Review: DNA methylation and alcohol use disorders: Progress and challenges.

paper Cited Public

Authors: Zhang, Huiping; Gelernter, Joel
Year: 2017
Journal: The American journal on addictions
PMID: 27759945
DOI: 10.1111/ajad.12465
PMCID: PMC6003819

#	Section	Preview
20	RESULTS — AUD-Associated DNA Methylation Changes Across the Genome	Two published studies applied the Illumina Infinium HumanMethylation27 BeadChip assay to identify…
21	RESULTS — AUD-Associated DNA Methylation Changes Across the Genome	Philibert et al. performed two EWAS studies on AUDs using the Illumina Infinium HumanMethylation450…
22	RESULTS — AUD-Associated DNA Methylation Changes Across the Genome	after 4 weeks of treatment. Additionally, Zhao et al. compared peripheral blood DNA methylome…
23	RESULTS — DNA Methylation-Gene Expression Correlations in AUD Subjects	DNA methylation, as a common epigenetic mark in the genome, plays a critical role in the regulation…
24	RESULTS — DNA Methylation-Gene Expression Correlations in AUD Subjects	We examined alcohol drinking-induced brain Htr3a DNA methylation and gene expression alterations…
25	RESULTS — DNA Methylation-Gene Expression Correlations in AUD Subjects	dynorphins in postmortem dorsolateral prefrontal cortex (dl-PFC) of AUD subjects, thus, supporting…
26	RESULTS — The Interactive Effect of SNP Genotypes and CpG Methylation on AUDs	To investigate the mechanisms of how DNA methylation regulates gene transcription, we must first…
27	RESULTS — The Interactive Effect of SNP Genotypes and CpG Methylation on AUDs	blood lymphocytes of 191 subjects at high risk for AUDs. They reported that women homozygous for the…
28	RESULTS — The Interactive Effect of SNP Genotypes and CpG Methylation on AUDs	Overall, some of the above studies have demonstrated significant associations between DNA…
29	DISCUSSION	As mentioned earlier, epigenetic variation (such as DNA methylation) may be an explanation for some…
30	DISCUSSION — Application of Novel DNA Methylome Profiling Technologies in Epigenetic Studies of AUDs	A number of studies have analyzed AUD-associated DNA methylation changes using DNA methylation…
31	DISCUSSION — Application of Novel DNA Methylome Profiling Technologies in Epigenetic Studies of AUDs	which covers more than 850K CpGs across the genome. Additionally, whole genome bisulfite sequencing…
32	DISCUSSION — Investigation of AUD-Associated DNA Methylomic Alterations in Reward-Related Brain Region	To enhance our understanding of the etiology of AUDs, we need to examine AUD-associated DNA…
33	DISCUSSION — Investigation of AUD-Associated DNA Methylomic Alterations in Reward-Related Brain Region	promoter region of Htr3a in nine reward-related brain regions of mice and showed a wide range of…
34	DISCUSSION — Investigation of AUD-Associated DNA Methylomic Alterations in Reward-Related Brain Region	To date, only three small published studies have analyzed DNA methylation changes in postmortem…
35	DISCUSSION — Investigation of AUD-Associated DNA Methylomic Alterations in Reward-Related Brain Region	brain epigenetic studies have generated interesting findings, they are limited by the small sizes of…
36	DISCUSSION — Unraveling the Mechanisms of Differential DNA Methylation in AUD Subjects	As noted in our review of the literature, a number of human studies have supported altered DNA…
37	DISCUSSION — Unraveling the Mechanisms of Differential DNA Methylation in AUD Subjects	The first approach is to use animal models to validate DNA methylation/gene expression alterations…
