Changes in gene expression in regions of the extended amygdala of alcohol-preferring rats after binge-like alcohol drinking.
paper
Cited
Public
- Authors
- McBride, William J; Kimpel, Mark W; Schultz, Jonathan A; McClintick, Jeanette N; Edenberg, Howard J; Bell, Richard L
- Year
- 2010
- Journal
- Alcohol (Fayetteville, N.Y.)
- PMID
- 20116196
- DOI
- 10.1016/j.alcohol.2009.12.001
- PMCID
- PMC2831121
The objective of this study was to determine time-course changes in gene expression within two regions of the extended amygdala after binge-like alcohol drinking by alcohol-preferring (P) rats. Adult male P rats were given 1-h access to 15 and 30% ethanol three times daily for 8 weeks. Rats (n = 10/time point for ethanol and n = 6/time point for water) were killed by decapitation 1, 6, and 24 h after the last drinking episode. RNA was prepared from individual micropunch samples of the nucleus accumbens shell (ACB-shell) and central nucleus of the amygdala (CeA); analyses were conducted with Affymetrix Rat Genome 230.2 GeneChips. Ethanol intakes were 1.5-2 g/kg for each of the three sessions. There were no genes that were statistically different between the ethanol and water control groups at any individual time point. Therefore, an overall effect, comparing the water control and ethanol groups, was determined. In the ACB-shell and CeA, there were 276 and 402 probe sets for named genes, respectively, that differed between the two groups. There were 1.5-3.6-fold more genes with increased expression than with decreased expression in the ethanol-drinking group, with most differences between 1.1- and 1.2-fold. Among the differences between the ethanol and water control groups were several significant biological processes categories that were in common between the two regions (e.g., synaptic transmission, neurite development); however, within these categories, there were few genes in common between the two regions. Overall, the results indicate that binge-like alcohol drinking by P rats produces region-dependent changes in the expression of genes that could alter transcription, synaptic function, and neuronal plasticity in the ACB-shell and CeA; within each region, different mechanisms may underlie these alterations because there were few common ethanol-responsive genes between the ACB-shell and CeA.
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| Name | Type |
|---|---|
| 23 named genes local | gene |
| 24-hr free-choice drinking study local | cohort |
| Acb | cohort |
| ACB-shell local | cohort |
| Accumbens (ACB) local | anatomy |
| Affymetrix GCOS software local | drug |
| Affymetrix kits local | drug |
| Affymetrix Model 3000 scanner local | drug |
| Affymetrix Rat Genome 230 2.0 GeneChips local | drug |
| Agilent 2100 Bioanalyzer | drug |
| Akt | gene |
| Alc17 local | phenotype |
| Alc4 local | phenotype |
| alcohol | phenotype |
| alcoholism | phenotype |
| alcohol preference | phenotype |
| alcohol-preferring rats | cohort |
| alcohol self-administration | phenotype |
| Alcohol Use | phenotype |
| AMPA1 local | gene |
| AMPA receptors local | gene |
| amygdala | anatomy |
| animal models | cohort |
| Axin2 local | gene |
| axonogenesis | phenotype |
| Bat5 local | gene |
| Bell et al., 2009 study local | cohort |
| binge alcohol drinking local | phenotype |
| binge-alcohol drinking local | phenotype |
| binge-like alcohol drinking local | phenotype |
| binge-like alcohol-drinking model local | phenotype |
| binge-like alcohol intake local | phenotype |
| blood alcohol concentration | phenotype |
| blood alcohol levels | phenotype |
| body weight | phenotype |
| brain | anatomy |
| C57BL/6By mice local | cohort |
| C57BL/6By mouse local | cohort |
| C57BL/6J | cohort |
| Calcium-binding proteins local | gene |
| Calm3 local | gene |
| cancer | phenotype |
| Capzb local | gene |
| Cd99 | gene |
| cDNA synthesis local | drug |
| CeA | anatomy |
| cell projection morphogenesis local | phenotype |
| cell projection morphogenesis-neurite development local | phenotype |
| central amygdala | anatomy |
| Chronic alcohol drinking local | phenotype |
| chronic alcoholism | phenotype |
| Chronic ethanol drinking local | phenotype |
| chronic free-choice alcohol drinking local | phenotype |
| Cnd1 local | gene |
| CNS region samples local | cohort |
| Collaborative Study on the Genetics of Alcoholism (COGA) | cohort |
| combined BP category local | phenotype |
| conditioning | phenotype |
| congenic rat strains local | cohort |
| Continuous 24-hr access local | phenotype |
| Continuous Access Group local | cohort |
| control group | cohort |
| controls | cohort |
| Cox6a1 | gene |
| cRNA | drug |
| Cst3 | gene |
| CYB5R3 local | gene |
| Cytokine/proinflammatory factors local | gene |
| cytoskeletal organization and biogenesis local | phenotype |
| DBA/2J | cohort |
| DCTN1 local | gene |
| DCTN2 local | gene |
| demyelination | phenotype |
| dependence-induced alcohol drinking local | phenotype |
| dependence-induced drinking | phenotype |
| Dgkg | anatomy |
