Item-level analyses reveal genetic heterogeneity in neuroticism.

Genetic correlations between the 12 neuroticism items. Genetic correlations between the 12 neuroticism items within both samples (sample 1 is presented in the upper triangle and sample 2 in the bottom triangle). The genetic correlations of the same items measured in both samples are presented on the diagonal. All reported genetic correlations, computed with bivariate LD score regression, are significantly different from zero after Bonferroni correction (P < 2.22 × 10−4). For the genetic correlations between the same items (diagonal) all 95% confidence intervals included 1, emphasizing concordance in genetic signal across samples. For all other, cross-phenotype genetic correlations, none of the 95% confidence intervals included 1, indicating that all genetic correlations deviated from unity (Supplementary Data 2). See Supplementary Table 1 for a description of the item labels

Circle plot of statistically associated gene-sets of the 7,297 gene-sets tested (MsigDB gene-sets and gene-expression profiles). This circle plot presents all gene-sets that were significantly associated (Bonferroni corrected P-value threshold: 0.05/7,297 = 6.85 × 10−6) to at least one of the individual neuroticism items or to the sum-score. Each tract connects a gene-set to the phenotype with which it is associated. Tracts colored in red indicate gene-sets that were associated to individual items, but not to the sum-score. In contrast, tracts colored in yellow indicate gene-sets that were associated to the neuroticism sum-score, but not to any of the individual items. Blue tracts refer to gene-sets that were found to be associated to the sum-score and at least one of the individual items. See Supplementary Data 47 for a description of the gene-sets included in this figure. See Supplementary Table 1 for a description of the item labels

Genetic correlations of 12 neuroticism items, the sum-score and the two item clusters with 33 external traits. Genetic correlations of neuroticism items (see Supplementary Table 1 for a description of the item labels), sum-score, and the item clusters depressed affect (CLUSTER: DEPRAFF) and worry (CLUSTER: WORRY; x axis) with 33 other traits (y axis; Supplementary Data 49) computed with cross-trait LD score regression. P values and 95% confidence intervals for the rg’s are provided in Supplementary Data 50. Exact values of the genetic correlations are shown in the cells. *P < 0.01; **Bonferroni corrected P-value threshold: P < 1.01 × 10−4 (0.05/(15 × 33))