38	DISCUSSION — Unraveling the Mechanisms of Differential DNA Methylation in AUD Subjects	The second approach is to use stem cell-derived neurons to model DNA methylation/gene expression…
39	DISCUSSION — Unraveling the Mechanisms of Differential DNA Methylation in AUD Subjects	The third approach, quite different in its goals from the methods described above, is to perform…

Citation	PMID	DOI	Status
Agrawal, A et al., Alcohol Res, 2012, Identifying genetic variation for alcohol dependence	23134043	10.35946/arcr.v34.3.02	Primary
Alegria-Torres, JA et al., Epigenomics, 2011, Epigenetics and lifestyle	22122337	10.2217/epi.11.22	Cited
Antequera, F et al., Proc Natl Acad Sci USA, 1993, Number of CpG islands and genes in human and mouse	7505451	10.1073/pnas.90.24.11995	Cited
Baik, I et al., Am J Clin Nutr, 2011, Genome-wide association studies identify genetic loci related to alcohol consumption in Korean men	21270382	10.3945/ajcn.110.001776	Cited
Ball, D, Addiction, 2008, Addiction science and its genetics	18042191	10.1111/j.1360-0443.2007.02061.x	Cited
Barker, JM et al., Alcohol Clin Exp Res, 2013, Ethanol-induced Htr3a promoter methylation changes in mouse blood and brain	22834954	10.1111/j.1530-0277.2012.01906.x	Cited
Bayerlein, K et al., Alcohol, 2011, Orexin A expression and promoter methylation in patients with alcohol dependence comparing acute and protracted withdrawal	21621370	10.1016/j.alcohol.2011.02.306	Cited
Bell, JT et al., Genome Biol, 2011, DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines	21251332	10.1186/gb-2011-12-1-r10	Cited
Biermann, T et al., J Neural Transm (Vienna), 2009, N-methyl-D-aspartate 2b receptor subtype (NR2B) promoter methylation in patients during alcohol withdrawal	19350219	10.1007/s00702-009-0212-2	Cited
Bird, A, Genes Dev, 2002, DNA methylation patterns and epigenetic memory	11782440	10.1101/gad.947102	Cited
Bleich, S et al., Alcohol Clin Exp Res, 2006, Epigenetic DNA hypermethylation of the HERP gene promoter induces down-regulation of its mRNA expression in patients with alcohol dependence	16573575	10.1111/j.1530-0277.2006.00068.x	Cited
Bocker, MT et al., Blood, 2011, Genome-wide promoter DNA methylation dynamics of human hematopoietic progenitor cells during differentiation and aging	21427290	10.1182/blood-2011-01-331926	Cited
Bonsch, D et al., J Neural Transm (Vienna), 2004, Homocysteine associated genomic DNA hypermethylation in patients with chronic alcoholism	15565495	10.1007/s00702-004-0232-x	Cited
Bonsch, D et al., J Neural Transm (Vienna), 2006, Lowered DNA methyltransferase (DNMT-3b) mRNA expression is associated with genomic DNA hypermethylation in patients with chronic alcoholism	16463117	10.1007/s00702-005-0413-2	Cited
Bonsch, D et al., Neuroreport, 2005, DNA hypermethylation of the alpha synuclein promoter in patients with alcoholism	15671870	10.1097/00001756-200502080-00020	Cited
Brennand, KJ et al., Nature, 2011, Modelling schizophrenia using human induced pluripotent stem cells	21490598	10.1038/nature09915	Cited
Bruckmann, C et al., Epigenetics, 2016, Validation of differential GDAP1 DNA methylation in alcohol dependence and its potential function as a biomarker for disease severity and therapy outcome	27128683	10.1080/15592294.2016.1179411	Cited
Cho, KS et al., Curr Opin Genet Dev, 2004, Advances in chromatin remodeling and human disease	15172675	10.1016/j.gde.2004.04.015	Cited
