative profiling of human brain organoid cell diversity across four protocols and multiple cell lines.	Naas J et al.	—	2025	→
Revolutionizing bladder cancer research: Harnessing 3D organoid technology to decode tumor heterogeneity and propel personalized therapeutics.	Kang H et al.	—	2025	→
SARS-CoV2 infection triggers inflammatory conditions and astrogliosis-related gene expression in long-term human cortical organoids.	Colinet M et al.	—	2025	→
Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids.	Saiki N et al.	—	2025	→
Single-cell technology for cell-based drug delivery and pharmaceutical research.	Hu H et al.	—	2025	→
Single rosette-based generation of uniform cortical assembloids recapitulating cellular interactions between neurons and glial cells.	Kim E et al.	—	2025	→
Skeletal muscle, neuromuscular organoids and assembloids: a scoping review.	Yang JL et al.	—	2025	→
Thalamus-cortex interactions drive cell type-specific cortical development in human pluripotent stem cell-derived assembloids.	Nishimura M et al.	—	2025	→
The emergence of electrical activity in human brain organoids.	Mancinelli S et al.	—	2025	→
The Evolving Landscape of Functional Models of Autism Spectrum Disorder.	Ranjan J et al.	—	2025	→
The promise of cerebral organoids for neonatology.	Howard CE et al.	—	2025	→
The Specification and Functional Maturation of Sub-Cerebral Projection Neurons Derived from Human Induced Pluripotent Stem Cells.	Jordan Z et al.	—	2025	→
Towards advanced regenerative therapeutics to tackle cardio-cerebrovascular diseases.	Chen X et al.	—	2025	→
Toxicity assessment using neural organoids: innovative approaches and challenges.	Park SH et al.	—	2025	→
Tumor-adipose assembloids reveal cell-fate-transition-triggered multistage collective invasions.	Lei M et al.	—	2025	→
Understanding monocyte-driven neuroinflammation in Alzheimer's disease using human cortical organoid microphysiological systems.	Tian C et al.	—	2025	→
Variability vs. phenotype: Multimodal analysis of Dravet syndrome brain organoids powered by deep learning.	Turpin-Moreno I et al.	—	2025	→
3D Multispheroid Assembly Strategies towards Tissue Engineering and Disease Modeling.	Zhu T et al.	—	2024	→
A 3D Bioprinted Cortical Organoid Platform for Modeling Human Brain Development.	Cadena MA et al.	—	2024	→
Advanced Cellular Models for Rare Disease Study: Exploring Neural, Muscle and Skeletal Organoids.	Bombieri C et al.	—	2024	→
Advances in human pluripotent stem cell reporter systems.	Puspita L et al.	—	2024	→
Advances in physiological and clinical relevance of hiPSC-derived brain models for precision medicine pipelines.	Imani Farahani N et al.	—	2024	→
A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis.	Fagiani F et al.	—	2024	→
A molecular and cellular perspective on human brain evolution and tempo.	Lindhout FW et al.	—	2024	→
A Multi-Electrode Array Platform for Modeling Epilepsy Using Human Pluripotent Stem Cell-Derived Brain Assembloids.	Pan T et al.	—	2024	→
An integrated transcriptomic cell atlas of human neural organoids.	He Z et al.	—	2024	→
Antisense oligonucleotide therapeutic approach for Timothy syndrome.	Chen X et al.	—	2024	→
A patient-specific lung cancer assembloid model with heterogeneous tumor microenvironments.	Zhang Y et al.	—	2024	→
A patterned human neural tube model using microfluidic gradients.	Xue X et al.	—	2024	→
A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids.	Bosone C et al.	—	2024	→
Applications of multiphoton microscopy in imaging cerebral and retinal organoids.	Lacin ME et al.	—	2024	→
Assembloid models of cell-cell interaction to study tissue and disease biology.	Onesto MM et al.	—	2024	→
Basic models to advanced systems: harnessing the power of organoids-based microphysiological models of the human brain.	Boylin K et al.	—	2024	→
Bioengineering toolkits for potentiating organoid therapeutics.	Park S et al.	—	2024	→
Bioprinting of Cells, Organoids and Organs-on-a-Chip Together with Hydrogels Improves Structural and Mechanical Cues.	Mierke CT	—	2024	→
Brain organoid methodologies to explore mechanisms of disease in progressive multiple sclerosis.	Simões-Abade MBC et al.	—	2024	→
Brain organoid protocols and limitations.	Zhao HH et al.	—	2024	→
Brain organoids: A new tool for modelling of neurodevelopmental disorders.	Aili Y et al.	—	2024	→
Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics.	Acharya P et al.	—	2024	→
Cell type specification and diversity in subpallial organoids.	Pavon N et al.	—	2024	→
Central nervous system vascularization in human embryos and neural organoids.	Boutom SM et al.	—	2024	→
Cerebral organoids display dynamic clonal growth and tunable tissue replenishment.	Lindenhofer D et al.	—	2024	→
Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids.	Kim HS et al.	—	2024	→
Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons.	Osaki T et al.	—	2024	→
Cortical brain organoid slices (cBOS) for the study of human neural cells in minimal networks.	Petersilie L et al.	—	2024	→
Cryopreservation of organoids: Strategies, innovation, and future prospects.	Han H et al.	—	2024	→
Deciphering potential vascularization factors of on-chip co-cultured hiPSC-derived cerebral organoids.	Shaji M et al.	—	2024	→
Decoding polygenic diseases: advances in noncoding variant prioritization and validation.	Chin IM et al.	—	2024	→
Design of neural organoids engineered by mechanical forces.	Suong DNA et al.	—	2024	→
Distinctive In Vitro Phenotypes in iPSC-Derived Neurons From Patients With Gain- and Loss-of-Function <i>SCN2A</i> Developmental and Epileptic Encephalopathy.	Mao M et al.	—	2024	→
Emergent trends in organ-on-a-chip applications for investigating metastasis within tumor microenvironment: A comprehensive bibliometric analysis.	He C et al.	—	2024	→
Engineering human midbrain organoid microphysiological systems to model prenatal PFOS exposure.	Tian C et al.	—	2024	→
Engineering immune organoids to regenerate host immune system.	Patel S et al.	—	2024	→
Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment.	Li M et al.	—	2024	→
Exploring the prospects, advancements, and challenges of <i>in vitro</i> modeling of the heart-brain axis.	Mabry SA et al.	—	2024	→
From wings to whiskers to stem cells: why every model matters in fragile X syndrome research.	Sandoval SO et al.	—	2024	→
Functional Neural Networks in Human Brain Organoids.	Gu L et al.	—	2024	→
Generating human neural diversity with a multiplexed morphogen screen in organoids.	Amin ND et al.	—	2024	→
Generation and characterization of cortical organoids from iPSC-derived dental pulp stem cells using traditional and innovative approaches.	Teles E Silva AL et al.	—	2024	→
Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.	Pang W et al.	—	2024	→
Generation of 'semi-guided' cortical organoids with complex neural oscillations.	Fitzgerald MQ et al.	—	2024	→
Genetics of human brain development.	Zhou Y et al.	—	2024	→
Gliomas: a reflection of temporal gliogenic principles.	Sojka C et al.	—	2024	→
Guidelines for Manufacturing and Application of Organoids: Brain.	Kwak T et al.	—	2024	→
Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.	Yang Z et al.	—	2024	→
Histone Trimethylations and HDAC5 Regulate Spheroid Subpopulation and Differentiation Signaling of Human Adipose-Derived Stem Cells.	Chang MM et al.	—	2024	→
Host circuit engagement of human cortical organoids transplanted in rodents.	Kelley KW et al.	—	2024	→
Human assembloids reveal the consequences of CACNA1G gene variants in the thalamocortical pathway.	Kim JI et al.	—	2024	→
Human Brain In Vitro Model for Pathogen Infection-Related Neurodegeneration Study.	Yan Y et al.	—	2024	→
Human brain organoids for understanding substance use disorders.	Li K et al.	—	2024	→
Human brain organoid: trends, evolution, and remaining challenges.	Li M et al.	—	2024	→
Human cerebral organoids: cellular composition and subcellular morphological features.	Mateos-Martínez P et al.	—	2024	→
Human fetal brain self-organizes into long-term expanding organoids.	Hendriks D et al.	—	2024	→
Humanized brain organoids-on-chip integrated with sensors for screening neuronal activity and neurotoxicity.	Saglam-Metiner P et al.	—	2024	→
<i>AAVS1</i>-targeted, stable expression of ChR2 in human brain organoids for consistent optogenetic control.	Hong S et al.	—	2024	→
Identity and Maturity of iPSC-Derived Oligodendrocytes in 2D and Organoid Systems.	Zeldich E et al.	—	2024	→
Investigating the neurobiology of maternal opioid use disorder and prenatal opioid exposure using brain organoid technology.	Dwivedi I et al.	—	2024	→
iPSC-derived models of PACS1 syndrome reveal transcriptional and functional deficits in neuron activity.	Rylaarsdam L et al.	—	2024	→
Isolated Fragments of Intact Microvessels: Tissue Vascularization, Modeling, and Therapeutics.	Strobel HA et al.	—	2024	→
Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids.	Yang X et al.	—	2024	→
Limitations of human brain organoids to study neurodegenerative diseases: a manual to survive.	Urrestizala-Arenaza N et al.	—	2024	→
Liver organoids: updates on generation strategies and biomedical applications.	Liu S et al.	—	2024	→
Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility.	Wang FC et al.	—	2024	→
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.	Chen Y et al.	—	2024	→
Making sense of Timothy syndrome with 3D human neuronal models.	Najm R et al.	—	2024	→
Meta-analysis of single-cell RNA sequencing co-expression in human neural organoids reveals their high variability in recapitulating primary tissue.	Werner JM et al.	—	2024	→
Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation.	Cassel de Camps C et al.	—	2024	→
Modeling early phenotypes of Parkinson's disease by age-induced midbrain-striatum assembloids.	Barmpa K et al.	—	2024	→
Modeling embryo-endometrial interface recapitulating human embryo implantation.	Shibata S et al.	—	2024	→
Modeling Parkinson's disease pathology in human dopaminergic neurons by sequential exposure to α-synuclein fibrils and proinflammatory cytokines.	Bayati A et al.	—	2024	→
Modeling tuberous sclerosis complex with human induced pluripotent stem cells.	Niu W et al.	—	2024	→
Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids as an evolving tool.	Rodrigues DB et al.	—	2024	→
Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters.	Park Y et al.	—	2024	→
Moral considerability of brain organoids from the perspective of computational architecture.	Boyd JL	—	2024	→
Multiscale engineering of brain organoids for disease modeling.	Xu C et al.	—	2024	→
Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights.	Lopez-Gordo E et al.	—	2024	→
