Retentive multipotency of adult dorsal root ganglia stem cells.
paper
Cited
Public
- Authors
- Singh, Rabindra P; Cheng, Ying-Hua; Nelson, Paul; Zhou, Feng C
- Year
- 2009
- Journal
- Cell transplantation
- PMID
- 19476209
- DOI
- 10.3727/096368909788237177
- PMCID
- PMC2720448
Preservation of neural stem cells (NSCs) in the adult peripheral nervous system (PNS) has recently been confirmed. However, it is not clear whether peripheral NSCs possess predestined, bona fide phenotypes or a response to innate developmental cues. In this study, we first demonstrated the longevity, multipotency, and high fidelity of sensory features of postmigrating adult dorsal root ganglia (aDRG) stem cells. Derived from aDRG and after 4-5 years in culture without dissociating, the aDRG NSCs were found capable of proliferation, expressing neuroepithelial, neuronal, and glial markers. Remarkably, these aDRG NSCs expressed sensory neuronal markers vesicular glutamate transporter 2 (VGluT2--glutamate terminals), transient receptor potential vanilloid 1 (TrpV1--capsaicin sensitive), phosphorylated 200 kDa neurofilaments (pNF200--capsaicin insensitive, myelinated), and the serotonin transporter (5-HTT), which normally is transiently expressed in developing DRG. Furthermore, in response to neurotrophins, the aDRG NSCs enhanced TrpV1 expression upon exposure to nerve growth factor (NGF), but not to brain-derived neurotrophic factor (BDNF). On the contrary, BDNF increased the expression of NeuN. Third, the characterization of aDRG NSCs was demonstrated by transplantation of red fluorescent-expressing aDRG NSCs into injured spinal cord. These cells expressed nestin, Hu, and beta-III-tubulin (immature neuronal markers), GFAP (astrocyte marker) as well as sensory neural marker TrpV1 (capsaicin sensitive) and pNF200 (mature, capsaicin insensitive, myelinated). Our results demonstrated that the postmigrating neural crest adult DRG stem cells not only preserved their multipotency but also were retentive in sensory potency despite the age and long-term ex vivo status.
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| Name | Type |
|---|---|
| 5-HT | drug |
| 5-HTT | gene |
| Acepromazine | drug |
| adeno-associated virus (AAV) local | drug |
| aDRG local | anatomy |
| aDRG NSC local | cohort |
| aDRG NSCs local | anatomy |
| aDRG NSCs local | cohort |
| aDRG NSCs local | drug |
| adult DRG NSCs local | cohort |
| adult rat DRG local | anatomy |
| adult rats | cohort |
| age | phenotype |
| Alexa 350 | drug |
| Alexa 635 | drug |
| Alexa Fluor 488 | drug |
| Antibiotic effect local | phenotype |
| astrocytes | phenotype |
| Atropine | drug |
| avidin-biotin peroxidase kit local | drug |
| Bdnf | gene |
| bFGF | drug |
| biotinylated secondary antibody local | drug |
| borate buffer local | drug |
| BrdU | drug |
| buprenorphine | drug |
| capsaicin | drug |
| capsaicin-insensitive myelinated DRG neuron local | phenotype |
| capsaicin-insensitive neuron local | phenotype |
| capsaicin-sensitive neuron local | phenotype |
| capsaicin-sensitive sensory neuron local | phenotype |
| capsaicin-sensitive sensory neurons local | phenotype |
| Cefazolin local | drug |
| cell proliferation | phenotype |
| central canal local | anatomy |
| clonal neurosphere formation local | phenotype |
| CNS | anatomy |
| control cultures local | cohort |
| corticospinal tract | anatomy |
| DAB local | drug |
| DMEM/F12 | drug |
| DMEM/F-12 media local | drug |
| DMEM/F-12 medium local | drug |
| DNASE1 local | gene |
| dopaminergic neurons | anatomy |
| dorsal columns local | anatomy |
| dorsal horn local | anatomy |
| dorsal root ganglia local | anatomy |
| Dorsal root ganglia local | anatomy |
| dorsal root ganglion | anatomy |
| DRG | anatomy |
| DRG neurons local | phenotype |
| DRG NSC local | cohort |
| DRG NSCs local | anatomy |
| DRG NSCs local | cohort |
| eDRG local | anatomy |
| eDRG local | cohort |
| eDRG NSC local | cohort |
| eDRG NSCs local | anatomy |
| eDRG NSCs local | cohort |
| EGF | drug |
| ELAVL1 | gene |
| embryonic DRG NSCs local | cohort |
| Embryonic neural crest progenitor cell local | phenotype |
| embryonic stem cells local | phenotype |
| fetal bovine serum | drug |
| FITC | drug |