Crocq, MA, Dialogues Clin Neurosci, 2003, Alcohol, nicotine, caffeine, and mental disorders	22033899	10.31887/DCNS.2003.5.2/macrocq	Cited
Culverhouse, RC et al., Hum Genet, 2011, Uncovering hidden variance: Pair-wise SNP analysis accounts for additional variance in nicotine dependence	21079997	10.1007/s00439-010-0911-7	Cited
Davies, MN et al., Genome Biol, 2012, Functional annotation of the human brain methylome identifies tissue-specific epigenetic variation across brain and blood	22703893	10.1186/gb-2012-13-6-r43	Cited
de Rooij, SR et al., Psychoneuroendocrinology, 2012, Associations between DNA methylation of a glucocorticoid receptor promoter and acute stress responses in a large healthy adult population are largely explained by lifestyle and educational differences	21978868	10.1016/j.psyneuen.2011.09.010	Cited
Dedova, I et al., Int J Mol Sci, 2009, The importance of brain banks for molecular neuropathological research: The New South Wales Tissue Resource Centre experience	19333451	10.3390/ijms10010366	Cited
Delcuve, GP et al., J Cell Physiol, 2009, Epigenetic control	19127539	10.1002/jcp.21678	Cited
Ehrlich, M et al., Nucleic Acids Res, 1982, Amount and distribution of 5-methylcytosine in human DNA from different types of tissues of cells	7079182	10.1093/nar/10.8.2709	Cited
Enoch, MA et al., Curr Psychiatry Rep, 2001, The genetics of alcoholism and alcohol abuse	11276410	10.1007/s11920-001-0012-3	Cited
Fan, L et al., Addict Biol, 2004, Genetic study of alcoholism and novel gene expression in the alcoholic brain	15203434	10.1080/13556210410001674040	Cited
Gelernter, J et al., Mol Psychiatry, 2014, Genome-wide association study of alcohol dependence: Significant findings in African- and European-Americans including novel risk loci	24166409	10.1038/mp.2013.145	Primary
Gibbs, JR et al., PLoS Genet, 2010, Abundant quantitative trait loci exist for DNA methylation and gene expression in human brain	20485568	10.1371/journal.pgen.1000952	Cited
Girardot, M et al., Epigenomics, 2013, Epigenetic deregulation of genomic imprinting in humans: Causal mechanisms and clinical implications	24283884	10.2217/epi.13.66	Cited
Glahn, A et al., Eur Addict Res, 2014, Alcohol-induced changes in methylation status of individual CpG sites, and serum levels of vasopressin and atrial natriuretic peptide in alcohol-dependent patients during detoxification treatment	24356727	10.1159/000357473	Cited
Goldman, D et al., Nat Rev Genet, 2005, The genetics of addictions: Uncovering the genes	15995696	10.1038/nrg1635	Cited
Grant, BF et al., Arch Gen Psychiatry, 2004, Nicotine dependence and psychiatric disorders in the United States: Results from the national epidemiologic survey on alcohol and related conditions	15520358	10.1001/archpsyc.61.11.1107	Cited
Grant, BF et al., JAMA Psychiatry, 2015, Epidemiology of DSM-5 alcohol use disorder: Results from the national epidemiologic survey on alcohol and related conditions III	26039070	10.1001/jamapsychiatry.2015.0584	Cited
Gupta, K et al., Neurosci Lett, 2013, NMDA receptor-dependent glutamate excitotoxicity in human embryonic stem cell-derived neurons	23518152	10.1016/j.neulet.2013.03.010	Cited
Harlaar, N et al., Alcohol Clin Exp Res, 2014, Methylation of a CpG site near the ALDH1A2 gene is associated with loss of control over drinking and related phenotypes	24236815	10.1111/acer.12312	Cited
Harlaar, N et al., Drug Alcohol Depend, 2013, Alcohol and the methylome: Design and analysis considerations for research using human samples	23968814	10.1016/j.drugalcdep.2013.07.026	Cited