Need of orthogonal approaches in neurological disease modeling in mouse.	Bossini L et al.	—	2024	→
Neurodevelopmental disorders: 2024 update.	Martínez de Lagrán M et al.	—	2024	→
Neuroimmune mechanisms in autism etiology - untangling a complex problem using human cellular models.	Vacharasin JM et al.	—	2024	→
Neuron-Astrocyte Interactions: A Human Perspective.	Pio T et al.	—	2024	→
Neuropathogenesis-on-chips for neurodegenerative diseases.	Amartumur S et al.	—	2024	→
Opportunities and Challenges in Advancing Plant Research with Single-cell Omics.	Rhaman MS et al.	—	2024	→
Organoid-based personalized medicine: from tumor outcome prediction to autologous transplantation.	Soto-Gamez A et al.	—	2024	→
Organoid intelligence for developmental neurotoxicity testing.	Alam El Din DM et al.	—	2024	→
Organoids and organ-on-chip technology for investigating host-microorganism interactions.	Walocha R et al.	—	2024	→
Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device.	Pavon N et al.	—	2024	→
Plant biotechnology research with single-cell transcriptome: recent advancements and prospects.	Ali M et al.	—	2024	→
Pluripotent stem cell-derived organoids: A brief history of curiosity-led discoveries.	Lancaster MA	—	2024	→
Polarization of organoids by bioengineered symmetry breaking.	Ryu JR et al.	—	2024	→
Probing the molecular and cellular pathological mechanisms of schizophrenia using human induced pluripotent stem cell models.	Sebastian R et al.	—	2024	→
Recapitulation and investigation of human brain development with neural organoids.	Tamada A et al.	—	2024	→
Recent advances and applications of human brain models.	Nishimura K et al.	—	2024	→
Research progress of brain organoids in the field of diabetes.	Su Y et al.	—	2024	→
Rigor and reproducibility in human brain organoid research: Where we are and where we need to go.	Sandoval SO et al.	—	2024	→
Self-Organization of Sinusoidal Vessels in Pluripotent Stem Cell-derived Human Liver Bud Organoids	Saiki N et al.	—	2024	—
Shaping the Neurovascular Unit Exploiting Human Brain Organoids.	Rizzuti M et al.	—	2024	→
Single-cell sequencing technology in skin wound healing.	Cheng XC et al.	—	2024	→
Single cell transcriptomics reveals distinct transcriptional responses to oxycodone and buprenorphine by iPSC-derived brain organoids from patients with opioid use disorder.	Ho MF et al.	—	2024	→
Spatially Self-Organized Three-Dimensional Neural Concentroid as a Novel Reductionist Humanized Model to Study Neurovascular Development.	Chai YC et al.	—	2024	→
Standardizing a method for functional assessment of neural networks in brain organoids.	Oliva MK et al.	—	2024	→
Strong Elastic Protein Nanosheets Enable the Culture and Differentiation of Induced Pluripotent Stem Cells on Microdroplets.	Mojares E et al.	—	2024	→
Sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids.	Boreland AJ et al.	—	2024	→
The Assembly of Miniaturized Droplets toward Functional Architectures.	Xu Y et al.	—	2024	→
The Promise and Potential of Brain Organoids.	Smirnova L et al.	—	2024	→
Trends and challenges in organoid modeling and expansion with pluripotent stem cells and somatic tissue.	Ge JY et al.	—	2024	→
Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate.	Acharya P et al.	—	2024	→
Unraveling radiation-induced skeletal muscle damage: Insights from a 3D human skeletal muscle organoid model.	Jiang Y et al.	—	2024	→
Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research.	Xiao W et al.	—	2024	→
Upregulated GIRK2 Counteracts Ethanol-Induced Changes in Excitability and Respiration in Human Neurons.	Prytkova I et al.	—	2024	→
16p11.2 deletion accelerates subpallial maturation and increases variability in human iPSC-derived ventral telencephalic organoids.	Fetit R et al.	—	2023	→
344Laser bioprinting of human iPSC-derived neural stem cells and neurons: Effect on cell survival, multipotency, differentiation, and neuronal activity.	Koch L et al.	—	2023	→
3D multicellular systems in disease modelling: From organoids to organ-on-chip.	Goldrick C et al.	—	2023	→
A beginner's guide on the use of brain organoids for neuroscientists: a systematic review.	Mulder LA et al.	—	2023	→
A Cross-Sectional Study of the Neuropsychiatric Phenotype of CACNA1C-Related Disorder.	Levy RJ et al.	—	2023	→
Advances and Applications of Brain Organoids.	Li Y et al.	—	2023	→
Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research.	Villanueva R	—	2023	→
Advancing organoid design through co-emergence, assembly, and bioengineering.	Tenreiro MF et al.	—	2023	→
Advancing preclinical models of psychiatric disorders with human brain organoid cultures.	Dixon TA et al.	—	2023	→
Affordable optical clearing and immunolabelling in mouse brain slices.	Muza PM et al.	—	2023	→
A genetics-first approach to understanding autism and schizophrenia spectrum disorders: the 22q11.2 deletion syndrome.	Fiksinski AM et al.	—	2023	→
A multi-disciplinary commentary on preclinical research to investigate vascular contributions to dementia.	Sri S et al.	—	2023	→
Animal Model Alternatives in Filovirus and Bornavirus Research.	Widerspick L et al.	—	2023	→
An update on stem cell and stem cell-derived extracellular vesicle-based therapy in the management of Alzheimer's disease.	Jeyaraman M et al.	—	2023	→
Application of colloidal photonic crystals in study of organoids.	Huang K et al.	—	2023	→
Application of Human Brain Organoids-Opportunities and Challenges in Modeling Human Brain Development and Neurodevelopmental Diseases.	Kim SH et al.	—	2023	→
A review of protocols for brain organoids and applications for disease modeling.	Mayhew CN et al.	—	2023	→
A review on current brain organoid technologies from a biomedical engineering perspective.	Lokai T et al.	—	2023	→
A review on in vitro model of the blood-brain barrier (BBB) based on hCMEC/D3 cells.	Qi D et al.	—	2023	→
A Robust Pipeline for the Multi-Stage Accelerated Differentiation of Functional 3D Cortical Organoids from Human Pluripotent Stem Cells.	Whye D et al.	—	2023	→
Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment.	Meng X et al.	—	2023	→
Balance between the cell viability and death in 3D.	Debruyne AC et al.	—	2023	→
Biological and structural phenotypes associated with neurodevelopmental outcomes in congenital heart disease.	Verrall CE et al.	—	2023	→
BOMA, a machine-learning framework for comparative gene expression analysis across brains and organoids.	He C et al.	—	2023	→
Brain assembloid: a human model for neural circuits research.	Wu S et al.	—	2023	→
Brain organoids and organoid intelligence from ethical, legal, and social points of view.	Hartung T et al.	—	2023	→
Brain organoids, consciousness, ethics and moral status.	Jeziorski J et al.	—	2023	→
Cell repelling agar@paper interface assisted probing of the tumor spheroids infiltrating natural killer cells.	Xie Y et al.	—	2023	→
Cell Replacement Therapy for Brain Repair: Recent Progress and Remaining Challenges for Treating Parkinson's Disease and Cortical Injury.	Harary PM et al.	—	2023	→
Cell reprogramming for oligodendrocytes: A review of protocols and their applications to disease modeling and cell-based remyelination therapies.	McCaughey-Chapman A et al.	—	2023	→
Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke.	Cao SY et al.	—	2023	→
Construction of a pancreatic cancer nerve invasion system using brain and pancreatic cancer organoids.	Song C et al.	—	2023	→
Developmental mechanisms underlying the evolution of human cortical circuits.	Vanderhaeghen P et al.	—	2023	→
Development and Application of Brain Region-Specific Organoids for Investigating Psychiatric Disorders.	Zhang Z et al.	—	2023	→
Dysregulation of CD177<sup>+</sup> neutrophils on intraepithelial lymphocytes exacerbates gut inflammation via decreasing microbiota-derived DMF.	Chen H et al.	—	2023	→
Electrophysiological and morphological characterization of single neurons in intact human brain organoids.	Landry CR et al.	—	2023	→
Engineering Biomimetic Microenvironment for Organoid.	Chen S et al.	—	2023	→
Engineering Human Brain Assembloids by Microfluidics.	Zhu Y et al.	—	2023	→
Establishing Hedgehog Gradients during Neural Development.	Douceau S et al.	—	2023	→
Evaluation of Neurotoxicity With Human Pluripotent Stem Cell-Derived Cerebral Organoids.	Parmentier T et al.	—	2023	→
Familial Alzheimer's disease-associated PSEN1 mutations affect neurodevelopment through increased Notch signaling.	Hurley EM et al.	—	2023	→
From 2D to 3D: Development of Monolayer Dopaminergic Neuronal and Midbrain Organoid Cultures for Parkinson's Disease Modeling and Regenerative Therapy.	Yeap YJ et al.	—	2023	→
From cells to insights: the power of human pluripotent stem cell-derived cortical interneurons in psychiatric disorder modeling.	Ni P et al.	—	2023	→
Functional reconstruction of the impaired cortex and motor function by hMGEOs transplantation in stroke.	Cao SY et al.	—	2023	→
Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.	Gabriel E et al.	—	2023	→
Generation of ventralized human thalamic organoids with thalamic reticular nucleus.	Kiral FR et al.	—	2023	→
Give Them Vasculature and Immune Cells: How to Fill the Gap of Organoids.	Yip S et al.	—	2023	→
Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly.	Zhang XS et al.	—	2023	→
Histone lysine methyltransferase-related neurodevelopmental disorders: current knowledge and saRNA future therapies.	Roth C et al.	—	2023	→
How might we build limbs <i>in vitro</i> informed by the modular aspects and tissue-dependency in limb development?	Tsutsumi R et al.	—	2023	→
Human 3D brain organoids: steering the demolecularization of brain and neurological diseases.	Adlakha YK	—	2023	→
Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer's Disease.	Young JE et al.	—	2023	→
Human pluripotent-stem-cell-derived organoids for drug discovery and evaluation.	Vandana JJ et al.	—	2023	→
Human pluripotent stem cell (hPSC) and organoid models of autism: opportunities and limitations.	Kilpatrick S et al.	—	2023	→
Hydrogel viscoelasticity modulates migration and fusion of mesenchymal stem cell spheroids.	Wu DT et al.	—	2023	→
Induced Pluripotent Stem Cell-Derived Organoids: Their Implication in COVID-19 Modeling.	Csöbönyeiová M et al.	—	2023	→
Inkjet-printed morphogenesis of tumor-stroma interface using bi-cellular bioinks of collagen-poly(N-isopropyl acrylamide-co-methyl methacrylate) mixture.	Cheng C et al.	—	2023	→
[Interdisciplinary approaches to brain organoid biology].	Kodera T et al.	—	2023	→
In vitro modeling of the human dopaminergic system using spatially arranged ventral midbrain-striatum-cortex assembloids.	Reumann D et al.	—	2023	→
