Gene expression changes in the nucleus accumbens of alcohol-preferring rats following chronic ethanol consumption.
paper
Cited
Public
- Authors
- Bell, Richard L; Kimpel, Mark W; McClintick, Jeanette N; Strother, Wendy N; Carr, Lucinda G; Liang, Tiebing; Rodd, Zachary A; Mayfield, R Dayne; Edenberg, Howard J; McBride, William J
- Year
- 2009
- Journal
- Pharmacology, biochemistry, and behavior
- PMID
- 19666046
- DOI
- 10.1016/j.pbb.2009.07.019
- PMCID
- PMC2771758
The objective of this study was to determine the effects of binge-like alcohol drinking on gene expression changes in the nucleus accumbens (ACB) of alcohol-preferring (P) rats. Adult male P rats were given ethanol under multiple scheduled access (MSA; three 1-h dark cycle sessions/day) conditions for 8 weeks. For comparison purposes, a second ethanol drinking group was given continuous/daily alcohol access (CA; 24h/day). A third group was ethanol-naรฏve (W group). Average ethanol intakes for the CA and MSA groups were approximately 9.5 and 6.5 g/kg/day, respectively. Fifteen hours after the last drinking episode, rats were euthanized, the brains extracted, and the ACB dissected. RNA was extracted and purified for microarray analysis. The only significant differences were between the CA and W groups (p<0.01; Storey false discovery rate=0.15); there were 374 differences in named genes between these 2 groups. There were 20 significant Gene Ontology (GO) categories, which included negative regulation of protein kinase activity, anti-apoptosis, and regulation of G-protein coupled receptor signaling. Ingenuity analysis indicated a network of transcription factors, involving oncogenes (Fos, Jun, Junb had higher expression in the ACB of the CA group), suggesting increased neuronal activity. There were 43 genes located within rat QTLs for alcohol consumption and preference; 4 of these genes (Tgfa, Hspa5, Mtus1 and Creb3l2) are involved in anti-apoptosis and increased transcription, suggesting that they may be contributing to cellular protection and maintaining high alcohol intakes. Overall, these findings suggest that chronic CA drinking results in genomic changes that can be observed during the early acute phase of ethanol withdrawal. Conversely, chronic MSA drinking, with its associated protracted withdrawal periods, results in genomic changes that may be masked by tight regulation of these genes following repeated experiences of ethanol withdrawal.
No figures extracted from this document.
1โ20 of 52
Next โ
No entities extracted from this document yet.
No uploaded files.
Not in any collection.
In this knowledge base
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Cocaine-induced gene regulation in D1 and D2 neuronal ensembles of the nucleus accumbens. | Mews P et al. | โ | 2025 | โ |
| Integrated single-cell multiomic profiling of caudate nucleus suggests key mechanisms in alcohol use disorder. | Green NC et al. | โ | 2025 | โ |
| Adaptations in glutathione-based redox protein signaling pathways and alcohol drinking across species. | Womersley JS et al. | โ | 2024 | โ |
| Effects of the Phosphodiesterase 10A Inhibitor MR1916 on Alcohol Self-Administration and Striatal Gene Expression in Post-Chronic Intermittent Ethanol-Exposed Rats. | Bertotto LB et al. | โ | 2024 | โ |
| Identification of novel genetic loci and candidate genes for progressive ethanol consumption in diversity outbred mice. | Mignogna KM et al. | โ | 2024 | โ |
| K<sub>v</sub>7 channel opener retigabine reduces self-administration of cocaine but not sucrose in rats. | Urena ES et al. | โ | 2024 | โ |
| Modeling methamphetamine use disorder and relapse in animals: short- and long-term epigenetic, transcriptional., and biochemical consequences in the rat brain. | Elhadi K et al. | โ | 2023 | โ |