Hasin, DS et al., Soc Psychiatry Psychiatr Epidemiol, 2015, The national epidemiologic survey on alcohol and related conditions (NESARC) waves 1 and 2: Review and summary of findings	26210739	10.1007/s00127-015-1088-0	Cited
Hatada, I et al., Oncogene, 2006, Genome-wide profiling of promoter methylation in human	16407832	10.1038/sj.onc.1209331	Cited
Hillemacher, T et al., J Psychiatr Res, 2009, Promoter specific methylation of the dopamine transporter gene is altered in alcohol dependence and associated with craving	18504048	10.1016/j.jpsychires.2008.04.006	Cited
Hillemacher, T et al., Psychoneuroendocrinology, 2009, Epigenetic regulation and gene expression of vasopressin and atrial natriuretic peptide in alcohol withdrawal	19046820	10.1016/j.psyneuen.2008.10.019	Cited
Hsieh, TF, Methods Mol Biol, 2015, Whole-genome DNA methylation profiling with nucleotide resolution	25757766	10.1007/978-1-4939-2444-8_2	Cited
Huang, H et al., BMC Bioinformatics, 2013, Age-adjusted nonparametric detection of differential DNA methylation with case-control designs	23497201	10.1186/1471-2105-14-86	Cited
Jasiewicz, A et al., J Psychiatr Res, 2015, DAT1 methylation changes in alcohol-dependent individuals vs. controls	25862379	10.1016/j.jpsychires.2015.03.007	Cited
Joubert, BR et al., Am J Hum Genet, 2016, DNA methylation in newborns and maternal smoking in pregnancy: Genome-wide consortium meta-analysis	27040690	10.1016/j.ajhg.2016.02.019	Cited
Kass, SU et al., Trends Genet, 1997, How does DNA methylation repress transcription?	9385841	10.1016/s0168-9525(97)01268-7	Cited
Kerkel, K et al., Nat Genet, 2008, Genomic surveys by methylation-sensitive SNP analysis identify sequence-dependent allele-specific DNA methylation	18568024	10.1038/ng.174	Cited
Khalid, O et al., Stem Cell Res, 2014, Gene expression signatures affected by alcohol-induced DNA methylomic deregulation in human embryonic stem cells	24751885	10.1016/j.scr.2014.03.009	Cited
Kim, DS et al., Biomarkers, 2016, Effect of alcohol consumption on peripheral blood Alu methylation in Korean men	26846433	10.3109/1354750X.2015.1134661	Cited
Kim, KY et al., Proc Natl Acad Sci USA, 2011, Neuronal maturation defect in induced pluripotent stem cells from patients with Rett syndrome	21807996	10.1073/pnas.1018979108	Cited
Klose, RJ et al., Trends Biochem Sci, 2006, Genomic DNA methylation: The mark and its mediators	16403636	10.1016/j.tibs.2005.12.008	Cited
Kohnke, MD, Biochem Pharmacol, 2008, Approach to the genetics of alcoholism: A review based on pathophysiology	17669369	10.1016/j.bcp.2007.06.021	Cited
Kokame, K et al., J Biol Chem, 2000, Herp a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress	10922362	10.1074/jbc.M002063200	Cited
Laird, PW, Nat Rev Genet, 2010, Principles and challenges of genomewide DNA methylation analysis	20125086	10.1038/nrg2732	Cited
Lam, LL et al., Proc Natl Acad Sci USA, 2012, Factors underlying variable DNA methylation in a human community cohort	23045638	10.1073/pnas.1121249109	Cited
Lan, X et al., Front Genet, 2013, Maternal diet during pregnancy induces gene expression and DNA methylation changes in fetal tissues in sheep	23577020	10.3389/fgene.2013.00049	Cited
Li, YF et al., Chest, 2005, Maternal and grandmaternal smoking patterns are associated with early childhood asthma	15821200	10.1378/chest.127.4.1232	Cited