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iPSCs-Derived Neurons and Brain Organoids from Patients.	Zhu W et al.	—	2023	→
LIF signaling regulates outer radial glial to interneuron fate during human cortical development.	Andrews MG et al.	—	2023	→
L-type calcium channels and neuropsychiatric diseases: Insights into genetic risk variant-associated genomic regulation and impact on brain development.	Baker MR et al.	—	2023	→
Mass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids.	Cappuccio G et al.	—	2023	→
Merits and challenges of iPSC-derived organoids for clinical applications.	Xu Z et al.	—	2023	→
Microfluidic Brain-on-a-Chip: From Key Technology to System Integration and Application.	Wang Z et al.	—	2023	→
Midbrain organoids-development and applications in Parkinson's disease.	Toh HSY et al.	—	2023	→
Modeling Autism Spectrum Disorders with Induced Pluripotent Stem Cell-Derived Brain Organoids.	Santos JLS et al.	—	2023	→
Modeling Human Brain Tumors and the Microenvironment Using Induced Pluripotent Stem Cells.	Khamis ZI et al.	—	2023	→
Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids.	Kodera T et al.	—	2023	→
Mutations in DARS2 result in global dysregulation of mRNA metabolism and splicing.	Guang S et al.	—	2023	→
[Nature and immune mechanisms of mental illnesses].	Priller J et al.	—	2023	→
Neural lineage differentiation of human pluripotent stem cells: Advances in disease modeling.	Yan YW et al.	—	2023	→
Non-cell-autonomous regulation of interneuron specification mediated by extracellular vesicles.	Pipicelli F et al.	—	2023	→
Novel model of cortical-meningeal organoid co-culture system improves human cortical brain organoid cytoarchitecture.	Jalilian E et al.	—	2023	→
Opportunities and limitations for studying neuropsychiatric disorders using patient-derived induced pluripotent stem cells.	Hong Y et al.	—	2023	→
Optically driven microtools with an antibody-immobilised surface for on-site cell assembly.	Mori S et al.	—	2023	→
Organ-on-Chip: Advancing Nutraceutical Testing for Improved Health Outcomes.	Farooqi MA et al.	—	2023	→
Patient Brain Organoids Identify a Link between the 16p11.2 Copy Number Variant and the <i>RBFOX1</i> Gene.	Kostic M et al.	—	2023	→
Present and Future Modeling of Human Psychiatric Connectopathies With Brain Organoids.	Urenda JP et al.	—	2023	→
Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches.	Newman PLH et al.	—	2023	→
Recent Advances in Brain Organoid Technology for Human Brain Research.	Jeong E et al.	—	2023	→
Recent advances in understanding neuronal diversity and neural circuit complexity across different brain regions using single-cell sequencing.	Xing Y et al.	—	2023	→
Shedding light on latent pathogenesis and pathophysiology of mental disorders: The potential of iPS cell technology.	Arioka Y et al.	—	2023	→
Simulating traumatic brain injury <i>in vitro</i>: developing high throughput models to test biomaterial based therapies.	Basit RH et al.	—	2023	→
Single-cell and spatial multi-omics in the plant sciences: Technical advances, applications, and perspectives.	Yu X et al.	—	2023	→
Single-Cell RNA-Seq Reveals Changes in Cell Subsets in the Cortical Microenvironment during Acute Phase of Ischemic Stroke Rats.	Zhao Y et al.	—	2023	→
Spatially controlled construction of assembloids using bioprinting.	Roth JG et al.	—	2023	→
Spatial transcriptomics in glioblastoma: is knowing the right zip code the key to the next therapeutic breakthrough?	Shireman JM et al.	—	2023	→
Stem Cell-Based Organoid Models of Neurodevelopmental Disorders.	Wang L et al.	—	2023	→
Structural and functional integration of human forebrain organoids with the injured adult rat visual system.	Jgamadze D et al.	—	2023	→
Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord-Like Organoid (pSCO) with Dorsoventral Organization.	Seo K et al.	—	2023	→
Temporal morphogen gradient-driven neural induction shapes single expanded neuroepithelium brain organoids with enhanced cortical identity.	Pagliaro A et al.	—	2023	→
The generation and properties of human cortical organoids as a disease model for malformations of cortical development.	Zhang XP et al.	—	2023	→
The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model.	Zayat V et al.	—	2023	→
Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models.	Urzì O et al.	—	2023	→
Toward the next generation of vascularized human neural organoids.	Li M et al.	—	2023	→
Unlocking Neural Function with 3D In Vitro Models: A Technical Review of Self-Assembled, Guided, and Bioprinted Brain Organoids and Their Applications in the Study of Neurodevelopmental and Neurodegenerative Disorders.	D'Antoni C et al.	—	2023	→
Vascularized human brain organoid on-chip.	Tan SY et al.	—	2023	→
Volumetric compression by heterogeneous scaffold embedding promotes cerebral organoid maturation and does not impede growth.	Tang X et al.	—	2023	→
What Have Organoids and Assembloids Taught Us About the Pathophysiology of Neuropsychiatric Disorders?	Levy RJ et al.	—	2023	→
3D and organoid culture in research: physiology, hereditary genetic diseases and cancer.	Suarez-Martinez E et al.	—	2022	→
A Comprehensive Update of Cerebral Organoids between Applications and Challenges.	Li X et al.	—	2022	→
Across Dimensions: Developing 2D and 3D Human iPSC-Based Models of Fragile X Syndrome.	Lee A et al.	—	2022	→
Advanced human developmental toxicity and teratogenicity assessment using human organoid models.	Li M et al.	—	2022	→
Advances in construction and modeling of functional neural circuits in vitro.	Chow SYA et al.	—	2022	→
Advances in Single-Cell Sequencing Technology and Its Application in Poultry Science.	Liu Y et al.	—	2022	→
Advances in stromal cell therapy for management of Alzheimer's disease.	Srivastava R et al.	—	2022	→
A matter of space and time: Emerging roles of disease-associated proteins in neural development.	Panagiotakos G et al.	—	2022	→
An in vitro model of neuronal ensembles.	Rabadan MA et al.	—	2022	→
Applications of Brain Organoids for Infectious Diseases.	Fan W et al.	—	2022	→
Approaches to investigating metabolism in human neurodevelopment using organoids: insights from intestinal and cancer studies.	Morales A et al.	—	2022	→
Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.	Verma SD et al.	—	2022	→
Bilirubin-Induced Neurological Damage: Current and Emerging iPSC-Derived Brain Organoid Models.	Pranty AI et al.	—	2022	→
Bioelectric Potential in Next-Generation Organoids: Electrical Stimulation to Enhance 3D Structures of the Central Nervous System.	O'Hara-Wright M et al.	—	2022	→
Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases.	Arthur P et al.	—	2022	→
Biomanufacturing human tissues via organ building blocks.	Wolf KJ et al.	—	2022	→
Brain-on-a-Chip: Dream or Reality?	Brofiga M et al.	—	2022	→
Buprenorphine Exposure Alters the Development and Migration of Interneurons in the Cortex.	Nieto-Estévez V et al.	—	2022	→
CACNA1C (Ca<sub>V</sub>1.2) and other L-type calcium channels in the pathophysiology and treatment of psychiatric disorders: Advances from functional genomics and pharmacoepidemiology.	Harrison PJ et al.	—	2022	→
Calcium and activity-dependent signaling in the developing cerebral cortex.	Arjun McKinney A et al.	—	2022	→
Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence.	Eke G et al.	—	2022	→
Central nervous system organoids for modeling neurodegenerative diseases.	Hou PS et al.	—	2022	→
Cerebral Organoids and Antisense Oligonucleotide Therapeutics: Challenges and Opportunities.	Lange J et al.	—	2022	→
Cerebral Organoids as an Experimental Platform for Human Neurogenomics.	Nowakowski TJ et al.	—	2022	→
Challenges and opportunities for precision medicine in neurodevelopmental disorders.	Chen GT et al.	—	2022	→
Challenges of Organoid Research.	Andrews MG et al.	—	2022	→
Challenges to, and prospects for, reverse engineering the gastrointestinal tract using organoids.	Kakni P et al.	—	2022	→
'Channeling' therapeutic discovery for epileptic encephalopathy through iPSC technologies.	Simkin D et al.	—	2022	→
Cortical Organoids to Model Microcephaly.	Farcy S et al.	—	2022	→
Differentiation of brain and retinal organoids from confluent cultures of pluripotent stem cells connected by nerve-like axonal projections of optic origin.	Fernando M et al.	—	2022	→
Dissecting the complexities of Alzheimer disease with in vitro models of the human brain.	Blanchard JW et al.	—	2022	→
Dissecting the molecular basis of human interneuron migration in forebrain assembloids from Timothy syndrome.	Birey F et al.	—	2022	→
Dysfunction of vesicular storage in young-onset Parkinson's patient-derived dopaminergic neurons and organoids revealed by single cell electrochemical cytometry.	Zhu W et al.	—	2022	→
Editorial: Improving <i>in vitro</i> modeling of human brain with future brain organoids.	Barral S et al.	—	2022	→
Electrophysiological Properties of Human Cortical Organoids: Current State of the Art and Future Directions.	Zourray C et al.	—	2022	→
Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells.	Allen GE et al.	—	2022	→
Engineering brain assembloids to interrogate human neural circuits.	Miura Y et al.	—	2022	→
Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture.	Jang H et al.	—	2022	→
Engineering multiscale structural orders for high-fidelity embryoids and organoids.	Shao Y et al.	—	2022	→
Fetal programming of human energy homeostasis brain networks: Issues and considerations.	Rasmussen JM et al.	—	2022	→
From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction.	Liu R et al.	—	2022	→
Functional imaging of brain organoids using high-density microelectrode arrays.	Schröter M et al.	—	2022	→
Functional neuronal circuitry and oscillatory dynamics in human brain organoids.	Sharf T et al.	—	2022	→
Generating Cerebral Organoids from Human Pluripotent Stem Cells.	Chew L et al.	—	2022	→
Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.	Anastasaki C et al.	—	2022	→
Genomics, convergent neuroscience and progress in understanding autism spectrum disorder.	Willsey HR et al.	—	2022	→
Gradient biomimetic platforms for neurogenesis studies.	Havins L et al.	—	2022	→
Harnessing the Power of Stem Cell Models to Study Shared Genetic Variants in Congenital Heart Diseases and Neurodevelopmental Disorders.	Chang X et al.	—	2022	→
Heart in a Dish: From Traditional 2D Differentiation Protocols to Cardiac Organoids.	Ramirez-Calderon G et al.	—	2022	→
Human assembloids.	Kanton S et al.	—	2022	→
Human brain organogenesis: Toward a cellular understanding of development and disease.	Kelley KW et al.	—	2022	→