| Blood and brain gene expression signatures of chronic intermittent ethanol consumption in mice. | Ferguson LB et al. | โ | 2022 | โ |
| Dopamine Release in Nucleus Accumbens Is under Tonic Inhibition by Adenosine A<sub>1</sub> Receptors Regulated by Astrocytic ENT1 and Dysregulated by Ethanol. | Roberts BM et al. | โ | 2022 | โ |
| Effect of chronic intermittent ethanol vapor exposure on RNA content of brain-derived extracellular vesicles. | Baratta AM et al. | โ | 2022 | โ |
| Genetic Mapping of Progressive Ethanol Consumption in the Diversity Outbred Mouse | Tatom Z et al. | โ | 2022 | โ |
| Alcohol Dependence in Rats Is Associated with Global Changes in Gene Expression in the Central Amygdala. | Kisby BR et al. | โ | 2021 | โ |
| Alcohol use disorder causes global changes in splicing in the human brain. | Van Booven D et al. | โ | 2021 | โ |
| Cell-type specialization is encoded by specific chromatin topologies. | Winick-Ng W et al. | โ | 2021 | โ |
| Curation of over 10โ000 transcriptomic studies to enable data reuse. | Lim N et al. | โ | 2021 | โ |
| Differential regulation of alcohol consumption and reward by the transcriptional cofactor LMO4. | Maiya R et al. | โ | 2021 | โ |
| Genetic contributions to alcohol use disorder treatment outcomes: a genome-wide pharmacogenomics study. | Biernacka JM et al. | โ | 2021 | โ |
| The Role of DND1 in Cancers. | Zhang Y et al. | โ | 2021 | โ |
| Systems genetics analysis of the LXS recombinant inbred mouse strains:Genetic and molecular insights into acute ethanol tolerance. | Radcliffe RA et al. | โ | 2020 | โ |
| Type 1 equilibrative nucleoside transporter (ENT1) regulates sex-specific ethanol drinking during disruption of circadian rhythms. | Jia YF et al. | โ | 2020 | โ |
| Animal models of binge drinking, current challenges to improve face validity. | Jeanblanc J et al. | โ | 2019 | โ |
| Ethanol activates immune response in lymphoblastoid cells. | McClintick JN et al. | โ | 2019 | โ |
| Towards precision medicine for pain: diagnostic biomarkers and repurposed drugs. | Niculescu AB et al. | โ | 2019 | โ |
| Binge Ethanol Drinking Produces Sexually Divergent and Distinct Changes in Nucleus Accumbens Signaling Cascades and Pathways in Adult C57BL/6J Mice. | Finn DA et al. | โ | 2018 | โ |
| Gene expression changes in the ventral hippocampus and medial prefrontal cortex of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking. | McClintick JN et al. | โ | 2018 | โ |
| Persistent Neuroadaptations in the Expression of Genes Involved in Cholesterol Homeostasis Induced by Chronic, Voluntary Alcohol Intake in Rats. | Alsebaaly J et al. | โ | 2018 | โ |
| Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice. | Baker JA et al. | โ | 2017 | โ |
| Effects of selection for ethanol preference on gene expression in the nucleus accumbens of HS-CC mice. | Colville AM et al. | โ | 2017 | โ |
| Glutamate plasticity woven through the progression to alcohol use disorder: a multi-circuit perspective. | Hwa L et al. | โ | 2017 | โ |
| iTRAQ proteomic analysis of the hippocampus in a rat model of nicotine-induced conditioned place preference. | Zhu B et al. | โ | 2017 | โ |
| Rat animal models for screening medications to treat alcohol use disorders. | Bell RL et al. | โ | 2017 | โ |
| A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction. | Bell RL et al. | โ | 2016 | โ |
| Alcohol and tobacco consumption alter hypothalamic pituitary adrenal axis DNA methylation. | Dogan MV et al. | โ | 2016 | โ |
| Assessment of individual differences in the rat nucleus accumbens transcriptome following taste-heroin extended access. | Imperio CG et al. | โ | 2016 | โ |