Liu, J et al., PLoS ONE, 2010, A study of the influence of sex on genome wide methylation	20386599	10.1371/journal.pone.0010028	Cited
Ma, L et al., Cell Stem Cell, 2012, Human embryonic stem cell-derived GABA neurons correct locomotion deficits in quinolinic acid-lesioned mice	22424902	10.1016/j.stem.2012.01.021	Cited
Manzardo, AM et al., Gene, 2012, Global DNA promoter methylation in frontal cortex of alcoholics and controls	22353363	10.1016/j.gene.2012.01.096	Cited
Maunakea, AK et al., Nature, 2010, Conserved role of intragenic DNA methylation in regulating alternative promoters	20613842	10.1038/nature09165	Cited
McLean, PJ et al., J Biol Chem, 2000, Membrane association and protein conformation of alpha-synuclein in intact neurons. Effect of Parkinson’s disease-linked mutations	10722726	10.1074/jbc.275.12.8812	Cited
Muschler, MA et al., J Neural Transm (Vienna), 2010, DNA methylation of the POMC gene promoter is associated with craving in alcohol dependence	20191296	10.1007/s00702-010-0378-7	Cited
Nadeau, JH, Genome Biol, 2015, The nature of evidence for and against epigenetic inheritance	26162370	10.1186/s13059-015-0709-y	Cited
Nan, X et al., Nature, 1998, Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex	9620804	10.1038/30764	Cited
Nielsen, DA et al., Hum Genet, 2010, Ethnic diversity of DNA methylation in the OPRM1 promoter region in lymphocytes of heroin addicts	20237803	10.1007/s00439-010-0807-6	Cited
Nieratschker, V et al., Addict Biol, 2014, Epigenetic alteration of the dopamine transporter gene in alcohol-dependent patients is associated with age	22506971	10.1111/j.1369-1600.2012.00459.x	Cited
Nieratschker, V et al., J Mol Psychiatry, 2013, Genetics and epigenetics of alcohol dependence	25408904	10.1186/2049-9256-1-11	Cited
Ono, H et al., Cancer Sci, 2012, Association of dietary and genetic factors related to one-carbon metabolism with global methylation level of leukocyte DNA	22957669	10.1111/cas.12013	Cited
Park, BL et al., Hum Genet, 2013, Extended genetic effects of ADH cluster genes on the risk of alcohol dependence: From GWAS to replication	23456092	10.1007/s00439-013-1281-8	Cited
Philibert, RA et al., Am J Med Genet B Neuropsychiatr Genet, 2008, MAOA methylation is associated with nicotine and alcohol dependence in women	18454435	10.1002/ajmg.b.30778	Cited
Philibert, RA et al., Am J Med Genet B Neuropsychiatr Genet, 2008, The relationship of 5HTT (SLC6A4) methylation and genotype on mRNA expression and liability to major depression and alcohol dependence in subjects from the Iowa adoption studies	17987668	10.1002/ajmg.b.30657	Cited
Philibert, RA et al., Epigenetics, 2014, A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs	25147915	10.4161/epi.32252	Cited
Philibert, RA et al., Front Genet, 2012, The impact of recent alcohol use on genome wide DNA methylation signatures	22514556	10.3389/fgene.2012.00054	Cited
Quillen, EE et al., Am J Med Genet Part B Neuropsychiatr Genet, 2014, ALDH2 is associated to alcohol dependence and is the major genetic determinant of “daily maximum drinks” in a GWAS study of an isolated rural chinese sample	24277619	10.1002/ajmg.b.32213	Cited
Rehan, VK et al., BMC Med, 2012, Perinatal nicotine exposure induces asthma in second generation offspring	23106849	10.1186/1741-7015-10-129	Cited
Reinius, LE et al., PLoS ONE, 2012, Differential DNA methylation in purified human blood cells: Implications for cell lineage and studies on disease susceptibility	22848472	10.1371/journal.pone.0041361	Cited