Human Brain Organoid: A Versatile Tool for Modeling Neurodegeneration Diseases and for Drug Screening.	Ma C et al.	—	2022	→
Human cerebral organoids - a new tool for clinical neurology research.	Eichmüller OL et al.	—	2022	→
Human cerebral spheroids undergo 4-aminopyridine-induced, activity associated changes in cellular composition and microrna expression.	Parmentier T et al.	—	2022	→
Human forebrain organoids reveal connections between valproic acid exposure and autism risk.	Meng Q et al.	—	2022	→
Human mini brains and spinal cords in a dish: Modeling strategies, current challenges, and prospective advances.	Kofman S et al.	—	2022	→
Human neuromuscular junction three-dimensional organoid models and the insight in motor disorders.	Zhang K et al.	—	2022	→
Human organoids in basic research and clinical applications.	Tang XY et al.	—	2022	→
Human organoids: New strategies and methods for analyzing human development and disease.	Corsini NS et al.	—	2022	→
Imaging neuronal migration and network activity in human forebrain assembloids.	Birey F et al.	—	2022	→
Insights into SACS pathological attributes in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS)☆.	Aly KA et al.	—	2022	→
In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.	Saberi A et al.	—	2022	→
iPSC toolbox for understanding and repairing disrupted brain circuits in autism.	Chiola S et al.	—	2022	→
Label-free three-photon imaging of intact human cerebral organoids for tracking early events in brain development and deficits in Rett syndrome.	Yildirim M et al.	—	2022	→
Large-scale organoid study suggests effects of trisomy 21 on early fetal neurodevelopment are more subtle than variability between isogenic lines and experiments.	Czerminski JT et al.	—	2022	→
Large-Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors.	Ho DLL et al.	—	2022	→
Lineage recording in human cerebral organoids.	He Z et al.	—	2022	→
Maturation and circuit integration of transplanted human cortical organoids.	Revah O et al.	—	2022	→
Microfabricated disk technology: Rapid scale up in midbrain organoid generation.	Mohamed NV et al.	—	2022	→
Microfluidics for Neuronal Cell and Circuit Engineering.	Habibey R et al.	—	2022	→
Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality.	Sabate-Soler S et al.	—	2022	→
Mitigating Effect of Estrogen in Alzheimer's Disease-Mimicking Cerebral Organoid.	Kim JY et al.	—	2022	→
Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.	Li R et al.	—	2022	→
Modeling brain disorders using transplanted organoids: Beyond the short circuit.	Velasco S	—	2022	→
Modeling Developmental Brain Diseases Using Human Pluripotent Stem Cells-Derived Brain Organoids - Progress and Perspective.	Bhattacharya A et al.	—	2022	→
Modeling human neurodevelopmental diseases with brain organoids.	Lu X et al.	—	2022	→
Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes.	Wang Y et al.	—	2022	→
Modeling neurodegenerative diseases with cerebral organoids and other three-dimensional culture systems: focus on Alzheimer's disease.	Venkataraman L et al.	—	2022	→
Motor neuron-derived induced pluripotent stem cells as a drug screening platform for amyotrophic lateral sclerosis.	Amorós MA et al.	—	2022	→
Mouse embryo models built from stem cells take shape in a dish.	Amin ND et al.	—	2022	→
Multiscale Analysis of Cellular Composition and Morphology in Intact Cerebral Organoids.	Ma H et al.	—	2022	→
Neural Organoids, a Versatile Model for Neuroscience.	Lee JH et al.	—	2022	→
Neurodevelopmental copy-number variants: A roadmap to improving outcomes by uniting patient advocates, researchers, and clinicians for collective impact.	Commission on Novel Technologies for Neurodevelopmental Copy Number Variants	—	2022	→
Neuronal hyperexcitability and ion channel dysfunction in CDKL5-deficiency patient iPSC-derived cortical organoids.	Wu W et al.	—	2022	→
On-demand cell-autonomous gene therapy for brain circuit disorders.	Qiu Y et al.	—	2022	→
Organoid research on human early development and beyond.	Wen L et al.	—	2022	→
Organotypic and Microphysiological Human Tissue Models for Drug Discovery and Development-Current State-of-the-Art and Future Perspectives.	Youhanna S et al.	—	2022	→
Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder.	Lim ET et al.	—	2022	→
Orthogonally induced differentiation of stem cells for the programmatic patterning of vascularized organoids and bioprinted tissues.	Skylar-Scott MA et al.	—	2022	→
Patient-Derived Bladder Cancer Organoid Models in Tumor Biology and Drug Testing: A Systematic Review.	Medle B et al.	—	2022	→
Patterning of brain organoids derived from human pluripotent stem cells.	Zhang Z et al.	—	2022	→
Precision oncology using ex vivo technology: a step towards individualised cancer care?	Williams ST et al.	—	2022	→
Psychiatric risk gene transcription factor 4 preferentially regulates cortical interneuron neurogenesis during early brain development.	Wang Y et al.	—	2022	→
Pursuit of Perfection? On Brain Organoids as Models.	Gaillard M et al.	—	2022	→
Quickly moving too slowly: Interneuron migration in Timothy Syndrome.	Fernando MB et al.	—	2022	→
Reaching into the toolbox: Stem cell models to study neuropsychiatric disorders.	Whiteley JT et al.	—	2022	→
Redox modulation of stress resilience by Crocus sativus L. for potential neuroprotective and anti-neuroinflammatory applications in brain disorders: From molecular basis to therapy.	Scuto M et al.	—	2022	→
Regionally defined proteomic profiles of human cerebral tissue and organoids reveal conserved molecular modules of neurodevelopment.	Melliou S et al.	—	2022	→
Region Specific Brain Organoids to Study Neurodevelopmental Disorders.	Susaimanickam PJ et al.	—	2022	→
Research models of neurodevelopmental disorders: The right model in the right place.	Damianidou E et al.	—	2022	→
Revealing the Impact of Mitochondrial Fitness During Early Neural Development Using Human Brain Organoids.	Romero-Morales AI et al.	—	2022	→
Signalling pathways in autism spectrum disorder: mechanisms and therapeutic implications.	Jiang CC et al.	—	2022	→
Single-cell RNA sequencing reveals distinct transcriptional features of the purinergic signaling in mouse trigeminal ganglion.	Jia S et al.	—	2022	→
Stem Cell-Based Embryo Models: En Route to a Programmable Future.	Chen Y et al.	—	2022	→
Stem cell-based region-specific brain organoids: Novel models to understand neurodevelopmental defects.	Shafique S	—	2022	→
Stretchable mesh microelectronics for the biointegration and stimulation of human neural organoids.	Li TL et al.	—	2022	→
The Application of Brain Organoids in Assessing Neural Toxicity.	Fan P et al.	—	2022	→
The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.	Schembs L et al.	—	2022	→
The function of FUS in neurodevelopment revealed by the brain and spinal cord organoids.	Zou H et al.	—	2022	→
The importance of intermediate filaments in the shape maintenance of myoblast model tissues.	Nagle I et al.	—	2022	→
The promise of organoids for unraveling the proteomic landscape of the developing human brain.	Melliou S et al.	—	2022	→
Tissue Engineering Approaches to Uncover Therapeutic Targets for Endothelial Dysfunction in Pathological Microenvironments.	Ntekoumes D et al.	—	2022	→
Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.	Papes F et al.	—	2022	→
Translational organoid technology - the convergence of chemical, mechanical, and computational biology.	Zhang W et al.	—	2022	→
Translational pediatrics: clinical perspective for Phelan-McDermid syndrome and autism research.	Sakai Y et al.	—	2022	→
Understanding Primary Blast Injury: High Frequency Pressure Acutely Disrupts Neuronal Network Dynamics in Cerebral Organoids.	Silvosa MJ et al.	—	2022	→
Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies.	Choy Buentello D et al.	—	2022	→
Using Stem Cell Models to Explore the Genetics Underlying Psychiatric Disorders: Linking Risk Variants, Genes, and Biology in Brain Disease.	Brennand KJ	—	2022	→
What Makes Organoids Good Models of Human Neurogenesis?	Yang Q et al.	—	2022	→
WITHDRAWN: Micro- and nanodevices for integration with human brain organoids.	Tran HN et al.	—	2022	→
3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels.	Daly AC et al.	—	2021	→
3D-printed microplate inserts for long term high-resolution imaging of live brain organoids.	Oksdath Mansilla M et al.	—	2021	→
A comprehensive library of human transcription factors for cell fate engineering.	Ng AHM et al.	—	2021	→
Advances in Central Nervous System Organoids: A Focus on Organoid-Based Models for Motor Neuron Disease.	Vieira de Sá R et al.	—	2021	→
Advances in development and application of human organoids.	Shankaran A et al.	—	2021	→
Advances in modelling the human microbiome-gut-brain axis in vitro.	Moysidou CM et al.	—	2021	→
Advancing models of neural development with biomaterials.	Roth JG et al.	—	2021	→
A guidebook for DISCO tissue clearing.	Molbay M et al.	—	2021	→
A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies.	Kang Y et al.	—	2021	→
A look into retinal organoids: methods, analytical techniques, and applications.	Afanasyeva TAV et al.	—	2021	→
A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging.	Khan I et al.	—	2021	→
An Individual Patient's "Body" on Chips-How Organismoid Theory Can Translate Into Your Personal Precision Therapy Approach.	Marx U et al.	—	2021	→
Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids.	Adhya D et al.	—	2021	→
Assembly of Multi-Spheroid Cellular Architectures by Programmable Droplet Merging.	Cui H et al.	—	2021	→
Atypical Neurogenesis in Induced Pluripotent Stem Cells From Autistic Individuals.	Adhya D et al.	—	2021	→
BASP1 labels neural stem cells in the neurogenic niches of mammalian brain.	Manganas LN et al.	—	2021	→
Behavior and lineage progression of neural progenitors in the mammalian cortex.	Lin Y et al.	—	2021	→
Bioengineering tissue morphogenesis and function in human neural organoids.	Fedorchak NJ et al.	—	2021	→
Biomaterial-guided stem cell organoid engineering for modeling development and diseases.	Hoang P et al.	—	2021	→
Bioprinting for the Biologist.	Daly AC et al.	—	2021	→
Brain organoid: a 3D technology for investigating cellular composition and interactions in human neurological development and disease models in vitro.	Agboola OS et al.	—	2021	→
Brain organoids: an ensemble of bioassays to investigate human neurodevelopment and disease.	Sidhaye J et al.	—	2021	→
Brain organoids: A new frontier of human neuroscience research.	Lancaster MA	—	2021	→
Brain Organoids: Filling the Need for a Human Model of Neurological Disorder.	Jalink P et al.	—	2021	→
Brain Organoids: Studying Human Brain Development and Diseases in a Dish.	Xu J et al.	—	2021	→
