Integrating mRNA and miRNA Weighted Gene Co-Expression Networks with eQTLs in the Nucleus Accumbens of Subjects with Alcohol Dependence.

This figure consists of two cluster dendrograms (A and B) showing Weighted Gene Co-expression Network Analysis (WGCNA) for mRNA and miRNA, respectively. Both panels feature a vertical axis labeled "Height" and a corresponding horizontal color bar at the bottom indicating module assignments. Panel A shows 4,571 transcripts partitioned into 24 modules, while Panel B shows 240 miRNAs partitioned into 12 modules.

This figure consists of two heatmaps (A and B) showing Pearson correlation coefficients ($r$) and p-values between gene module eigengenes (MEs) and various clinical traits. The x-axes list traits including AD case-status (Class), brain pH, PMI, Age, RIN, and smoking status, while the y-axes list specific mRNA (A) and miRNA (B) modules. Color coding indicates the strength and direction of the correlation, with red representing positive and blue representing negative correlations.

This figure consists of two sets of scatter plots (A and B) showing the relationship between Module Membership (x-axis) and Gene Significance (y-axis) for various mRNA and miRNA modules correlated with AD case-status. Each plot includes a linear regression line, a correlation coefficient (cor), and a p-value, indicating a positive correlation between intramodular connectivity and expression correlation to AD status. Panel A displays six mRNA modules (Green, Grey60, Salmon, Yellow, Turquoise, Pink), while Panel B displays three miRNA modules (Yellow, Brown, Blue).

This is a heatmap showing Pearson correlation coefficients ($r$) and p-values between significant mRNA module eigengenes (rows: METurquoise, MEyellow, MEgrey60, MEpink, MEgreen, MEsalmon) and miRNA module eigengenes (columns: blue, brown, yellow). The color scale ranges from -1 (blue, negative correlation) to 1 (red, positive correlation). The strongest positive correlation is observed between miRNA MEbrown and mRNA MEgrey60 ($r = 0.75, p = 2.6\text{e-}07$), while strong negative correlations are visible between miRNA MEblue and mRNA MEgrey60 ($r = -0.56$) and miRNA MEbrown and mRNA METurquoise ($r = -0.56$).

This box plot shows the expression levels of the GAD1 gene across three genotypes: homozygote major (11, red), heterozygote (12, green), and homozygote minor (22, blue). The y-axis represents expression values, ranging from approximately 9.5 to 12.5. Expression is highest and most consistent in the 11 and 12 groups, while the 22 group shows a marked decrease in median expression and greater variability.

This figure consists of two box plots (A and B) showing the expression levels of hsa-miR-134-5p and hsa-miR-370-3p across three genotypes of rs11626307 (11, 21, and 22). In both plots, expression levels decrease progressively from genotype 11 (red) to 21 (green) to 22 (blue). The y-axes represent expression values, while the x-axes label the specific genotypes.