Renthal, W et al., Trends Mol Med, 2008, Epigenetic mechanisms in drug addiction	18635399	10.1016/j.molmed.2008.06.004	Cited
Robertson, KD, Nat Rev Genet, 2005, DNA methylation and human disease	16136652	10.1038/nrg1655	Cited
Sakurai, T et al., Cell, 1998, Orexins and orexin receptors: A family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior	9491897	10.1016/s0092-8674(00)80949-6	Cited
Saxonov, S et al., Proc Natl Acad Sci USA, 2006, A genome-wide analysis of CpG dinucleotides in the human genome distinguishes two distinct classes of promoters	16432200	10.1073/pnas.0510310103	Cited
Schalkwyk, LC et al., Am J Hum Genet, 2010, Allelic skewing of DNA methylation is widespread across the genome	20159110	10.1016/j.ajhg.2010.01.014	Cited
Schumann, G et al., Proc Natl Acad Sci USA, 2011, Genome-wide association and genetic functional studies identify autism susceptibility candidate 2 gene (AUTS2) in the regulation of alcohol consumption	21471458	10.1073/pnas.1017288108	Primary
Shoemaker, R et al., Genome Res, 2010, Allele-specific methylation is prevalent and is contributed by CpG-SNPs in the human genome	20418490	10.1101/gr.104695.109	Cited
Smith, AK et al., Am J Med Genet B Neuropsychiatr Genet, 2015, DNA extracted from saliva for methylation studies of psychiatric traits: Evidence tissue specificity and relatedness to brain	25355443	10.1002/ajmg.b.32278	Cited
Soubry, A et al., BMC Med, 2013, Paternal obesity is associated with IGF2 hypomethylation in newborns: Results from a Newborn Epigenetics Study (NEST) cohort	23388414	10.1186/1741-7015-11-29	Cited
Starkman, BG et al., Alcohol Res, 2012, Epigenetics-beyond the genome in alcoholism	23134045	10.35946/arcr.v34.3.04	Cited
Stevens, M et al., Genome Res, 2013, Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods	23804401	10.1101/gr.152231.112	Cited
Stirzaker, C et al., Trends Genet, 2014, Mining cancer methylomes: Prospects and challenges	24368016	10.1016/j.tig.2013.11.004	Cited
Supic, G et al., Nutr Cancer, 2013, Epigenetics: A new link between nutrition and cancer	23909721	10.1080/01635581.2013.805794	Cited
Sutherland, JE et al., Ann N Y Acad Sci, 2003, Epigenetics and the environment	12724220	10.1111/j.1749-6632.2003.tb05970.x	Cited
Taqi, MM et al., Addict Biol, 2011, Prodynorphin CpG-SNPs associated with alcohol dependence: Elevated methylation in the brain of human alcoholics	21521424	10.1111/j.1369-1600.2011.00323.x	Cited
Treutlein, J et al., Arch Gen Psychiatry, 2009, Genome-wide association study of alcohol dependence	19581569	10.1001/archgenpsychiatry.2009.83	Cited
Tycko, B, Human Mol Genet, 2010, Allele-specific DNA methylation: Beyond imprinting	20855472	10.1093/hmg/ddq376	Cited
Tyrka, AR et al., PLoS ONE, 2012, Childhood adversity and epigenetic modulation of the leukocyte glucocorticoid receptor: Preliminary findings in healthy adults	22295073	10.1371/journal.pone.0030148	Cited
Unger, T et al., Br J Clin Pharmacol, 1990, Atrial natriuretic peptide (ANP) as a neuropeptide: Interaction with angiotensin II on volume control and renal sodium handling	2148493	10.1111/j.1365-2125.1990.tb05473.x	Cited
Unternaehrer, E et al., Transl Psychiatry, 2012, Dynamic changes in DNA methylation of stress-associated genes (OXTR, BDNF) after acute psychosocial stress	22892716	10.1038/tp.2012.77	Cited
Varley, KE et al., Genome Res, 2013, Dynamic DNA methylation across diverse human cell lines and tissues	23325432	10.1101/gr.147942.112	Cited