Brain Organoids: Tiny Mirrors of Human Neurodevelopment and Neurological Disorders.	Yadav A et al.	—	2021	→
Building inner ears: recent advances and future challenges for in vitro organoid systems.	van der Valk WH et al.	—	2021	→
Building on a Solid Foundation: Adding Relevance and Reproducibility to Neurological Modeling Using Human Pluripotent Stem Cells.	Knock E et al.	—	2021	→
Building the brain from scratch: Engineering region-specific brain organoids from human stem cells to study neural development and disease.	Jacob F et al.	—	2021	→
Cell Therapy for Stroke: A Mechanistic Analysis.	Gu BJ et al.	—	2021	→
Cellular complexity in brain organoids: Current progress and unsolved issues.	Mansour AA et al.	—	2021	→
Cerebral Organoids-Challenges to Establish a Brain Prototype.	Eremeev AV et al.	—	2021	→
Cerebral organoids to unravel the mechanisms underlying malformations of human cortical development.	Krefft O et al.	—	2021	→
Characterization of the heterogeneity of endothelial cells in bleomycin-induced lung fibrosis using single-cell RNA sequencing.	Liu X et al.	—	2021	→
Classics never get old: neurotransmitters shape human cortical interneuron migration.	Bonafina A et al.	—	2021	→
Complex Organ Construction from Human Pluripotent Stem Cells for Biological Research and Disease Modeling with New Emerging Techniques.	Matsumoto R et al.	—	2021	→
'Consciousnessoids': clues and insights from human cerebral organoids for the study of consciousness.	Lavazza A	—	2021	→
Controllable fusion of human brain organoids using acoustofluidics.	Ao Z et al.	—	2021	→
Cortical Cartography: Mapping Arealization Using Single-Cell Omics Technology.	Nano PR et al.	—	2021	→
Cortical organoids model early brain development disrupted by 16p11.2 copy number variants in autism.	Urresti J et al.	—	2021	→
Deciphering and reconstitution of positional information in the human brain development.	Wang YF et al.	—	2021	→
Deconstructing and reconstructing the human brain with regionally specified brain organoids.	Xiang Y et al.	—	2021	→
Defense of COVID-19 by Human Organoids.	Lv T et al.	—	2021	→
Dopamine Neuron Diversity: Recent Advances and Current Challenges in Human Stem Cell Models and Single Cell Sequencing.	Fiorenzano A et al.	—	2021	→
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids.	Sen D et al.	—	2021	→
Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids.	Hoffmann PC et al.	—	2021	→
Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.	Forro C et al.	—	2021	→
Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells.	Koike H et al.	—	2021	→
Engineering organoids.	Hofer M et al.	—	2021	→
Evolutionary conservation and divergence of the human brain transcriptome.	Pembroke WG et al.	—	2021	→
Evolving features of human cortical development and the emerging roles of non-coding RNAs in neural progenitor cell diversity and function.	Prodromidou K et al.	—	2021	→
Expanding the boundaries of synthetic development.	Martyn I et al.	—	2021	→
Experimental Models to Study COVID-19 Effect in Stem Cells.	Chugh RM et al.	—	2021	→
From Brain Organoids to Networking Assembloids: Implications for Neuroendocrinology and Stress Medicine.	Makrygianni EA et al.	—	2021	→
From iPS Cells to Rodents and Nonhuman Primates: Filling Gaps in Modeling Parkinson's Disease.	Outeiro TF et al.	—	2021	→
Functional Characterization of Human Pluripotent Stem Cell-Derived Models of the Brain with Microelectrode Arrays.	Pelkonen A et al.	—	2021	→
Genome Editing in iPSC-Based Neural Systems: From Disease Models to Future Therapeutic Strategies.	McTague A et al.	—	2021	→
Growing Glia: Cultivating Human Stem Cell Models of Gliogenesis in Health and Disease.	Lanjewar SN et al.	—	2021	→
How well do brain organoids capture your brain?	Kim J et al.	—	2021	→
Human brain organoids assemble functionally integrated bilateral optic vesicles.	Gabriel E et al.	—	2021	→
Human mini-brain models.	Tan HY et al.	—	2021	→
Human neural organoids: Models for developmental neurobiology and disease.	Guy B et al.	—	2021	→
Human pluripotent stem cell-derived brain organoids as in vitro models for studying neural disorders and cancer.	Luo J et al.	—	2021	→
Identification of neural oscillations and epileptiform changes in human brain organoids.	Samarasinghe RA et al.	—	2021	→
Induced Pluripotent Stem Cells in Psychiatry: An Overview and Critical Perspective.	De Los Angeles A et al.	—	2021	→
In Vitro Recapitulation of Neuropsychiatric Disorders with Pluripotent Stem Cells-Derived Brain Organoids.	Gulimiheranmu M et al.	—	2021	→
iPSC-Derived Organoids as Therapeutic Models in Regenerative Medicine and Oncology.	Turhan AG et al.	—	2021	→
jSRC: a flexible and accurate joint learning algorithm for clustering of single-cell RNA-sequencing data.	Wu W et al.	—	2021	→
Long-term maturation of human cortical organoids matches key early postnatal transitions.	Gordon A et al.	—	2021	→
Methodologies for Generating Brain Organoids to Model Viral Pathogenesis in the CNS.	Hopkins HK et al.	—	2021	→
Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids.	Cho AN et al.	—	2021	→
Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids.	Fagerlund I et al.	—	2021	→
Mind the translational gap: using iPS cell models to bridge from genetic discoveries to perturbed pathways and therapeutic targets.	Pintacuda G et al.	—	2021	→
Modeling brain development and diseases with human cerebral organoids.	Shi Y et al.	—	2021	→
Modeling human neuronal migration deficits in 3D.	Reiner O et al.	—	2021	→
Modeling Neurodevelopmental and Neuropsychiatric Diseases with Astrocytes Derived from Human-Induced Pluripotent Stem Cells.	Ren B et al.	—	2021	→
Modeling neurodevelopment in a dish with pluripotent stem cells.	Imaizumi K et al.	—	2021	→
Modeling neurological disorders using brain organoids.	Zhang DY et al.	—	2021	→
Modeling of Hypoxic Brain Injury through 3D Human Neural Organoids.	Kim MS et al.	—	2021	→
Modeling Rett Syndrome with Human Pluripotent Stem Cells: Mechanistic Outcomes and Future Clinical Perspectives.	Gomes AR et al.	—	2021	→
Modeling Somatic Mutations Associated With Neurodevelopmental Disorders in Human Brain Organoids.	Deb BK et al.	—	2021	→
Modelling neurodegenerative disease using brain organoids.	Wray S	—	2021	→
Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.	Ciarpella F et al.	—	2021	→
Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration.	Bajaj S et al.	—	2021	→
Neurotropic Effects of SARS-CoV-2 Modeled by the Human Brain Organoids.	Ramani A et al.	—	2021	→
Next-Generation Human Cerebral Organoids as Powerful Tools To Advance NeuroHIV Research.	Premeaux TA et al.	—	2021	→
Novel <i>in vitro</i> Experimental Approaches to Study Myelination and Remyelination in the Central Nervous System.	Marangon D et al.	—	2021	→
Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells.	Bodnar B et al.	—	2021	→
Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease.	Galichet C et al.	—	2021	→
Optimization of cerebral organoids: a more qualified model for Alzheimer's disease research.	Bi FC et al.	—	2021	→
Organoids in Tissue Transplantation.	Sağraç D et al.	—	2021	→
Organoid technology: Current standing and future perspectives.	Shariati L et al.	—	2021	→
Patient-Derived Induced Pluripotent Stem Cells (iPSCs) and Cerebral Organoids for Drug Screening and Development in Autism Spectrum Disorder: Opportunities and Challenges.	Villa C et al.	—	2021	→
Patient-derived iPSC modeling of rare neurodevelopmental disorders: Molecular pathophysiology and prospective therapies.	Sabitha KR et al.	—	2021	→
Patterning of interconnected human brain spheroids.	Kim JJ et al.	—	2021	→
Potential ethical problems with human cerebral organoids: Consciousness and moral status of future brains in a dish.	Lavazza A	—	2021	→
Primate cell fusion disentangles gene regulatory divergence in neurodevelopment.	Agoglia RM et al.	—	2021	→
Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells.	Sivitilli A et al.	—	2021	→
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids.	Jourdon A et al.	—	2021	→
Publicly Available hiPSC Lines with Extreme Polygenic Risk Scores for Modeling Schizophrenia.	Dobrindt K et al.	—	2021	→
Rapid prototyping of cell culture microdevices using parylene-coated 3D prints.	O'Grady BJ et al.	—	2021	→
Recent advances in gene therapy for neurodevelopmental disorders with epilepsy.	Turner TJ et al.	—	2021	→
Recent Trends and Perspectives in Cerebral Organoids Imaging and Analysis.	Brémond Martin C et al.	—	2021	→
Regional specification and complementation with non-neuroectodermal cells in human brain organoids.	Tanaka Y et al.	—	2021	→
Resolving organoid brain region identities by mapping single-cell genomic data to reference atlases.	Fleck JS et al.	—	2021	→
Rethinking organoid technology through bioengineering.	Garreta E et al.	—	2021	→
Role of SHH in Patterning Human Pluripotent Cells towards Ventral Forebrain Fates.	Brady MV et al.	—	2021	→
SARS-CoV-2 targets glial cells in human cortical organoids.	McMahon CL et al.	—	2021	→
Screening Platforms for Genetic Epilepsies-Zebrafish, iPSC-Derived Neurons, and Organoids.	Shcheglovitov A et al.	—	2021	→
Single-cell transcriptomic analysis reveals key immune cell phenotypes in the lungs of patients with asthma exacerbation.	Li H et al.	—	2021	→
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids.	Fiorenzano A et al.	—	2021	→
Strategies Using Gelatin Microparticles for Regenerative Therapy and Drug Screening Applications.	Nii T	—	2021	→
Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction.	Golan M et al.	—	2021	→
Synaptic Hyaluronan Synthesis and CD44-Mediated Signaling Coordinate Neural Circuit Development.	Wilson ES et al.	—	2021	→
Taming human brain organoids one cell at a time.	Atamian A et al.	—	2021	→
Targeting α-Synuclein in Parkinson's Disease by Induced Pluripotent Stem Cell Models.	Spathopoulou A et al.	—	2021	→
The Age of Brain Organoids: Tailoring Cell Identity and Functionality for Normal Brain Development and Disease Modeling.	Porciúncula LO et al.	—	2021	→
The Gut-Brain Axis in Inflammatory Bowel Disease-Current and Future Perspectives.	Günther C et al.	—	2021	→
The Impact of Oxygen Availability and Multilineage Communication on Organoid Maturation.	Wörsdörfer P et al.	—	2021	→
The multiple facets of Cajal-Retzius neurons.	Causeret F et al.	—	2021	→
The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update.	Azar J et al.	—	2021	→
Three-dimensional in vitro tissue culture models of brain organoids.	Gong J et al.	—	2021	→
Three-dimensional, multifunctional neural interfaces for cortical spheroids and engineered assembloids.	Park Y et al.	—	2021	→