| formaldehyde | drug |
| GFAP | gene |
| glial-like morphology local | phenotype |
| glutamatergic neurons | phenotype |
| gracile fasciculus local | anatomy |
| gray matter | anatomy |
| HCl | drug |
| hippocampus | anatomy |
| hydrogen peroxide | drug |
| inhibitory proteoglycans local | drug |
| injured spinal cord local | anatomy |
| isoflurane | drug |
| ketamine | drug |
| laminin | drug |
| lesion area local | anatomy |
| Lesion site local | anatomy |
| MAP-2abc local | drug |
| mechanoreceptive neuron local | phenotype |
| methanol | drug |
| methyl green local | drug |
| multipotentiality local | phenotype |
| Myelinated sensory fibers local | phenotype |
| N2 supplement | drug |
| native phenotypes local | phenotype |
| NEFH | gene |
| NES | gene |
| nestin local | drug |
| nestin | gene |
| NeuN | drug |
| NeuN-positive cells local | phenotype |
| neural crest cells local | phenotype |
| neural crest-derived stem cells local | phenotype |
| Neural crest progenitor cell local | phenotype |
| neural-like cells local | phenotype |
| neural stem cell character local | phenotype |
| neural stem cells | cohort |
| neurobasal medium | drug |
| neuroepithelial feature local | phenotype |
| neuroepithelial stem cells local | phenotype |
| neurogenesis | phenotype |
| neuronal-like morphology local | phenotype |
| Neuronal markers local | phenotype |
| neuronal maturation | phenotype |
| neuronal maturity local | phenotype |
| neurons | phenotype |
| neurospheres local | drug |
| Neurospheres local | phenotype |
| neurotrophin | drug |
| NF200 local | drug |
| NGF | drug |
| NGFR local | gene |
| nociceptive sensory neuron local | phenotype |
| normal serum local | drug |
| NSCs local | phenotype |
| NTRK1 | gene |
| NTRK2 | gene |
| NTRK3 local | gene |
| olfactory bulb | anatomy |
| opioid | drug |
| Papain | drug |
| penicillin-streptomycin | drug |
| peripheral sensory neuron local | phenotype |
| Phosphate buffer local | drug |
| picric acid local | drug |
| pNF200 local | drug |
| pNF200 local | phenotype |
| poly-D-lysine | drug |
| post-natal rats local | cohort |
| proliferation rate local | phenotype |
| proprioceptive neuron local | phenotype |
| proprio-spinal tract local | anatomy |
| protease | drug |
| protracted maturation process local | phenotype |
| quiescent neural stem cells local | phenotype |
| rats | cohort |
| RBFOX3 | gene |
| red fluorescent protein (RFP) local | drug |
| RFP local | drug |
| RFP NSCs local | cohort |
| rhodamine local | drug |
| saline | drug |
| sensory deficits | phenotype |
| sensory differentiation local | phenotype |
| Sensory differentiation local | phenotype |
| Sensory ganglia local | anatomy |
| Sensory markers local | phenotype |
| sensory neuron local | phenotype |
| Sensory neuron local | phenotype |
| sensory neurons local | phenotype |
| Sensory neurons local | phenotype |
| serotonin | drug |
| sheep serum local | drug |
| Slc17a6 | gene |
| SLC6A4 | gene |
| somatosensory cortex | anatomy |
| spinal cord | anatomy |
| spinal cord injury | anatomy |
| Sprague-Dawley local | cohort |
| Sprague-Dawley rats | cohort |
| stem cell potency local | phenotype |
| stem cell potential local | phenotype |
| stem cell preservation local | phenotype |
| stem cell proliferation local | phenotype |
| substantia nigra | anatomy |
| subventricular zone | anatomy |
| Supplemental hydration local | phenotype |
| synaptic plasticity | phenotype |
| T10 local | anatomy |
| T10 transection site local | anatomy |
| Thoracic vertebra T10 local | anatomy |
| Thoracic vertebra T11 local | anatomy |
| Thoracic vertebra T9 local | anatomy |
| trigeminal ganglion | anatomy |
| Tris-HCl buffer | drug |
| Triton X-100 | drug |
| TrpV1 | gene |
| TrpV1 immunostaining local | phenotype |
| TRPV1-positive cells local | phenotype |
| trypsin | drug |
| TUBB3 | gene |
| type 1 astrocyte-like cell local | phenotype |
| type 2 astrocyte-like cell local | phenotype |
| UV filter local | drug |
| ventral horn local | anatomy |
| ventrolateral white matter local | anatomy |
| ventromedial funiculus local | anatomy |
| vGLUT2 | gene |
| white matter | anatomy |
| Ξ²-III-Tubulin local | drug |
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