| Dopamine receptor subtypes local | gene |
| dorsal hippocampus | anatomy |
| drug dependence | phenotype |
| enzyme-linked receptor protein signaling pathway local | phenotype |
| ethanol consumption | phenotype |
| Ethanol Drinking Episodes local | phenotype |
| Ethanol drinking group local | cohort |
| ethanol group | cohort |
| Ethanol groups local | cohort |
| ethanol preference | phenotype |
| ethanol preference scores local | phenotype |
| excessive alcohol consumption | phenotype |
| Excessive alcohol drinking behavior local | phenotype |
| experimental group | cohort |
| extended amygdala | anatomy |
| F2r local | gene |
| Fabp3 local | gene |
| Fau local | gene |
| Fbxo2 local | gene |
| FHIT | cohort |
| Flot1 local | gene |
| FLOT1 local | gene |
| Ftl1 local | gene |
| GABAA receptor | drug |
| GABA receptor subtypes local | gene |
| Gabbr1 | gene |
| Gabra1 | gene |
| Gabrg1 | gene |
| Gabrg2 | gene |
| GAD2 | gene |
| GeneChip® local | drug |
| gene expression | phenotype |
| Gene Expression Omnibus local | drug |
| glutamate | drug |
| Glutamate receptor subunits local | gene |
| Glutathione S-transferase subtypes local | gene |
| glycolysis | drug |
| GNAI1 local | gene |
| GNAI2 local | gene |
| GNAO | gene |
| GRIA1 | gene |
| GRIA2 | gene |
| GRIK2 | gene |
| GRIK5 | gene |
| GRIN3A | gene |
| GSE15407 local | cohort |
| HDAC4 | gene |
| high alcohol drinkers local | phenotype |
| High alcohol drinking behavior local | phenotype |
| human | cohort |
| human alcoholics | phenotype |
| hybridization cocktail local | drug |
| in vitro transcription local | drug |
| kainate receptors local | gene |
| KEGG analyses local | drug |
| learning | phenotype |
| Lewis rat local | cohort |
| limbic regions | anatomy |
| long-term potentiation | phenotype |
| long-term-potentiation local | phenotype |
| low alcohol drinkers local | phenotype |
| MAEA local | gene |
| MIF local | gene |
| motor activity | phenotype |
| Mt3 local | gene |
| MT3 local | gene |
| Mtap2 local | gene |
| MTAP2 local | gene |
| Mtmr7 | gene |
| Multiple 1-hr daily scheduled access local | phenotype |
| Multiple Scheduled Access Group local | cohort |
| Myo5a local | gene |
| Ncdn local | gene |
| neurite development local | phenotype |
| neurite development genes local | gene |
| neurogenesis | phenotype |
| neurological process local | phenotype |
| neuronal differentiation | phenotype |
| Neurotoxic consequences of chronic alcohol consumption local | phenotype |
| neurotoxic effects | phenotype |
| Neurotoxic effects of binge drinking local | phenotype |
| Nicotinic receptor subunits local | gene |
| NMDA1 local | gene |
| Nmnat1 local | gene |
| NP rats | cohort |
| Nrgn | gene |
| nucleus accumbens | anatomy |
| Nucleus Accumbens (ACB) local | anatomy |
| nucleus accumbens core | anatomy |
| nucleus accumbens shell | anatomy |
| Nudc local | gene |
| Operant ethanol self-administration study local | cohort |
| Panx3 local | gene |
| PHB local | gene |
| PHB2 local | gene |
| positive regulation of nucleic acid metabolism-DNA-dependent transcription local | phenotype |
| positive reinforcement | phenotype |
| potassium ion transport local | phenotype |
| P rats | cohort |
| Prdm2 local | gene |
| prefrontal cortex | anatomy |
| present study | cohort |
| Psmb6 local | gene |
| Ptgs1 local | gene |
| purine metabolism local | phenotype |
| Rab14 local | gene |
| Rab2b local | gene |
| rat cohort (single) local | cohort |
| rats | cohort |
| regulation of protein kinase activity-MAPKKK cascade local | phenotype |
| regulation of transmission of nerve impulse local | phenotype |
| response to external stimulus local | phenotype |
| RNA | drug |
| RNAse Zap local | drug |
| RNeasy columns local | drug |
| saccharin | drug |
| sample cleanup local | drug |
| Sanpap local | gene |
| Scheduled access drinking study local | cohort |
| second-messenger-mediated signaling local | phenotype |
| selectively bred P rats local | cohort |
| Sepw1 local | gene |
| Single cohort of rats local | cohort |
| Snap25 | gene |
| Snap29 | gene |
| Snta1 local | gene |
| Snx10 local | gene |
| striatum | anatomy |
| Stx3 local | gene |
| Sty10 local | gene |
| superior frontal cortex | anatomy |
| synaptic plasticity | phenotype |
| Syt10 | gene |
| TCF7L2 | gene |
| Thap7 local | gene |
| Thra local | gene |
| TIAM1 local | gene |
| Transcription and synaptic plasticity genes local | gene |
| translation | phenotype |
| Trizol reagent | drug |
| Txnl1 local | gene |
| Usp18 local | gene |
| Vapb local | gene |
| ventral tegmental area | anatomy |
| water | drug |
| Water control group local | cohort |
| Water Control Group local | cohort |
| Water control rats local | cohort |
| water group local | cohort |
| Water group local | cohort |
| water intake | phenotype |
| whole ACB local | anatomy |
| whole brain | anatomy |
| withdrawal effects | phenotype |
| Wnt/β-catenin signaling pathway local | drug |
| β-catenin | drug |
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