Tissue clearing and 3D imaging in developmental biology.	Vieites-Prado A et al.	—	2021	→
Toward Studying Cognition in a Dish.	Rouleau N et al.	—	2021	→
Toward three-dimensional <i>in vitro</i> models to study neurovascular unit functions in health and disease.	Caffrey TM et al.	—	2021	→
Transcriptome Dynamics of Human Neuronal Differentiation From iPSC.	Kuruş M et al.	—	2021	→
Trends and challenges in modeling glioma using 3D human brain organoids.	Mariappan A et al.	—	2021	→
Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach.	Raj V et al.	—	2021	→
Unraveling Human Brain Development and Evolution Using Organoid Models.	Fernandes S et al.	—	2021	→
Using iPSC Models to Understand the Role of Estrogen in Neuron-Glia Interactions in Schizophrenia and Bipolar Disorder.	Reis de Assis D et al.	—	2021	→
Using organoids to study human brain development and evolution.	Chan WK et al.	—	2021	→
Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.	Zhuang P et al.	—	2021	→
Vascularization of human brain organoids.	Matsui TK et al.	—	2021	→
WWOX-Related Neurodevelopmental Disorders: Models and Future Perspectives.	Steinberg DJ et al.	—	2021	→
3D Brain Organoids: Studying Brain Development and Disease Outside the Embryo.	Velasco S et al.	—	2020	→
Advanced Materials to Enhance Central Nervous System Tissue Modeling and Cell Therapy.	Muckom RJ et al.	—	2020	→
A Light-Inducible Hedgehog Signaling Activator Modulates Proliferation and Differentiation of Neural Cells.	Misawa R et al.	—	2020	→
A mouse model of Timothy syndrome exhibits altered social competitive dominance and inhibitory neuron development.	Horigane SI et al.	—	2020	→
A novel recombinant cccDNA-based mouse model with long term maintenance of rcccDNA and antigenemia.	Wu M et al.	—	2020	→
Application of Fused Organoid Models to Study Human Brain Development and Neural Disorders.	Chen A et al.	—	2020	→
Application of induced pluripotent stem cells in epilepsy.	Hirose S et al.	—	2020	→
A Primer on Human Brain Organoids for the Neurosurgeon.	Blue R et al.	—	2020	→
Autism spectrum disorder at the crossroad between genes and environment: contributions, convergences, and interactions in ASD developmental pathophysiology.	Cheroni C et al.	—	2020	→
Benchmarking pluripotent stem cell-derived organoid models.	De Los Angeles A et al.	—	2020	→
Biomaterials and Culture Systems for Development of Organoid and Organ-on-a-Chip Models.	D'Costa K et al.	—	2020	→
Biomimetic Materials and Their Utility in Modeling the 3-Dimensional Neural Environment.	Cembran A et al.	—	2020	→
Brain Organoids as Model Systems for Genetic Neurodevelopmental Disorders.	Baldassari S et al.	—	2020	→
Brain organoids for the study of human neurobiology at the interface of in vitro and in vivo.	Chiaradia I et al.	—	2020	→
Brain organoids: Human 3D models to investigate neuronal circuits assembly, function and dysfunction.	Tambalo M et al.	—	2020	→
Brain Organoids: Human Neurodevelopment in a Dish.	Benito-Kwiecinski S et al.	—	2020	→
BrainPhys neuronal medium optimized for imaging and optogenetics in vitro.	Zabolocki M et al.	—	2020	→
Building a Human Brain for Research.	Bitar M et al.	—	2020	→
Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models.	Gregory JA et al.	—	2020	→
Cerebral organoids as tools to identify the developmental roots of autism.	Chan WK et al.	—	2020	→
Challenges in Modeling Human Neural Circuit Formation via Brain Organoid Technology.	Matsui TK et al.	—	2020	→
Challenges in Physiological Phenotyping of hiPSC-Derived Neurons: From 2D Cultures to 3D Brain Organoids.	Mateos-Aparicio P et al.	—	2020	→
Chromatin accessibility dynamics in a model of human forebrain development.	Trevino AE et al.	—	2020	→
CNS organoids: an innovative tool for neurological disease modeling and drug neurotoxicity screening.	Chhibber T et al.	—	2020	→
CRISPR-based functional evaluation of schizophrenia risk variants.	Rajarajan P et al.	—	2020	→
Developmental excitation-inhibition imbalance underlying psychoses revealed by single-cell analyses of discordant twins-derived cerebral organoids.	Sawada T et al.	—	2020	→
Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids.	Zafeiriou MP et al.	—	2020	→
Do not keep it simple: recent advances in the generation of complex organoids.	Wörsdörfer P et al.	—	2020	→
Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological and Cellular Development.	Fair SR et al.	—	2020	→
Engineering Human Brain Organoids: From Basic Research to Tissue Regeneration.	Jeong HJ et al.	—	2020	→
Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease.	Gopal S et al.	—	2020	→
Exploring the mechanisms underlying excitation/inhibition imbalance in human iPSC-derived models of ASD.	Culotta L et al.	—	2020	→
Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks.	Dingle YL et al.	—	2020	→
Generation of cerebral cortical GABAergic interneurons from pluripotent stem cells.	Fitzgerald M et al.	—	2020	→
Generation of Functional Human 3D Cortico-Motor Assembloids.	Andersen J et al.	—	2020	→
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.	Miura Y et al.	—	2020	→
Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development.	Xiang Y et al.	—	2020	→
Genetic pathways involved in human speech disorders.	den Hoed J et al.	—	2020	→
Highly green fluorescent Nb<sub>2</sub>C MXene quantum dots.	Xu Q et al.	—	2020	→
Host-parasite interaction associated with major mental illness.	Kano SI et al.	—	2020	→
Human Brain Organoid Models of Developmental Epilepsies.	Nieto-Estévez V et al.	—	2020	→
Human cerebral organoids and consciousness: a double-edged sword.	Lavazza A	—	2020	→
Human-Derived Brain Models: Windows into Neuropsychiatric Disorders and Drug Therapies.	Papariello A et al.	—	2020	→
Human in vitro models for understanding mechanisms of autism spectrum disorder.	Gordon A et al.	—	2020	→
Human organoids: model systems for human biology and medicine.	Kim J et al.	—	2020	→
Human organoids to model the developing human neocortex in health and disease.	Khakipoor S et al.	—	2020	→
Human stem cell-based models for studying autism spectrum disorder-related neuronal dysfunction.	Cheffer A et al.	—	2020	→
Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding.	Salerno D et al.	—	2020	→
Increased Tau Expression Correlates with Neuronal Maturation in the Developing Human Cerebral Cortex.	Fiock KL et al.	—	2020	→
Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.	Lovett ML et al.	—	2020	→
Interneuron Types as Attractors and Controllers.	Fishell G et al.	—	2020	→
In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment.	Rosa F et al.	—	2020	→
iPSC modeling of rare pediatric disorders.	Freel BA et al.	—	2020	→
Joint learning dimension reduction and clustering of single-cell RNA-sequencing data.	Wu W et al.	—	2020	→
LHX6 is essential for the migration of human pluripotent stem cell-derived GABAergic interneurons.	Yuan F et al.	—	2020	→
Looking at neurodevelopment through a big data lens.	Briscoe J et al.	—	2020	→
Methadone Suppresses Neuronal Function and Maturation in Human Cortical Organoids.	Wu W et al.	—	2020	→
Microglia Play an Essential Role in Synapse Development and Neuron Maturation in Tissue-Engineered Neural Tissues.	Zhu H et al.	—	2020	→
Microphysiological Systems: Design, Fabrication, and Applications.	Wang K et al.	—	2020	→
miRNA-Based Rapid Differentiation of Purified Neurons from hPSCs Advancestowards Quick Screening for Neuronal Disease Phenotypes In Vitro.	Ishikawa M et al.	—	2020	→
Mitochondria under the spotlight: On the implications of mitochondrial dysfunction and its connectivity to neuropsychiatric disorders.	Zilocchi M et al.	—	2020	→
Modeling Alzheimer's disease with iPSC-derived brain cells.	Penney J et al.	—	2020	→
Modeling and Targeting Alzheimer's Disease With Organoids.	Papaspyropoulos A et al.	—	2020	→
Modeling Brain Disorders Using Induced Pluripotent Stem Cells.	Vadodaria KC et al.	—	2020	→
Modeling genetic epilepsies in a dish.	Niu W et al.	—	2020	→
Modeling HIV-1 neuropathogenesis using three-dimensional human brain organoids (hBORGs) with HIV-1 infected microglia.	Dos Reis RS et al.	—	2020	→
Modeling neural tube development by differentiation of human embryonic stem cells in a microfluidic WNT gradient.	Rifes P et al.	—	2020	→
Modeling neuropsychiatric disorders using human induced pluripotent stem cells.	Wang M et al.	—	2020	→
Modeling the complex genetic architectures of brain disease.	Fernando MB et al.	—	2020	→
Modeling traumatic brain injury with human brain organoids.	Jgamadze D et al.	—	2020	→
Modelling neurodegenerative diseases with 3D brain organoids.	Chang Y et al.	—	2020	→
Multi-lineage Human iPSC-Derived Platforms for Disease Modeling and Drug Discovery.	Sharma A et al.	—	2020	→
Multiparametric Assays for Accelerating Early Drug Discovery.	Herholt A et al.	—	2020	→
Multiscale 3D phenotyping of human cerebral organoids.	Albanese A et al.	—	2020	→
Negative Symptoms of Schizophrenia and Dopaminergic Transmission: Translational Models and Perspectives Opened by iPSC Techniques.	Collo G et al.	—	2020	→
Neuronal defects in a human cellular model of 22q11.2 deletion syndrome.	Khan TA et al.	—	2020	→
New frontiers in modeling tuberous sclerosis with human stem cell-derived neurons and brain organoids.	Blair JD et al.	—	2020	→
One-Stop Microfluidic Assembly of Human Brain Organoids To Model Prenatal Cannabis Exposure.	Ao Z et al.	—	2020	→
Opportunities and Challenges in Phenotypic Screening for Neurodegenerative Disease Research.	Brown DG et al.	—	2020	→
Organoid and Assembloid Technologies for Investigating Cellular Crosstalk in Human Brain Development and Disease.	Marton RM et al.	—	2020	→
Organoid and pluripotent stem cells in Parkinson's disease modeling: an expert view on their value to drug discovery.	Marotta N et al.	—	2020	→
Organoid based personalized medicine: from bench to bedside.	Li Y et al.	—	2020	→
Organoid technology for tissue engineering.	He J et al.	—	2020	→
Patient-Derived Midbrain Organoids to Explore the Molecular Basis of Parkinson's Disease.	Galet B et al.	—	2020	→
Probing disrupted neurodevelopment in autism using human stem cell-derived neurons and organoids: An outlook into future diagnostics and drug development.	Yang G et al.	—	2020	→
Probing infectious disease by single-cell RNA sequencing: Progresses and perspectives.	Luo G et al.	—	2020	→
Progress of Induced Pluripotent Stem Cell Technologies to Understand Genetic Epilepsy.	Sterlini B et al.	—	2020	→
Reality check for organoids in neuroscience.	Marx V	—	2020	→
Resolving Neurodevelopmental and Vision Disorders Using Organoid Single-Cell Multi-omics.	Brancati G et al.	—	2020	→