| A Systems Approach Implicates a Brain Mitochondrial Oxidative Homeostasis Co-expression Network in Genetic Vulnerability to Alcohol Withdrawal. | Walter NA et al. | โ | 2016 | โ |
| Ethanol-Associated Changes in Glutamate Reward Neurocircuitry: A Minireview of Clinical and Preclinical Genetic Findings. | Bell RL et al. | โ | 2016 | โ |
| Inflexible ethanol intake: A putative link with the Lrrk2 pathway. | da Silva E Silva DA et al. | โ | 2016 | โ |
| Kv7 channels in the nucleus accumbens are altered by chronic drinking and are targets for reducing alcohol consumption. | McGuier NS et al. | โ | 2016 | โ |
| Profiling ethanol-targeted transcription factors in human carcinoma cell-derived embryoid bodies. | Mandal C et al. | โ | 2016 | โ |
| Reward devaluation and heroin escalation is associated with differential expression of CRF signaling genes. | McFalls AJ et al. | โ | 2016 | โ |
| RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in Ethanol-Treated Embryoid Bodies. | Mandal C et al. | โ | 2016 | โ |
| Rodent models and mechanisms of voluntary binge-like ethanol consumption: Examples, opportunities, and strategies for preclinical research. | Fritz BM et al. | โ | 2016 | โ |
| Alpha-melanocyte stimulating hormone modulates ethanol self-administration in posterior ventral tegmental area through melanocortin-4 receptors. | Shelkar GP et al. | โ | 2015 | โ |
| Commonalities and Distinctions Among Mechanisms of Addiction to Alcohol and Other Drugs. | Ozburn AR et al. | โ | 2015 | โ |
| Gene expression changes in serotonin, GABA-A receptors, neuropeptides and ion channels in the dorsal raphe nucleus of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking. | McClintick JN et al. | โ | 2015 | โ |
| Gene expression profile analysis of rat cerebellum under acute alcohol intoxication. | Zhang Y et al. | โ | 2015 | โ |
| Nicotine withdrawal increases stress-associated genes in the nucleus accumbens of female rats in a hormone-dependent manner. | Torres OV et al. | โ | 2015 | โ |
| Novel candidate genes for alcoholism--transcriptomic analysis of prefrontal medial cortex, hippocampus and nucleus accumbens of Warsaw alcohol-preferring and non-preferring rats. | Stankiewicz AM et al. | โ | 2015 | โ |
| Changes in gene expression within the extended amygdala following binge-like alcohol drinking by adolescent alcohol-preferring (P) rats. | McBride WJ et al. | โ | 2014 | โ |
| Dual-trait selection for ethanol consumption and withdrawal: genetic and transcriptional network effects. | Metten P et al. | โ | 2014 | โ |
| Effects of Acute Prenatal Exposure to Ethanol on microRNA Expression are Ameliorated by Social Enrichment. | Ignacio C et al. | โ | 2014 | โ |
| Genetic risk prediction and neurobiological understanding of alcoholism. | Levey DF et al. | โ | 2014 | โ |
| Modest long-term ethanol consumption affects expression of neurotransmitter receptor genes in the rat nucleus accumbens. | Jonsson S et al. | โ | 2014 | โ |
| Performing integrative functional genomics analysis in GeneWeaver.org. | Jay JJ et al. | โ | 2014 | โ |
| The alcohol-preferring (P) and high-alcohol-drinking (HAD) rats--animal models of alcoholism. | McBride WJ et al. | โ | 2014 | โ |
| Type 1 equilibrative nucleoside transporter regulates astrocyte-specific glial fibrillary acidic protein expression in the striatum. | Hinton DJ et al. | โ | 2014 | โ |
| Behavioral, Biochemical, and Molecular Indices of Stress are Enhanced in Female Versus Male Rats Experiencing Nicotine Withdrawal. | Torres OV et al. | โ | 2013 | โ |
| Brief alcohol exposure alters transcription in astrocytes via the heat shock pathway. | Pignataro L et al. | โ | 2013 | โ |
| Changes in gene expression within the ventral tegmental area following repeated excessive binge-like alcohol drinking by alcohol-preferring (P) rats. | McBride WJ et al. | โ | 2013 | โ |