Wang, F et al., Sci Rep, 2016, DNA co-methylation modules in postmortem prefrontal cortex tissues of European Australians with alcohol use disorders	26763658	10.1038/srep19430	Cited
Weng, JT et al., Comput Biol Med, 2015, Integrative epigenetic profiling analysis identifies DNA methylation changes associated with chronic alcohol consumption	25555412	10.1016/j.compbiomed.2014.12.003	Cited
Wiers, CE et al., Transl Psychiatry, 2015, Effects of depressive symptoms and peripheral DAT methylation on neural reactivity to alcohol cues in alcoholism	26418276	10.1038/tp.2015.141	Cited
Xiang, Y et al., PLoS ONE, 2015, Ethanol upregulates NMDA receptor subunit gene expression in human embryonic stem cell-derived cortical neurons	26266540	10.1371/journal.pone.0134907	Cited
Yaish, MW et al., Methods Mol Biol, 2014, Global DNA methylation analysis using methyl-sensitive amplification polymorphism (MSAP)	24057373	10.1007/978-1-62703-580-4_16	Cited
Yang, AS et al., Nucleic Acids Res, 2004, A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements	14973332	10.1093/nar/gnh032	Cited
Yang, BZ et al., Am J Prev Med, 2013, Child abuse and epigenetic mechanisms of disease risk	23332324	10.1016/j.amepre.2012.10.012	Cited
Yang, C et al., Hum Genet, 2013, Exploring the genetic architecture of alcohol dependence in African-Americans via analysis of a genomewide set of common variants	24297757	10.1007/s00439-013-1399-8	Cited
Zhang, D et al., Am J Hum Genet, 2010, Genetic control of individual differences in gene-specific methylation in human brain	20215007	10.1016/j.ajhg.2010.02.005	Cited
Zhang, FF et al., Epigenetics, 2011, Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood	21739720	10.4161/epi.6.5.15335	Cited
Zhang, H et al., Alcohol Clin Exp Res, 2013, Array-based profiling of DNA methylation changes associated with alcohol dependence	22924764	10.1111/j.1530-0277.2012.01928.x	Cited
Zhang, H et al., Hum Genet, 2014, Identification of methylation quantitative trait loci (mQTLs) influencing promoter DNA methylation of alcohol dependence risk genes	24889829	10.1007/s00439-014-1452-2	Cited
Zhang, H et al., J Hum Genet, 2012, Hypermethylation of OPRM1 promoter region in European Americans with alcohol dependence	22914673	10.1038/jhg.2012.98	Cited
Zhang, H et al., PLoS ONE, 2013, Profiling of childhood adversity-associated DNA methylation changes in alcoholic patients and healthy controls	23799031	10.1371/journal.pone.0065648	Cited
Zhang, R et al., Addict Biol, 2013, Genome-wide DNA methylation analysis in alcohol dependence	23387924	10.1111/adb.12037	Cited
Zhao, R et al., Asia Pac Psychiatry, 2013, Genome-wide DNA methylation patterns in discordant sib pairs with alcohol dependence	23857790	10.1111/appy.12010	Cited
Zhu, ZZ et al., Int J Epidemiol, 2012, Predictors of global methylation levels in blood DNA of healthy subjects: A combined analysis	20846947	10.1093/ije/dyq154	Cited

In this knowledge base

Title	Year	PMID
Conclusion: Special issue on genetic and alcohol use disorder research with diverse racial/ethnic groups: Key findings and potential next steps.	2017	28745446
Introduction: Special issue on genetic research of alcohol use disorder in diverse racial/ethnic populations.	2017	28672076

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Alcohol consumption is associated with widespread changes in blood DNA methylation: Analysis of cross-sectional and longitudinal data.	Dugué PA et al.	—	2021	→