Retinal and Brain Organoids: Bridging the Gap Between <i>in vivo</i> Physiology and <i>in vitro</i> Micro-Physiology for the Study of Alzheimer's Diseases.	Brighi C et al.	—	2020	→
Reverse engineering human brain evolution using organoid models.	Mostajo-Radji MA et al.	—	2020	→
Review: <i>In vitro</i> Cell Platform for Understanding Developmental Toxicity.	Xie J et al.	—	2020	→
Review of Artificial Intelligence Applications and Algorithms for Brain Organoid Research.	Badai J et al.	—	2020	→
SARS-CoV-2 targets neurons of 3D human brain organoids.	Ramani A et al.	—	2020	→
SCN2A channelopathies in the autism spectrum of neuropsychiatric disorders: a role for pluripotent stem cells?	Kruth KA et al.	—	2020	→
Self-Organizing 3D Human Trunk Neuromuscular Organoids.	Faustino Martins JM et al.	—	2020	→
Shifting Developmental Trajectories During Critical Periods of Brain Formation.	Dehorter N et al.	—	2020	→
Simplified Brain Organoids for Rapid and Robust Modeling of Brain Disease.	Ha J et al.	—	2020	→
Stem cells: A path towards improved epilepsy therapies.	Lybrand ZR et al.	—	2020	→
Stem Cells for Improving the Treatment of Neurodevelopmental Disorders.	Donegan JJ et al.	—	2020	→
Studying Human Neurodevelopment and Diseases Using 3D Brain Organoids.	Tian A et al.	—	2020	→
Surface-Functionalized Self-Standing Microdevices Exhibit Predictive Localization and Seamless Integration in 3D Neural Spheroids.	Lecomte A et al.	—	2020	→
Synthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain.	Tanaka Y et al.	—	2020	→
The abiding relevance of mouse models of rare mutations to psychiatric neuroscience and therapeutics.	Gogos JA et al.	—	2020	→
The Implementation of the Three Rs in Regulatory Toxicity and Biosafety Assessment: The Indian Perspective.	Pant AB	—	2020	→
The Use of Patient-Derived Induced Pluripotent Stem Cells for Alzheimer's Disease Modeling.	Lee C et al.	—	2020	→
Three-Dimensional Models for Studying Neurodegenerative and Neurodevelopmental Diseases.	Tsaridou S et al.	—	2020	→
Three-Dimensional Printed Stamps for the Fabrication of Patterned Microwells and High-Throughput Production of Homogeneous Cell Spheroids.	Gonzalez-Fernandez T et al.	—	2020	→
Tissue clearing and its applications in neuroscience.	Ueda HR et al.	—	2020	→
Toward Spatial Identities in Human Brain Organoids-on-Chip Induced by Morphogen-Soaked Beads.	Ben-Reuven L et al.	—	2020	→
Translating Embryogenesis to Generate Organoids: Novel Approaches to Personalized Medicine.	Sahu S et al.	—	2020	→
Uncovering cell biology in the third dimension.	Robertson GL et al.	—	2020	→
Upgrading the Physiological Relevance of Human Brain Organoids.	Del Dosso A et al.	—	2020	→
Using brain organoids to study human neurodevelopment, evolution and disease.	Kyrousi C et al.	—	2020	→
Using Two- and Three-Dimensional Human iPSC Culture Systems to Model Psychiatric Disorders.	Christian KM et al.	—	2020	→
3D bioprinting models of neural tissues: The current state of the field and future directions.	de la Vega L et al.	—	2019	→
Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome.	Panagiotakos G et al.	—	2019	→
Addressing Patient Specificity in the Engineering of Tumor Models.	Bray LJ et al.	—	2019	→
A framework for the investigation of rare genetic disorders in neuropsychiatry.	Sanders SJ et al.	—	2019	→
All Together Now: Modeling the Interaction of Neural With Non-neural Systems Using Organoid Models.	Chukwurah E et al.	—	2019	→
Analysis of Synapses in Cerebral Organoids.	Yakoub AM et al.	—	2019	→
An atlas of nano-enabled neural interfaces.	Acarón Ledesma H et al.	—	2019	→
Applications of Human Brain Organoids to Clinical Problems.	Chen HI et al.	—	2019	→
Assembling human brain organoids.	Paşca SP	—	2019	→
Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues.	Song L et al.	—	2019	→
Automated four-dimensional long term imaging enables single cell tracking within organotypic brain slices to study neurodevelopment and degeneration.	Linsley JW et al.	—	2019	→
Biomaterials and Advanced Biofabrication Techniques in hiPSCs Based Neuromyopathic Disease Modeling.	Sun J et al.	—	2019	→
Brain Organoids-A Bottom-Up Approach for Studying Human Neurodevelopment.	Karzbrun E et al.	—	2019	→
Brain organoids: advances, applications and challenges.	Qian X et al.	—	2019	→
Brain Organoids: A New, Transformative Investigational Tool for Neuroscience Research.	Vaez Ghaemi R et al.	—	2019	→
Brain organoids: a next step for humanized Alzheimer's disease models?	Gerakis Y et al.	—	2019	→
Brain Organoids as Tools for Modeling Human Neurodevelopmental Disorders.	Adams JW et al.	—	2019	→
Building brains: using brain organoids to study neural development and disease.	Lyon L	—	2019	→
Bundled Three-Dimensional Human Axon Tracts Derived from Brain Organoids.	Cullen DK et al.	—	2019	→
Calcium signalling: a key regulator of neuronal migration.	Horigane SI et al.	—	2019	→
Cell death assays for neurodegenerative disease drug discovery.	Linsley JW et al.	—	2019	→
Cell diversity in the human cerebral cortex: from the embryo to brain organoids.	Arlotta P et al.	—	2019	→
Cell migration promotes dynamic cellular interactions to control cerebral cortex morphogenesis.	Silva CG et al.	—	2019	→
Cerebral Cortex Generated from Pluripotent Stem Cells to Model Corticogenesis and Rebuild Cortical Circuits: In Vitro Veritas?	Varrault A et al.	—	2019	→
Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output.	Giandomenico SL et al.	—	2019	→
Cerebral organoids exhibit mature neurons and astrocytes and recapitulate electrophysiological activity of the human brain.	Yakoub AM	—	2019	→
Compartmentalized Devices as Tools for Investigation of Human Brain Network Dynamics.	Fantuzzo JA et al.	—	2019	→
Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development.	Trujillo CA et al.	—	2019	→
Construction of 3D in vitro models by bioprinting human pluripotent stem cells: Challenges and opportunities.	Salaris F et al.	—	2019	→
Contribution of induced pluripotent stem cell technologies to the understanding of cellular phenotypes in schizophrenia.	Balan S et al.	—	2019	→
Convergence of human cellular models and genetics to study neural stem cell signaling to enhance central nervous system regeneration and repair.	Julian D et al.	—	2019	→
Cortical Dysmaturation in Congenital Heart Disease.	Leonetti C et al.	—	2019	→
Cortical interneuron function in autism spectrum condition.	Lunden JW et al.	—	2019	→
Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops.	Czechowska K et al.	—	2019	→
Differential Effects of Extracellular Vesicles of Lineage-Specific Human Pluripotent Stem Cells on the Cellular Behaviors of Isogenic Cortical Spheroids.	Marzano M et al.	—	2019	→
Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures.	Marton RM et al.	—	2019	→
Disease modelling in human organoids.	Lancaster MA et al.	—	2019	→
Early Actions of Neurotransmitters During Cortex Development and Maturation of Reprogrammed Neurons.	Ojeda J et al.	—	2019	→
Engineered materials for organoid systems.	Kratochvil MJ et al.	—	2019	→
Engineering Stem Cell Self-organization to Build Better Organoids.	Brassard JA et al.	—	2019	→
Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells.	Song L et al.	—	2019	→
Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain-derived matrix.	Sood D et al.	—	2019	→
Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System.	Mattei C et al.	—	2019	→
Genetic Associations between Voltage-Gated Calcium Channels and Psychiatric Disorders.	Andrade A et al.	—	2019	→
Genetic Modification of Brain Organoids.	Fischer J et al.	—	2019	→
Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids.	Bejoy J et al.	—	2019	→
Getting to the Cores of Autism.	Iakoucheva LM et al.	—	2019	→
hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids.	Xiang Y et al.	—	2019	→
High-throughput single-cell transcriptomics on organoids.	Brazovskaja A et al.	—	2019	→
Human 3D cellular model of hypoxic brain injury of prematurity.	Pașca AM et al.	—	2019	→
Human brain development and its in vitro recapitulation.	Seto Y et al.	—	2019	→
Human brain development through the lens of cerebral organoid models.	Andrews MG et al.	—	2019	→
Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities.	Nascimento JM et al.	—	2019	→
Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis.	López-Tobón A et al.	—	2019	→
Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 2: Spheroids, Organoids, and Disease Modeling.	Eglen RM et al.	—	2019	→
Individual brain organoids reproducibly form cell diversity of the human cerebral cortex.	Velasco S et al.	—	2019	→
Inferring Regulatory Programs Governing Region Specificity of Neuroepithelial Stem Cells during Early Hindbrain and Spinal Cord Development.	Chasman D et al.	—	2019	→
iPSCs-Based Neural 3D Systems: A Multidimensional Approach for Disease Modeling and Drug Discovery.	Costamagna G et al.	—	2019	→
Liver organoids: from basic research to therapeutic applications.	Prior N et al.	—	2019	→
Microfluidics tubing as a synthesizer for ordered microgel networks.	Ma S	—	2019	→
Modeling Brain Somatic Mosaicism With Cerebral Organoids, Including a Note on Mutant Microglia.	Verheijen BM	—	2019	→
Modeling cell-cell interactions in the brain using cerebral organoids.	Oliveira B et al.	—	2019	→
Modeling Human Brain Circuitry Using Pluripotent Stem Cell Platforms.	Hartlaub AM et al.	—	2019	→
Modeling Psychiatric Diseases with Induced Pluripotent Stem Cells.	van Hugte E et al.	—	2019	→
Mouse vs man: Organoid models of brain development & disease.	Marshall JJ et al.	—	2019	→
Neural Lineage Differentiation From Pluripotent Stem Cells to Mimic Human Brain Tissues.	Hong YJ et al.	—	2019	→
Neuronal migration in the CNS during development and disease: insights from <i>in vivo</i> and <i>in vitro</i> models.	Buchsbaum IY et al.	—	2019	→
Non-contact monitoring of extra-cellular field potentials with a multi-electrode array.	Sharf T et al.	—	2019	→
One Step Into the Future: New iPSC Tools to Advance Research in Parkinson's Disease and Neurological Disorders.	Mohamed NV et al.	—	2019	→
Optogenetics in the Era of Cerebral Organoids.	Shiri Z et al.	—	2019	→
Organ-on-chip models: Implications in drug discovery and clinical applications.	Mittal R et al.	—	2019	→
Organs to Cells and Cells to Organoids: The Evolution of <i>in vitro</i> Central Nervous System Modelling.	Pacitti D et al.	—	2019	→
Oxidative DNA Damage Signalling in Neural Stem Cells in Alzheimer's Disease.	Kieroń M et al.	—	2019	→
Past, Present, and Future of Brain Organoid Technology.	Koo B et al.	—	2019	→
Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins.	Kim H et al.	—	2019	→