| Gene expression in brain and liver produced by three different regimens of alcohol consumption in mice: comparison with immune activation. | Osterndorff-Kahanek E et al. | โ | 2013 | โ |
| Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption. | McBride WJ et al. | โ | 2013 | โ |
| Microglial activation is not equivalent to neuroinflammation in alcohol-induced neurodegeneration: The importance of microglia phenotype. | Marshall SA et al. | โ | 2013 | โ |
| Modeling the diagnostic criteria for alcohol dependence with genetic animal models. | Crabbe JC et al. | โ | 2013 | โ |
| Neurobiological signatures of alcohol dependence revealed by protein profiling. | Gorini G et al. | โ | 2013 | โ |
| Potential translational targets revealed by linking mouse grooming behavioral phenotypes to gene expression using public databases. | Roth A et al. | โ | 2013 | โ |
| Adenosine and glutamate signaling in neuron-glial interactions: implications in alcoholism and sleep disorders. | Nam HW et al. | โ | 2012 | โ |
| Compulsive drug use and its neural substrates. | Lesscher HM et al. | โ | 2012 | โ |
| Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption. | McBride WJ et al. | โ | 2012 | โ |
| Gene Expression Under the Influence: Transcriptional Profiling of Ethanol in the Brain. | Contet C | โ | 2012 | โ |
| Implications of central immune signaling caused by drugs of abuse: mechanisms, mediators and new therapeutic approaches for prediction and treatment of drug dependence. | Coller JK et al. | โ | 2012 | โ |
| Intermittent availability of ethanol does not always lead to elevated drinking in mice. | Crabbe JC et al. | โ | 2012 | โ |
| The genetics of alcohol dependence: advancing towards systems-based approaches. | Palmer RH et al. | โ | 2012 | โ |
| The neurobiology of binge-like ethanol drinking: evidence from rodent models. | Sprow GM et al. | โ | 2012 | โ |
| Translational behaviour-genetic studies of alcohol: are we there yet? | Crabbe JC | โ | 2012 | โ |
| Anti-apoptosis and cell survival: a review. | Portt L et al. | โ | 2011 | โ |
| Ceftriaxone, a beta-lactam antibiotic, reduces ethanol consumption in alcohol-preferring rats. | Sari Y et al. | โ | 2011 | โ |
| Convergent functional genomic studies of ฯ-3 fatty acids in stress reactivity, bipolar disorder and alcoholism. | Le-Niculescu H et al. | โ | 2011 | โ |
| Expression of the gene encoding the ghrelin receptor in rats selected for differential alcohol preference. | Landgren S et al. | โ | 2011 | โ |
| Modeling binge-like ethanol drinking by peri-adolescent and adult P rats. | Bell RL et al. | โ | 2011 | โ |
| Molecular targets of alcohol action: Translational research for pharmacotherapy development and screening. | Gorini G et al. | โ | 2011 | โ |
| Preclinical studies of alcohol binge drinking. | Crabbe JC et al. | โ | 2011 | โ |
| Sex-Specific Regulation of Depression, Anxiety-Like Behaviors and Alcohol Drinking in Mice Lacking ENT1. | Ruby CL et al. | โ | 2011 | โ |
| Type 1 equilibrative nucleoside transporter regulates ethanol drinking through accumbal N-methyl-D-aspartate receptor signaling. | Nam HW et al. | โ | 2011 | โ |
| Upregulated vimentin suggests new areas of neurodegeneration in a model of an alcohol use disorder. | Kelso ML et al. | โ | 2011 | โ |
| An essential role for adenosine signaling in alcohol abuse. | Ruby CL et al. | โ | 2010 | โ |
| Changes in gene expression in regions of the extended amygdala of alcohol-preferring rats after binge-like alcohol drinking. | McBride WJ et al. | โ | 2010 | โ |
| Genetic variation and brain gene expression in rodent models of alcoholism implications for medication development. | Bjรถrk K et al. | โ | 2010 | โ |