Associations of Alcohol Consumption With Epigenome-Wide DNA Methylation and Epigenetic Age Acceleration: Individual-Level and Co-twin Comparison Analyses.	Stephenson M et al.	—	2021	→
Changes in DNA methylation persist over time in males with severe alcohol use disorder-A longitudinal follow-up study.	Soundararajan S et al.	—	2021	→
Genetics of substance use disorders in the era of big data.	Gelernter J et al.	—	2021	→
Impact of excessive alcohol abuse on age prediction using the VISAGE enhanced tool for epigenetic age estimation in blood.	Piniewska-Róg D et al.	—	2021	→
An analysis of the effect of mu-opioid receptor gene (OPRM1) promoter region DNA methylation on the response of naltrexone treatment of alcohol dependence.	Lin Y et al.	—	2020	→
Association of a Reproducible Epigenetic Risk Profile for Schizophrenia With Brain Methylation and Function.	Chen J et al.	—	2020	→
Associations between alcohol use and accelerated biological ageing	Bøstrand SMK et al.	—	2020	—
DNA methylation mediates the effect of cocaine use on HIV severity.	Shu C et al.	—	2020	→
Epigenetic aging is accelerated in alcohol use disorder and regulated by genetic variation in APOL2.	Luo A et al.	—	2020	→
Epigenetic changes on rat chromosome 4 contribute to disparate alcohol drinking behavior in alcohol-preferring and -nonpreferring rats.	Spence JP et al.	—	2020	→
Identification of aberrantly methylated differentially expressed genes and associated pathways in endometrial cancer using integrated bioinformatic analysis.	Liu J et al.	—	2020	→
Impact of Long-Term Alcohol Consumption and Relapse on Genome-Wide DNA Methylation Changes in Alcohol-Dependent Subjects: A Longitudinal Study.	Friedel E et al.	—	2020	→
Brain Imaging-Guided Analysis Reveals DNA Methylation Profiles Correlated with Insular Surface Area and Alcohol Use Disorder.	Zhao Y et al.	—	2019	→
DRD2 promoter methylation and measures of alcohol reward: functional activation of reward circuits and clinical severity.	Bidwell LC et al.	—	2019	→
Epigenome-Wide DNA Methylation Association Analysis Identified Novel Loci in Peripheral Cells for Alcohol Consumption Among European American Male Veterans.	Xu K et al.	—	2019	→
Lack of Association Between Serotonin Transporter Gene (SLC6A4) Promoter Methylation and Amygdala Response During Negative Emotion Processing in Individuals With Alcohol Dependence.	Muench C et al.	—	2019	→
Methamphetamine (MA) Use Induces Specific Changes in LINE-1 Partial Methylation Patterns, Which Are Associated with MA-Induced Paranoia: a Multivariate and Neuronal Network Study.	Kalayasiri R et al.	—	2019	→
Posttraumatic psychopathology and the pace of the epigenetic clock: a longitudinal investigation.	Wolf EJ et al.	—	2019	→
DNA methylation age is accelerated in alcohol dependence.	Rosen AD et al.	—	2018	→
Precision Psychiatry and Dual Disorders.	Szerman N et al.	—	2018	→
Commentary: Perspectives on alcohol-related gene and environment interplay in diverse populations.	Scott MS	—	2017	→
Conclusion: Special issue on genetic and alcohol use disorder research with diverse racial/ethnic groups: Key findings and potential next steps.	Chartier KG et al.	—	2017	→
DNA methylation signatures of chronic alcohol dependence in purified CD3<sup>+</sup> T-cells of patients undergoing alcohol treatment.	Brückmann C et al.	—	2017	→
Emerging Role of Epigenetic Mechanisms in Alcohol Addiction.	Berkel TD et al.	—	2017	→
Introduction: Special issue on genetic research of alcohol use disorder in diverse racial/ethnic populations.	Chartier KG et al.	—	2017	→