Pluripotent Stem Cells for Brain Repair: Protocols and Preclinical Applications in Cortical and Hippocampal Pathologies.	Alia C et al.	—	2019	→
Precisely controlling endogenous protein dosage in hPSCs and derivatives to model FOXG1 syndrome.	Zhu W et al.	—	2019	→
Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System.	Shum EY et al.	—	2019	→
Reliability of human cortical organoid generation.	Yoon SJ et al.	—	2019	→
Reply: Novel GABRA2 variants in epileptic encephalopathy and intellectual disability with seizures.	Jenkins A et al.	—	2019	→
Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids.	Sakaguchi H et al.	—	2019	→
Single-cell multimodal transcriptomics to study neuronal diversity in human stem cell-derived brain tissue and organoid models.	van den Hurk M et al.	—	2019	→
Single-Cell Multi-omic Integration Compares and Contrasts Features of Brain Cell Identity.	Welch JD et al.	—	2019	→
Species-Specific miRNAs in Human Brain Development and Disease.	Prodromidou K et al.	—	2019	→
Specification of positional identity in forebrain organoids.	Cederquist GY et al.	—	2019	→
Stalled developmental programs at the root of pediatric brain tumors.	Jessa S et al.	—	2019	→
Synergistic effects of common schizophrenia risk variants.	Schrode N et al.	—	2019	→
The diversity of GABAergic neurons and neural communication elements.	Huang ZJ et al.	—	2019	→
The Emergence of Stem Cell-Based Brain Organoids: Trends and Challenges.	Gopalakrishnan J	—	2019	→
The Emerging Neurobiology of Bipolar Disorder.	Harrison PJ et al.	—	2019	→
The Ethics of Cerebral Organoid Research: Being Conscious of Consciousness.	Sawai T et al.	—	2019	→
The use of iPSC technology for modeling Autism Spectrum Disorders.	Russo FB et al.	—	2019	→
The Use of Pluripotent Stem Cell-Derived Organoids to Study Extracellular Matrix Development during Neural Degeneration.	Yan Y et al.	—	2019	→
Transplantation of Human Brain Organoids: Revisiting the Science and Ethics of Brain Chimeras.	Chen HI et al.	—	2019	→
Valid Post-clustering Differential Analysis for Single-Cell RNA-Seq.	Zhang JM et al.	—	2019	→
2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling.	Centeno EGZ et al.	—	2018	→
An interpretable framework for clustering single-cell RNA-Seq datasets.	Zhang JM et al.	—	2018	→
An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids.	Karzbrun E et al.	—	2018	→
Autism spectrum disorders: Challenges and perspectives.	Muotri AR	—	2018	→
Bench to bedside: Current advances in regenerative medicine.	Clarke G et al.	—	2018	→
Brain Organoids and the Study of Neurodevelopment.	Trujillo CA et al.	—	2018	→
Brain organoids as models to study human neocortex development and evolution.	Heide M et al.	—	2018	→
Building Models of Brain Disorders with Three-Dimensional Organoids.	Amin ND et al.	—	2018	→
Central Nervous System Responses to Simulated Galactic Cosmic Rays.	Cekanaviciute E et al.	—	2018	→
Cerebral organoids: ethical issues and consciousness assessment.	Lavazza A et al.	—	2018	→
Chromatin Remodeling Proteins in Epilepsy: Lessons From <i>CHD2</i>-Associated Epilepsy.	Lamar KJ et al.	—	2018	→
Cortical organoids: why all this hype?	Marsoner F et al.	—	2018	→
Directed fusion of cardiac spheroids into larger heterocellular microtissues enables investigation of cardiac action potential propagation via cardiac fibroblasts.	Kim TY et al.	—	2018	→
Drug screening for human genetic diseases using iPSC models.	Elitt MS et al.	—	2018	→
Dynamism of an Astrocyte In Vivo: Perspectives on Identity and Function.	Poskanzer KE et al.	—	2018	→
Epigenetics and cerebral organoids: promising directions in autism spectrum disorders.	Forsberg SL et al.	—	2018	→
Exploring landscapes of brain morphogenesis with organoids.	Jabaudon D et al.	—	2018	→
Generation and assembly of human brain region-specific three-dimensional cultures.	Sloan SA et al.	—	2018	→
Generation and Fusion of Human Cortical and Medial Ganglionic Eminence Brain Organoids.	Xiang Y et al.	—	2018	→
Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.	Qian X et al.	—	2018	→
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells.	Krefft O et al.	—	2018	→
Genetics of Alcohol Use Disorder: A Role for Induced Pluripotent Stem Cells?	Prytkova I et al.	—	2018	→
Genomics in neurodevelopmental disorders: an avenue to personalized medicine.	Tărlungeanu DC et al.	—	2018	→
Human Cortical Neuron Generation Using Cell Reprogramming: A Review of Recent Advances.	McCaughey-Chapman A et al.	—	2018	→
Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.	Sances S et al.	—	2018	→
Human Models Are Needed for Studying Human Neurodevelopmental Disorders.	Zhao X et al.	—	2018	→
Human Neurospheroid Arrays for In Vitro Studies of Alzheimer's Disease.	Jorfi M et al.	—	2018	→
Induced pluripotent stem cells as a tool to study brain circuits in autism-related disorders.	Vitrac A et al.	—	2018	→
Induction of myelinating oligodendrocytes in human cortical spheroids.	Madhavan M et al.	—	2018	→
In Vivo Imaging of Single Mammalian Cells in Development and Disease.	White MD et al.	—	2018	→
Modeling Neurodegenerative Microenvironment Using Cortical Organoids Derived from Human Stem Cells.	Yan Y et al.	—	2018	→
Modeling Neurological Diseases With Human Brain Organoids.	Wang H	—	2018	→
Modeling Pediatric Epilepsy Through iPSC-Based Technologies.	Simkin D et al.	—	2018	→
Modeling rare diseases with induced pluripotent stem cell technology.	Anderson RH et al.	—	2018	→
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.	Song L et al.	—	2018	→
Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.	Pothayee N et al.	—	2018	→
Neural stem cells and epilepsy: functional roles and disease-in-a-dish models.	Thodeson DM et al.	—	2018	→
Neuroprotective Activities of Heparin, Heparinase III, and Hyaluronic Acid on the A<i>β</i>42-Treated Forebrain Spheroids Derived from Human Stem Cells.	Bejoy J et al.	—	2018	→
Open chromatin dynamics reveals stage-specific transcriptional networks in hiPSC-based neurodevelopmental model.	Zhang S et al.	—	2018	→
Organoids for Modeling Genetic Diseases.	Perez-Lanzon M et al.	—	2018	→
Organoids required! A new path to understanding human brain development and disease.	Arlotta P	—	2018	→
Progress in Understanding and Treating SCN2A-Mediated Disorders.	Sanders SJ et al.	—	2018	→
Psychiatry in a Dish: Stem Cells and Brain Organoids Modeling Autism Spectrum Disorders.	Ilieva M et al.	—	2018	→
Review: Synthetic scaffolds to control the biochemical, mechanical, and geometrical environment of stem cell-derived brain organoids.	Oksdath M et al.	—	2018	→
Self-Organized Nanostructure Modified Microelectrode for Sensitive Electrochemical Glutamate Detection in Stem Cells-Derived Brain Organoids.	Nasr B et al.	—	2018	→
ShinyCortex: Exploring Single-Cell Transcriptome Data From the Developing Human Cortex.	Kageyama J et al.	—	2018	→
Single-cell genomics to guide human stem cell and tissue engineering.	Camp JG et al.	—	2018	→
Single cell RNA sequencing of stem cell-derived retinal ganglion cells.	Daniszewski M et al.	—	2018	→
Stem Cells, Genome Editing, and the Path to Translational Medicine.	Soldner F et al.	—	2018	→
Studying and modulating schizophrenia-associated dysfunctions of oligodendrocytes with patient-specific cell systems.	Raabe FJ et al.	—	2018	→
Studying the Brain in a Dish: 3D Cell Culture Models of Human Brain Development and Disease.	Brown J et al.	—	2018	→
Synaptic dysfunction in neurodegenerative and neurodevelopmental diseases: an overview of induced pluripotent stem-cell-based disease models.	Taoufik E et al.	—	2018	→
The Emerging Neurobiology of Bipolar Disorder.	Harrison PJ et al.	—	2018	→
The ethics of experimenting with human brain tissue.	Farahany NA et al.	—	2018	→
The Feasibility of Encapsulated Embryonic Medullary Reticular Cells to Grow and Differentiate Into Neurons in Functionalized Gelatin-Based Hydrogels.	Magariños AM et al.	—	2018	→
The rise of three-dimensional human brain cultures.	Pașca SP	—	2018	→
Three-Dimensional Models of the Human Brain Development and Diseases.	Jorfi M et al.	—	2018	→
Tissue Engineering and Regenerative Medicine 2017: A Year in Review.	Park KM et al.	—	2018	→
Translational potential of human brain organoids.	Sun AX et al.	—	2018	→
Using mouse transgenic and human stem cell technologies to model genetic mutations associated with schizophrenia and autism.	St Clair D et al.	—	2018	→
Vascularized microfluidic organ-chips for drug screening, disease models and tissue engineering.	Osaki T et al.	—	2018	→
Viral Strategies for Targeting the Central and Peripheral Nervous Systems.	Bedbrook CN et al.	—	2018	→
3D Bioprinting and In Vitro Cardiovascular Tissue Modeling.	Jang J	—	2017	→
A Little Bit of Guidance: Mini Brains on Their Route to Adolescence.	Koch P et al.	—	2017	→
Compartmentalized embryoid body culture for induction of spatially patterned differentiation.	Kaneda S et al.	—	2017	→
Concise Review: Induced Pluripotent Stem Cell Models for Neuropsychiatric Diseases.	Adegbola A et al.	—	2017	→
Fused cerebral organoids model interactions between brain regions.	Bagley JA et al.	—	2017	→
Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration.	Xiang Y et al.	—	2017	→
Generating CNS organoids from human induced pluripotent stem cells for modeling neurological disorders.	Brawner AT et al.	—	2017	→
Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.	Sloan SA et al.	—	2017	→
Human development: Advances in mini-brain technology.	Camp JG et al.	—	2017	→
Lab-grown mini-brains upgraded.	Yang L et al.	—	2017	→
Mechanisms of radial glia progenitor cell lineage progression.	Beattie R et al.	—	2017	→
Modeling human brain development.	Chung K	—	2017	→
Modeling schizophrenia pathogenesis using patient-derived induced pluripotent stem cells (iPSCs).	Noh H et al.	—	2017	→
Organoids: a better in vitro model.	Foley KE	—	2017	→
Prospects for Modeling Abnormal Neuronal Function in Schizophrenia Using Human Induced Pluripotent Stem Cells.	Prytkova I et al.	—	2017	→
Radial glia in the ventral telencephalon.	Turrero García M et al.	—	2017	→
Recent advances in modelling of cerebellar ataxia using induced pluripotent stem cells.	Wong MMK et al.	—	2017	→
Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes.	Krencik R et al.	—	2017	→
The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas.	Ecker JR et al.	—	2017	→
The use of brain organoids to investigate neural development and disease.	Di Lullo E et al.	—	2017	→
Wnt/β-catenin signaling during early vertebrate neural development.	Brafman D et al.	—	2017	→
You Have Brains in Your Head, You Have Organoids in Your Dish, You Can Steer Yourself in any Direction You Wish.	Thodeson DM et al.	—	2017	→